Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates
Variation of soil-microbial communities are good bioindicators of human impacts in soils, such as different soils management or contamination. Considering that traditional methods of isolation and taxonomic analysis do not consider the functionality of the microbial community, Community-Level Physiological Profiles (CLPP) emerged as a complementary methodology to study microbial communities. Several studies have shown that Biolog® EcoPlates® are very useful for determining physiological differences between communities from different samples. However, commercial microplates have some disadvantages which led us to the idea of replacing them by microplates prepared in the laboratory (Laboratory's). Here, we compared both types of microplates using soil samples from a bioremediation assay. We compared a) the average well color development for each sample, b) the averages of absorbance values for each type of microplate, c) Principal Components, and d) Shannon-Weaver's diversity index (H). Although Laboratory's showed significantly lower Average absorbance values than EcoPlates®, the principal component analysis and diversity index did not differ between types of microplates. In conclusion, both types of microplates showed a relatively similar ability to detect differences in the CLPP of the treatments studied. Consequently, microplates prepared in laboratory are a reliable and economical tool to study the physiology of soil microbial communities.
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Subjects: | CARBON-SOURCE UTILIZATION, COMMUNITY-LEVEL PHYSIOLOGICAL PROFILES, FUNCTIONAL DIVERSITY, METABOLIC PROFILES, ANTHROPOGENIC EFFECT, BIOASSAY, BIOINDICATOR, BIOREMEDIATION, DIVERSITY INDEX, FUNCTIONAL GROUP, LABORATORY METHOD, METABOLISM, MICROBIAL ACTIVITY, MICROBIAL COMMUNITY, NUTRIENT USE, SOIL CARBON, SOIL ECOSYSTEM, SOIL MICROORGANISM, STANDARDIZATION, TAXONOMY, |
Online Access: | http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012DiSalvo |
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snrd:2012DiSalvo2021-10-15T16:56:07Z Di Salvo, L. P. García de Salamone, I. E. 2012 Variation of soil-microbial communities are good bioindicators of human impacts in soils, such as different soils management or contamination. Considering that traditional methods of isolation and taxonomic analysis do not consider the functionality of the microbial community, Community-Level Physiological Profiles (CLPP) emerged as a complementary methodology to study microbial communities. Several studies have shown that Biolog® EcoPlates® are very useful for determining physiological differences between communities from different samples. However, commercial microplates have some disadvantages which led us to the idea of replacing them by microplates prepared in the laboratory (Laboratory's). Here, we compared both types of microplates using soil samples from a bioremediation assay. We compared a) the average well color development for each sample, b) the averages of absorbance values for each type of microplate, c) Principal Components, and d) Shannon-Weaver's diversity index (H). Although Laboratory's showed significantly lower Average absorbance values than EcoPlates®, the principal component analysis and diversity index did not differ between types of microplates. In conclusion, both types of microplates showed a relatively similar ability to detect differences in the CLPP of the treatments studied. Consequently, microplates prepared in laboratory are a reliable and economical tool to study the physiology of soil microbial communities. application/pdf 0327-5477 http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012DiSalvo eng info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 Ecología austral Vol.22, no.2 129-136 https://asaeargentina.com.ar CARBON-SOURCE UTILIZATION COMMUNITY-LEVEL PHYSIOLOGICAL PROFILES FUNCTIONAL DIVERSITY METABOLIC PROFILES ANTHROPOGENIC EFFECT BIOASSAY BIOINDICATOR BIOREMEDIATION DIVERSITY INDEX FUNCTIONAL GROUP LABORATORY METHOD METABOLISM MICROBIAL ACTIVITY MICROBIAL COMMUNITY NUTRIENT USE SOIL CARBON SOIL ECOSYSTEM SOIL MICROORGANISM STANDARDIZATION TAXONOMY Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates article info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion publishedVersion |
