Isozyme analysis and its application in plant breeding
Plant breeders face the difficult task of having to select for traits that are often under complex genetic control and subject to edapho-climatic influences. Electrophoresis of protein extracts from plant tissue, using different kinds of support media and buffers systems, allows separation of the multiple forms of enzymes (isozymes) on the basis of charge and/or molecular size. The resulting polymorphisms are useful as genetic markers. Isozyme analysis is a powerful technique for estimating genetic variability, identifying cultivars and germplasm accessions, confirming hybrids, tracking alien chromosome segments, tagging genes, and screening backcross populations more efficiently
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| Main Authors: | , , , , , , |
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| Format: | Texto biblioteca |
| Language: | eng |
| Published: |
Ibadan (Nigeria) IITA
1992
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