Morphometric and molecular analysis (mtDNA) of honeybees (Apis mellifera L.) in the state of Tabasco, Mexico

Morphometric and molecular analysis (mtDNA) of honeybees (Apis mellifera L.) in the state of Tabasco, Mexico

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Beekeeping in Mexico is based on subspecies of European (Apis mellifera L) and Africanized bees. Due to the difficulty in the morphological differentiation of the European (E) and Africanized (A) populations of A. mellifera, the objective of this work was to perform a comparative analysis between the FABIS morphometric technique, against the diagnosis using the restriction fragment length polymorphism (RFLP) of mitochondrial DNA (mtDNA). Samples of bees from 135 commercial colonies (CC), 15 breeding colonies (BRC) and 3 wild colonies (WC), located in different municipalities of the state of Tabasco (N= 153), were used. Both diagnostic methods identified BRCs as European and WCs as Africanized, but in CCs, the FABIS method could not define 9 colonies, considering them as suspicious (S) and another 50 did not coincide with the result of the molecular method, so, in total, both methods coincided in 94 identifications (61.44 %). The Bayesian grouping based on the analysis of the twelve morphometric variables showed that the categories A-CC and E-CC form a group close to the category E-BRC; while the category A-WC presented the greatest distance, forming an isolated group. Therefore, CC bees have morphometric characteristics tending to E-BRC bees. This work is also intended to be a contribution to the lack of records on Africanization in the state of Tabasco. It is recommended to use the molecular method to discriminate between E/A bees, as it is not affected by environmental factors.