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CARBON-SOURCE UTILIZATION COMMUNITY-LEVEL PHYSIOLOGICAL PROFILES FUNCTIONAL DIVERSITY METABOLIC PROFILES ANTHROPOGENIC EFFECT BIOASSAY BIOINDICATOR BIOREMEDIATION DIVERSITY INDEX FUNCTIONAL GROUP LABORATORY METHOD METABOLISM MICROBIAL ACTIVITY MICROBIAL COMMUNITY NUTRIENT USE SOIL CARBON SOIL ECOSYSTEM SOIL MICROORGANISM STANDARDIZATION TAXONOMY CARBON-SOURCE UTILIZATION COMMUNITY-LEVEL PHYSIOLOGICAL PROFILES FUNCTIONAL DIVERSITY METABOLIC PROFILES ANTHROPOGENIC EFFECT BIOASSAY BIOINDICATOR BIOREMEDIATION DIVERSITY INDEX FUNCTIONAL GROUP LABORATORY METHOD METABOLISM MICROBIAL ACTIVITY MICROBIAL COMMUNITY NUTRIENT USE SOIL CARBON SOIL ECOSYSTEM SOIL MICROORGANISM STANDARDIZATION TAXONOMY |
spellingShingle |
CARBON-SOURCE UTILIZATION COMMUNITY-LEVEL PHYSIOLOGICAL PROFILES FUNCTIONAL DIVERSITY METABOLIC PROFILES ANTHROPOGENIC EFFECT BIOASSAY BIOINDICATOR BIOREMEDIATION DIVERSITY INDEX FUNCTIONAL GROUP LABORATORY METHOD METABOLISM MICROBIAL ACTIVITY MICROBIAL COMMUNITY NUTRIENT USE SOIL CARBON SOIL ECOSYSTEM SOIL MICROORGANISM STANDARDIZATION TAXONOMY CARBON-SOURCE UTILIZATION COMMUNITY-LEVEL PHYSIOLOGICAL PROFILES FUNCTIONAL DIVERSITY METABOLIC PROFILES ANTHROPOGENIC EFFECT BIOASSAY BIOINDICATOR BIOREMEDIATION DIVERSITY INDEX FUNCTIONAL GROUP LABORATORY METHOD METABOLISM MICROBIAL ACTIVITY MICROBIAL COMMUNITY NUTRIENT USE SOIL CARBON SOIL ECOSYSTEM SOIL MICROORGANISM STANDARDIZATION TAXONOMY Di Salvo, L. P. García de Salamone, I. E. Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates |
description |
Variation of soil-microbial communities are good bioindicators of human impacts in soils, such as different soils management or contamination. Considering that traditional methods of isolation and taxonomic analysis do not consider the functionality of the microbial community, Community-Level Physiological Profiles (CLPP) emerged as a complementary methodology to study microbial communities. Several studies have shown that Biolog® EcoPlates® are very useful for determining physiological differences between communities from different samples. However, commercial microplates have some disadvantages which led us to the idea of replacing them by microplates prepared in the laboratory (Laboratory's). Here, we compared both types of microplates using soil samples from a bioremediation assay. We compared a) the average well color development for each sample, b) the averages of absorbance values for each type of microplate, c) Principal Components, and d) Shannon-Weaver's diversity index (H). Although Laboratory's showed significantly lower Average absorbance values than EcoPlates®, the principal component analysis and diversity index did not differ between types of microplates. In conclusion, both types of microplates showed a relatively similar ability to detect differences in the CLPP of the treatments studied. Consequently, microplates prepared in laboratory are a reliable and economical tool to study the physiology of soil microbial communities. |
format |
article |
topic_facet |
CARBON-SOURCE UTILIZATION COMMUNITY-LEVEL PHYSIOLOGICAL PROFILES FUNCTIONAL DIVERSITY METABOLIC PROFILES ANTHROPOGENIC EFFECT BIOASSAY BIOINDICATOR BIOREMEDIATION DIVERSITY INDEX FUNCTIONAL GROUP LABORATORY METHOD METABOLISM MICROBIAL ACTIVITY MICROBIAL COMMUNITY NUTRIENT USE SOIL CARBON SOIL ECOSYSTEM SOIL MICROORGANISM STANDARDIZATION TAXONOMY |
author |
Di Salvo, L. P. García de Salamone, I. E. |
author_facet |
Di Salvo, L. P. García de Salamone, I. E. |
author_sort |
Di Salvo, L. P. |
title |
Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates |
title_short |
Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates |
title_full |
Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates |
title_fullStr |
Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates |
title_full_unstemmed |
Evaluation of soil-microbial communities by their CLPP : standardization of a laboratory technique to replace commercial available microplates |
title_sort |
evaluation of soil-microbial communities by their clpp : standardization of a laboratory technique to replace commercial available microplates |
url |
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012DiSalvo |
work_keys_str_mv |
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