Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus
The action of protease and hemicellulase for the extraction of fractions enriched in soluble fiber from bracts and stems of Cynara cardunculus was evaluated. Using a two-factor simplex design comprising protease amounts of 0-200 ?L and hemicellulase amounts of 0-200 mg for 5 g of material, we explored the effect of a 5 h enzymatic treatment at 40 °C on the chemical composition and yield of the fractions isolated. The fractions contained inulin and pectin. In general, the protein, inulin, and polyphenol contents and also the yields were higher for fractions obtained from stems. The most marked effects were observed when enzymes were used at higher concentrations, especially for hemicellulase. The inclusion of a pre-heating step increased the yield and the inulin content for fractions isolated from bracts and stems and decreased the protein and polyphenol contents, and the galacturonic acid for bracts. These fractions, in general, contained the polyphenolic compounds monocaffeoylquinic acid, apigenin, and pinoresinol.
| Main Authors: | , , , , |
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| Format: | Texto biblioteca |
| Language: | spa |
| Subjects: | URONIC ACID, SPECTROPHOTOMETRY, PROTEINASE, PROTEIN ANALYSIS, PROTEIN, PROTEASE, POLYPHENOLS, POLYPHENOL, PECTIN, NONHUMAN, INULIN, HYDROLYSIS, HIGH PERFORMANCE LIQUID CHROMATOGRAPHY, HEMICELLULOSE, HEMICELLULASE, GALACTURONIC ACID, FLOW RATE, ENZYME ISOLATION, ENZYME ACTIVITY, ELECTROSPRAY MASS SPECTROMETRY, DIETARY FIBER, CYNARA CARDUNCULUS, CONTROLLED STUDY, CHEMICAL COMPOSITION, CARDOON, BIOMASS TO CHEMICALS, , |
| Online Access: | http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=47178 http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= |
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