Activation of a calcium - dependent protein kinase involved in the Azospirillum growth promotion in rice

Rice seedlings (Oryza sativa) inoculated with the plant growth-promoting rhizobacteria Azospirillum brasilense FT326 showed an enhanced development of the root system 3 days after inoculation. Later on, a remarkable enlargement of shoots was also evident. An increase in the Ca2 plus sign dependent histone kinase activity was also detected as a result of inoculation. The biochemical characterization and Western blot analysis of the kinase strongly supports the hypothesis that it belongs to a member of the rice CDPK family. The fact that the amount of the protein did not change upon inoculation seems to indicate that a posttranslational activation is responsible for the change in the enzymatic activity. An in-gel kinase experiment identified a 46 kDa CDPK like protein kinase as a putative component of the signal transduction pathway triggered by Azospirillum inoculation. To our knowledge, this is the first report on the possible involvement of a Ca2 plus sign dependent protein kinase in promotion of rice plants growth by A. brasilense.

Bibliographic Details

Main Authors:	Ribaudo, Claudia Mónica, Curá, José Alfredo, Cantore, María L.
Format:	Texto biblioteca
Language:	eng
Subjects:	AZOSPIRILLUM, RICE, CDPK, LATERAL ROOTS, GROWTH PROMOTION,
Online Access:	http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=45775 http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber= http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
Tags:	Add Tag No Tags, Be the first to tag this record!