RFLP mapping in cowpea
Linkage maps based on restriction fragment length polymorphisms (RFLPs) are beginning to have a major impact on crop improvement. Recognizing the many important applications of RFLPs in plant breeding, the authors of this paper are constructing an RFLP linkage map for cowpea, a major legume crop in Africa and throughout the developing world. The results indicate that DNA clones from several legume genera can be used to detect RFLPs in cowpea. While the level of DNA sequence polymorphism in this crop is relatively low, it is adequate for RFLP mapping. In a prelimary analysis of eight RFLP markers in an F2 population of 58 individuals, three markers showed skewed segregation ratios, two markers were found to be linked to one another, and another appeared to be tightly linked to a major gene controlling pod lenght
Main Authors: | , , , , , , , , , |
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Format: | Texto biblioteca |
Language: | eng |
Published: |
Ibadan (Nigeria) IITA
1992
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