Candida albicans Tpk1p and Tpk2p isoforms differentially regulate pseudohyphal development, biofilm structure, cell aggregation and adhesins expression
Candida albicans undergoes a reversible morphological transition from single yeast cells to pseudohyphal and hyphal filaments. In this organism, cAMP-dependent protein kinase [PKA], coded by two catalytic subunits [TPK1 and TPK2] and one regulatory subunit [BCY1], mediates basic cellular processes, such as the yeast-to-hypha transition and cell cycle regulation. It is known that both Tpk isoforms play positive roles in vegetative growth and filamentation, although distinct roles have been found in virulence, stress response and glycogen storage. However, little is known regarding the participation of Tpk1p and/or Tpk2p in pseudohyphal development. This point was addressed using several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in different BCY1 genetic backgrounds. We observed that under hypha-only inducing conditions, all BCY1 heterozygous strains shifted growth toward pseudohyphal morphology; however, the pseudohypha:hypha ratio was higher in strains devoid of TPK2. Under pseudohypha-only inducing conditions, strains lacking TPK2 were prone to develop short and branched pseudohyphae. In tpk2?/tpk2? strains, biofilm architecture was markedly less dense, composed of short pseudohyphae and blastospores with reduced adhesion ability to abiotic material, suggesting a significant defect in cell adherence. Immunolabelling assays showed a decreased expression of adhesins Als1p and Als3p only in the tpk2?/tpk2? strain. Complementation of this mutant with a wild-type copy of TPK2 restored all the altered functions: pseudohyphae elongation, biofilm composition, cell aggregation and adhesins expression. Our study suggests that the Tpk2p isoform may be part of a mechanism underlying not only polarized pseudohyphal morphogenesis but also cell adherence.
| Main Authors: | , , , |
|---|---|
| Format: | Texto biblioteca |
| Language: | eng |
| Subjects: | ADHESIN, ADHESINS, ALS1P PROTEIN, ALS3P PROTEIN, ANTIBODY LABELING, BCY1 PROTEIN, BIOFILM, BIOFILM ARCHITECTURE, BIOFILMS, CANDIDA ALBICANS, CELL ADHESION, CONTROLLED STUDY, CYCLIC AMP DEPENDENT PROTEIN KINASE 1, CYCLIC AMP DEPENDENT PROTEIN KINASE 2, CYCLIC AMP-DEPENDENT PROTEIN KINASES, FUNGAL GENE, FUNGAL PROTEIN, FUNGAL PROTEINS, FUNGAL STRAIN, FUNGUS GROWTH, FUNGUS HYPHAE, FUNGUS MUTANT, FUNGUS SPORE, GENE DELETION, GENE EXPRESSION REGULATION, DEVELOPMENTAL, GENE EXPRESSION REGULATION, FUNGAL, HETEROZYGOSITY, HYPHAE, MORPHOGENESIS, NONHUMAN, PKA, PROTEIN EXPRESSION, PROTEIN ISOFORMS, PSEUDOHYPHAL DEVELOPMENT, STRAIN DIFFERENCE, UNCLASSIFIED DRUG, WILD TYPE, |
| Online Access: | http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=47148 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|