The use of monoclonal antibodies and cDNA for detection of plant viruses
Rapid reliable methods of virus detection are essential for the proper management of virus diseases. Two developments in the past decade, monoclonal antibodies and complementary DNA (cDNA), a now make it possible to design or selected probes that are specific for all known isolates of a virus, that can readily distinguish virus strains, or that can detect all known members of a specific virus group. These probes thus have great potential for application in disease surveys, screening germplasm for exotic viruses, screening breeding lines for virus resistance, and in post-entry detection for quarantine purpose. The principles underlying the development of monoclonal antibodies directed against structural or non-structural virus proteins, and cDNA probes, which are complementary to specific regions of viral genomes, are discussed in this paper and examples of their application in the detection of plant viruses are presented
| Main Authors: | , , , , , , |
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| Format: | Texto biblioteca |
| Language: | eng |
| Published: |
Ibadan (Nigeria) IITA
1992
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