Detection of a point mutation in the sodium channel gene from pyrethroid resistant strains of cattle tick (Boophilus microplus) by mismtch-primer mediated polymerase chain reaction-restriction fragment length polymorphism (mppcrrflp)

Detection of a point mutation in the sodium channel gene from pyrethroid resistant strains of cattle tick (Boophilus microplus) by mismtch-primer mediated polymerase chain reaction-restriction fragment length polymorphism (mppcrrflp)

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This method uses a mismatching primer to create a restriction site in the mutant allele by PCR amplification. After digestion, PCR products containing wild-type and mutant alleles produce a frament length polymorphism on electrophoresis that is easily discernible

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Bibliographic Details
Main Authors: 42869 Andrew C, H.H., 10848 Industria Farmaceútica Veterinaria, México, D.F. (México), 5680 Comisión Nacional de Sanidad Agropecuaria, México, D.F. (México), Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP) México D.F., México 10906, 2693 AMPAVE, México, D.F. (México), 8248 FILASA, México, D.F. (México), 61 IICA, México, D.F. (México), 4. Seminario Internacional de Parasitología Animal Puerto Vallarta, Jalisco (México) 20-22 Oct 1999
Format: Texto biblioteca
Language:spa
Published: Puerto Vallarta, Jalisco (México) 1999
Subjects:BOOPHILUS MICROPLUS, MUTACION, PIRETRINAS SINTETICAS, AGRICULTURA,
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