Assessing relationships between Musa species using chloroplast DNA RFLPs
Taxonomic and phylogenetic determinations within the genus Musa have been established using a numerical, morphology-based scoring system. Whithin this system, however, the classification and relationships of some types are disputed. The application of chloroplast DNA (cpDNA) restriction fragment length polymorphism (RFLP) analysis to Musa taxonomy could provide valuable supplementary information abouth the classification of, and relationships between. Musa species and subspecies. Whole-cell DNA was extracted from lyophilized leaf-blade tissue and digested with various restriction enzymes, Southern blotted onto nylon membranes, and probed using radioactively labelled heterologous orchi cpDNA fragments. Phylogenies were inferred from cpDNA RFLP patterns, using Phylogenetic Analysis Using Parsimony (PAUP) software. The relationships between most species examined were as expected; however, some species, particularly M. beccarii and M. basjoo, did not conform to conventional morphology-based phylogeny
| Main Authors: | , , , , , , , |
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| Format: | Texto biblioteca |
| Language: | eng |
| Published: |
Ibadan (Nigeria) IITA
1992
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