A Japanese plum Alpha - L- arabinofuranosidase / Beta - D- xylosidase gene is developmentally regulated by alternative splicing
A full-length cDNA clone named PsARF/XYL was obtained from Prunus salicina Lindl., and determined to encode a putative Alpha-L-arabinofuranosidase/Beta-D-xylosidase belonging to glycoside hydrolase [GH, EC 3.2.1.-] family 3. Two related PsARF/XYL cDNAs were amplified, one from a fully-spliced transcript [PsARF/XYLa] and another one from an intron-retained transcript [PsARF/XYLb]. The protein deduced from PsARF/XYLb is a truncated peptide at C-terminus that conserves the active-site amino acid sequence. High levels of PsARF/XYLa and PsARF/XYLb transcripts are detectable in several plant tissues. PsARF/XYLb transcripts accumulate progressively during the phase of exponential fruit growth but they become barely noticeable during on-tree ripening, or after a 6-h exposure of preclimacteric full-size plums to ethylene. In contrast, PsARF/XYLa is expressed throughout fruit development, and transcript accumulation parallels the climacteric rise in ethylene production during ripening. PsARF/XYLa expression is strongly induced in preclimacteric full-size plums after a 6-h treatment with physiologically active concentrations of ethylene. These findings suggest that PsARF/XYL gene is post-transcriptionally regulated by alternative splicing during development and that ethylene may be involved in this regulation. The isolation of a partial cDNA clone, PsARF1, is also reported. It encodes a putative cell-wall Alpha-L-arabinofuranosidase, and its transcription is rapidly inhibited by ethylene in mature green plums.
| Main Authors: | , , , |
|---|---|
| Format: | Texto biblioteca |
| Language: | spa |
| Subjects: | SPLICING EVENT, RIPENING, PRUNUS SALICINA, INTRON RETENTION, ETHYLENE, , |
| Online Access: | http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=47182 http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|