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Main Authors: Gómez Leyva, Juan Florencio, Argüello Nájera, Omar, Vázquez Encino, Pablo Jorge, Hernández Hernández, Luis Ulises, Payró de la Cruz, Emeterio
Format: Digital revista
Language:spa
eng
Published: Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias 2022
Online Access:https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5144
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id rev-remexcp-article5144
record_format ojs
institution INIFAP
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country México
countrycode MX
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databasecode rev-remexcp
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region America del Norte
libraryname Biblioteca INIFAP
language spa
eng
format Digital
author Gómez Leyva, Juan Florencio
Argüello Nájera, Omar
Vázquez Encino, Pablo Jorge
Hernández Hernández, Luis Ulises
Payró de la Cruz, Emeterio
spellingShingle Gómez Leyva, Juan Florencio
Argüello Nájera, Omar
Vázquez Encino, Pablo Jorge
Hernández Hernández, Luis Ulises
Payró de la Cruz, Emeterio
Morphometric and molecular analysis (mtDNA) of honeybees (Apis mellifera L.) in the state of Tabasco, Mexico
author_facet Gómez Leyva, Juan Florencio
Argüello Nájera, Omar
Vázquez Encino, Pablo Jorge
Hernández Hernández, Luis Ulises
Payró de la Cruz, Emeterio
author_sort Gómez Leyva, Juan Florencio
title Morphometric and molecular analysis (mtDNA) of honeybees (Apis mellifera L.) in the state of Tabasco, Mexico
title_short Morphometric and molecular analysis (mtDNA) of honeybees (Apis mellifera L.) in the state of Tabasco, Mexico
title_full Morphometric and molecular analysis (mtDNA) of honeybees (Apis mellifera L.) in the state of Tabasco, Mexico
title_fullStr Morphometric and molecular analysis (mtDNA) of honeybees (Apis mellifera L.) in the state of Tabasco, Mexico
title_full_unstemmed Morphometric and molecular analysis (mtDNA) of honeybees (Apis mellifera L.) in the state of Tabasco, Mexico
title_sort morphometric and molecular analysis (mtdna) of honeybees (apis mellifera l.) in the state of tabasco, mexico
description Beekeeping in Mexico is based on subspecies of European (Apis mellifera L) and Africanized bees. Due to the difficulty in the morphological differentiation of the European (E) and Africanized (A) populations of A. mellifera, the objective of this work was to perform a comparative analysis between the FABIS morphometric technique, against the diagnosis using the restriction fragment length polymorphism (RFLP) of mitochondrial DNA (mtDNA). Samples of bees from 135 commercial colonies (CC), 15 breeding colonies (BRC) and 3 wild colonies (WC), located in different municipalities of the state of Tabasco (N= 153), were used. Both diagnostic methods identified BRCs as European and WCs as Africanized, but in CCs, the FABIS method could not define 9 colonies, considering them as suspicious (S) and another 50 did not coincide with the result of the molecular method, so, in total, both methods coincided in 94 identifications (61.44 %). The Bayesian grouping based on the analysis of the twelve morphometric variables showed that the categories A-CC and E-CC form a group close to the category E-BRC; while the category A-WC presented the greatest distance, forming an isolated group. Therefore, CC bees have morphometric characteristics tending to E-BRC bees. This work is also intended to be a contribution to the lack of records on Africanization in the state of Tabasco. It is recommended to use the molecular method to discriminate between E/A bees, as it is not affected by environmental factors.
publisher Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
publishDate 2022
url https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5144
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spelling rev-remexcp-article51442022-02-11T17:23:56Z Morphometric and molecular analysis (mtDNA) of honeybees (Apis mellifera L.) in the state of Tabasco, Mexico Análisis morfométrico y molecular (ADNmt) de abejas melíferas (Apis mellifera L.) en el estado de Tabasco, México Gómez Leyva, Juan Florencio Argüello Nájera, Omar Vázquez Encino, Pablo Jorge Hernández Hernández, Luis Ulises Payró de la Cruz, Emeterio colonies; africanization; PCR; morphometry; mtDNA colonias; africanización; PCR; morfometría; ADNmt Beekeeping in Mexico is based on subspecies of European (Apis mellifera L) and Africanized bees. Due to the difficulty in the morphological differentiation of the European (E) and Africanized (A) populations of A. mellifera, the objective of this work was to perform a comparative analysis between the FABIS morphometric technique, against the diagnosis using the restriction fragment length polymorphism (RFLP) of mitochondrial DNA (mtDNA). Samples of bees from 135 commercial colonies (CC), 15 breeding colonies (BRC) and 3 wild colonies (WC), located in different municipalities of the state of Tabasco (N= 153), were used. Both diagnostic methods identified BRCs as European and WCs as Africanized, but in CCs, the FABIS method could not define 9 colonies, considering them as suspicious (S) and another 50 did not coincide with the result of the molecular method, so, in total, both methods coincided in 94 identifications (61.44 %). The Bayesian grouping based on the analysis of the twelve morphometric variables showed that the categories A-CC and E-CC form a group close to the category E-BRC; while the category A-WC presented the greatest distance, forming an isolated group. Therefore, CC bees have morphometric characteristics tending to E-BRC bees. This work is also intended to be a contribution to the lack of records on Africanization in the state of Tabasco. It is recommended to use the molecular method to discriminate between E/A bees, as it is not affected by environmental factors. La apicultura en México se sostiene con subespecies de abejas (Apis mellifera L) europeas y africanizadas. Debido a la dificultad en la diferenciación morfológica de las poblaciones Europeas (E) y Africanizadas (A) de A. mellifera, el objetivo de este trabajo fue realizar un análisis comparativo entre la técnica morfométrica FABIS, contra el diagnóstico empleando el polimorfismo en la longitud de los fragmentos de restricción (RFLP) del ADN mitocondrial (ADNmt). Se emplearon muestras de abejas procedentes de 135 colonias comerciales (CC), 15 colonias pie de cría (CPC) y 3 colonias silvestres (CS) localizados en diferentes municipios del estado de Tabasco (N=153). Ambos métodos de diagnóstico, identificaron a las CPC como europeas y las CS como africanizadas, pero en las CC el método FABIS no pudo definir 9 colonias, dándolas como sospechosas (S) y otras 50 no coincidieron con el resultado del método molecular, por lo que, en total ambos métodos coincidieron en 94 identificaciones (61.44 %). El agrupamiento bayesiano basado en el análisis de las doce variables morfométricas mostró que las categorías A-CC y E-CC, forman un grupo cercano a la categoría E-CPC; mientras que la categoría A-CS presentó la mayor distancia formando un grupo aislado. Por lo tanto, abejas de CC tienen características morfométricas tendientes a abejas E-CPC. Este trabajo también pretende ser una contribución a la falta de registros sobre la africanización en el estado de Tabasco. Se recomienda emplear el método molecular para discriminar entre abejas E/A, por no estar afectado por los factores ambientales. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias TECNOLÓGICO NACIONAL DE MÉXICO 2022-02-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf application/pdf https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5144 10.22319/rmcp.v12i4.5144 Revista Mexicana de Ciencias Pecuarias; Vol. 12, Núm. 4 (2021): Octubre-Diciembre; 1188-1207 Revista Mexicana de Ciencias Pecuarias; Vol. 12, Núm. 4 (2021): Octubre-Diciembre; 1188-1207 2448-6698 2007-1124 spa eng https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5144/4669 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5144/4670 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/downloadSuppFile/5144/1227 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/downloadSuppFile/5144/2341 http://creativecommons.org/licenses/by-nc-sa/4.0

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