Reduced faecal shedding of Escherichia coli O157 H7 in cattle following systemic vaccination with Y - intimin C 280 and EspB proteins
Enterohaemorrhagic Escherichia coli [EHEC] O157:H7 is the most prevalent EHEC serotype that has been recovered from patients with haemolytic uremic syndrome [HUS] worldwide. Vaccination of cattle, the main reservoir of EHEC O157:H7, could be a logical strategy to fight infection in humans. This study evaluated a vaccine based on the carboxyl-terminal fragment of 280 amino acids of gamma-intimin [gamma-intimin C 280] and EspB, two key colonization factors of E. coli O157:H7. Intramuscular immunization elicited significantly high levels of serum IgG antibodies against both proteins. Antigen-specific IgA and IgG were also induced in saliva, but only the IgA response was significant. Following experimental challenge with E. coli O157:H7, a significant reduction in bacterial shedding was observed in vaccinated calves, compared to control group. These promising results suggest that systemic immunization of cattle with intimin and EspB could be a feasible strategy to reduce EHEC O157:H7 faecal shedding in cattle.
| Main Authors: | , , , , , , , , , , |
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| Format: | Texto biblioteca |
| Language: | spa |
| Subjects: | E. COLI O157:H7, ESPB, INTIMIN, VACCINE, ESCHERICHIA COLI PROTEIN, IMMUNOGLOBULIN A ANTIBODY, IMMUNOGLOBULIN G ANTIBODY, PROTEIN ESPB, RECOMBINANT PROTEIN, UNCLASSIFIED DRUG, ANIMAL EXPERIMENT, ANIMAL MODEL, BACTERIAL SHEDDING, CATTLE, CONTROLLED STUDY, ESCHERICHIA COLI INFECTION, ESCHERICHIA COLI O157, IMMUNOGLOBULIN BLOOD LEVEL, MALE, NONHUMAN, ADHESINS, BACTERIAL, ANIMALS, ANTIBODIES, BACTERIAL, BACTERIAL OUTER MEMBRANE PROTEINS, CATTLE DISEASES, ESCHERICHIA COLI INFECTIONS, ESCHERICHIA COLI PROTEINS, ESCHERICHIA COLI VACCINES, FECES, INJECTIONS, INTRAMUSCULAR, RECOMBINANT PROTEINS, TREATMENT OUTCOME, VACCINATION, |
| Online Access: | http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=46851 |
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