Crioconservación de suspensiones celulares embriogénicas de Musa sp. iniciadas a partir de flores inmaduras

Suspension cultures constitutes a valuable technique for the improvement of Musa spp as a source of material for genetic transformation, somatic hybridization by protoplast fusion and massive propagation. Cryopreservation offers a promising alternative for the conservation and management of these suspensions. In the present work, an existent protocol of cryopreservation was optimized for its efficiency in embryogenic cell suspensions of Musa spp., initiated form inmature flowers. Four experiments were realized independently using celular suspensions of cv. Dominico (Musa AAB). In each experiment, one of the process of the protocol was modified: crystallization induction, pretreatment conditions, cellular recuperation, and pregrowth treatment with sucrose and lactose. The optimized protocol was applied in embryogenic cell suspensions of Musa and four out of five cultivars withstood cryopreservation (Dominico, SF 265; Currare 3, and Col 49 2.8).

Bibliographic Details

Main Authors:	132355 Yah, E., 101764 Ortíz, J.L., 129940 Vásquez Morera, N., 73587 Grapin, A., 59521 Cote, F., 4010 CATIE, Turrialba (Costa Rica). Programa de Investigación, 4. Semana Científica Turrialba (Costa Rica) 6-9 Abr 1999
Format:	biblioteca
Language:	spa
Published:	Turrialba, Costa Rica 1999
Subjects:	MUSA, CONSERVACION BIOLOGICA, CONGELACION, SUSPENSIONES CELULARES, DESARROLLO EMBRIONARIO, FITOMEJORAMIENTO, HIBRIDACION SOMATICA,
Online Access:	https://repositorio.catie.ac.cr/handle/11554/2899
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