Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons
There is limited information about the involvement of lipids and esterified fatty acids in signaling pathways during plant development. The purpose of this study was to evaluate the lipid composition and molecular species of potato [Solanum tuberosum L., cv. Spunta] stolons and to identify phosphorylated lipids in the first two developmental stages of tuber formation. Lipid profiling was determined using ESI-MS/MS, a useful method for the determination of the biosynthesis and catabolism of lipids based on their fatty acid composition. The most prevalent compound identified in this study was phosphatidic acid [PA]; digalactosyldiacylglycerol [DGDG] was the second most abundant compound. A 34:2 species was identified in PA, phosphatidylcholine [PC], phosphatidylinositol [PI], and phosphatidylethanolamine [PE]. The identification of lipid phosphorylation by kinases was revealed by the presence of the phosphorylated lipids. PA was metabolized to diacylglycerol pyrophosphate [DGPP] by phosphatidic acid kinase [PAK]. This work establishes a correlation between lipid fatty acid composition and lipid metabolism enzymes at the beginning of tuber formation and is the first report of PAK activity in the early events of potato tuber formation.
| Main Authors: | , , , , |
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| Format: | Texto biblioteca |
| Language: | eng |
| Subjects: | ELECTROSPRAY IONIZATION TANDEM MASS SPECTROMETRY, GALACTOLIPID, KINASE, PHOSPHOLIPID, POTATO, SOLANUM TUBEROSUM, BIOCHEMISTRY, ENZYMES, FATTY ACIDS, GLYCEROL, IONIZATION, MASS SPECTROMETRY, METABOLISM, PHOSPHOLIPIDS, PHOSPHORYLATION, ELECTROSPRAY IONIZATION, PHOSPHOTRANSFERASE, VEGETABLE PROTEIN, CHEMISTRY, ELECTROSPRAY MASS SPECTROMETRY, ENZYMOLOGY, EVALUATION, GROWTH, DEVELOPMENT AND AGING, LIPID METABOLISM, METHODOLOGY, PLANT TUBER, PHOSPHOTRANSFERASES, PLANT PROTEINS, PLANT TUBERS, SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION, |
| Online Access: | http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=46640 |
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