Virus-engineered resistance: concepts, efficacy, and stability
This paper reviews four strategies being used in research on virus resistance through genetic engineering in terms of their concepts, efficacy, and stability. The most recent strategy is the use of ribozymes, which involves using catalytic RNAs to cause the cleavage of viral RNA molecules. Reports of in vitro experiments have documented cleavage of viral RNAs but there are no reports of in vivo experiments. The second strategy involves an attempt to block the translation of viral RNAs by expressing genes that encode sequences complementary to viral genes. Only a few cases have been reported, and little success achieved. The third strategic is to block virus replication, including the use of competitor sequences, subgenomic sequences, and the expression of satallite RNA. This strategy has been applied with some success in the field. The fourth and most promising strategy involves integrating a gene encoding viral coat protein (CP) into the plant genome. An increasing number of examples of the use of this strategy have been reported, and resistance specificity and efficacy have been evaluated for viruses belonging to 10 groups; furthermore, several succcessful field experiments have been conducted. The paper summarizes CP-mediated resistance and puts forward hypotheses on the possible mechanisms of resistance
| Main Authors: | , , , , , , , |
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| Format: | Texto biblioteca |
| Language: | eng |
| Published: |
Ibadan (Nigeria) IITA
1992
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