Effects of non - thermal plasma technology on Diaporthe longicolla cultures and mechanisms involved

BACKGROUND: The Diaporthe/Phomopsis complex (D/P) is a group of soybean seed-borne fungi. The use of chemical fungicides, either for seed treatment or during the crop cycle, is the most adopted practice for treating fungal diseases caused by this complex. Worldwide, there is a search for alternative seed treatments that are less harmful to the environment than chemicals. Non-thermal plasma (NTP) is a novel seed treatment technology for pathogen removal. This research aimed to evaluate the effects of NTP on the in vitro performance of pure cultures of Diaporthe longicolla and elucidate the mechanisms underlying these effects. RESULTS: Active D. longicolla mycelium, growing in vitro, was exposed to different NTP treatments, employing a dielectric barrier discharge arrangement with different carrier gases (N2 or O2). Fungal growth, fresh biomass and colony appearance were negatively affected by plasma treatments (TN3 and TO3). Lipid peroxidation and antioxidant activities were higher in plasmatreated colonies comparison with non-exposed colonies (control). Fungal asexual spores (conidia) were also exposed to NTP, showing high susceptibility. CONCLUSION: Exposure of D. longicolla colonies to NTP severely compromised fungal biology. Ozone production during treatment and lipid peroxidation of fungal cell membranes appeared to be involved in the observed effects.

Bibliographic Details

Main Authors:	Pérez Pizá, María Cecilia, Grijalba, Pablo Enrique, Cejas, Ezequiel, Chamorro Garcés, Juan C., Ferreyra, Matías, Zilli, Carla G., Vallecorsa, Pablo, Santa Cruz, Diego Mario, Yannarelli, Gustavo G., Prevosto, Leandro, Balestrasse, Karina Beatriz
Format:	Texto biblioteca
Language:	eng
Subjects:	DIAPORTHE LONGICOLLA, SOYBEAN SEED DECAY, NON THERMAL PLASMAS, FUNGAL GROWTH, ,
Online Access:	http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=54595 http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
Tags:	Add Tag No Tags, Be the first to tag this record!