Detection of a point mutation in the sodium channel gene from pyrethroid resistant strains of cattle tick (Boophilus microplus) by mismtch-primer mediated polymerase chain reaction-restriction fragment length polymorphism (mppcrrflp)
This method uses a mismatching primer to create a restriction site in the mutant allele by PCR amplification. After digestion, PCR products containing wild-type and mutant alleles produce a frament length polymorphism on electrophoresis that is easily discernible
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Main Authors: | , , , , , , , |
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Format: | Texto biblioteca |
Language: | spa |
Published: |
Puerto Vallarta, Jalisco (México)
1999
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Subjects: | BOOPHILUS MICROPLUS, MUTACION, PIRETRINAS SINTETICAS, AGRICULTURA, |
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