Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm
The objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated to the pathogenicity against S. frugiperda, explaining up to 67% of the phenotypic variation. Besides identification and molecular characterization of Beauveria isolates, ITS sequence and RAPD markers proved to be very useful in selecting the isolates potentially effective against S. frugiperda larvae and in monitoring field release of these microorganisms in biocontrol programs.
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Pesquisa Agropecuaria Brasileira
2008
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Carneiro, Andréa Almeida Gomes, Eliane Aparecida Guimarães, Claudia Teixeira Fernandes, Fernando Tavares Carneiro, Newton Portilho Cruz, Ivan |
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Carneiro, Andréa Almeida Gomes, Eliane Aparecida Guimarães, Claudia Teixeira Fernandes, Fernando Tavares Carneiro, Newton Portilho Cruz, Ivan Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm |
| author_facet |
Carneiro, Andréa Almeida Gomes, Eliane Aparecida Guimarães, Claudia Teixeira Fernandes, Fernando Tavares Carneiro, Newton Portilho Cruz, Ivan |
| author_sort |
Carneiro, Andréa Almeida |
| title |
Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm |
| title_short |
Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm |
| title_full |
Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm |
| title_fullStr |
Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm |
| title_full_unstemmed |
Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm |
| title_sort |
molecular characterization and pathogenicity of isolates of beauveria spp. to fall armyworm |
| description |
The objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated to the pathogenicity against S. frugiperda, explaining up to 67% of the phenotypic variation. Besides identification and molecular characterization of Beauveria isolates, ITS sequence and RAPD markers proved to be very useful in selecting the isolates potentially effective against S. frugiperda larvae and in monitoring field release of these microorganisms in biocontrol programs. |
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Pesquisa Agropecuaria Brasileira |
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2008 |
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https://seer.sct.embrapa.br/index.php/pab/article/view/81 |
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rev-pab-br-article-812014-05-16T17:40:43Z Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm Genotipaje de aislados de Beauveria spp. por secuenciamiento de la región ITS del rDNA y marcadores RAPD Caracterização molecular e virulência de isolados de Beauveria spp. contra a lagarta-do-cartucho Carneiro, Andréa Almeida Gomes, Eliane Aparecida Guimarães, Claudia Teixeira Fernandes, Fernando Tavares Carneiro, Newton Portilho Cruz, Ivan Spodoptera frugiperda; entomopathogenic fungi; RAPD; rDNA-ITS Spodoptera frugiperda;fungo entomopatogênico;RAPD;rDNA-ITS The objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated to the pathogenicity against S. frugiperda, explaining up to 67% of the phenotypic variation. Besides identification and molecular characterization of Beauveria isolates, ITS sequence and RAPD markers proved to be very useful in selecting the isolates potentially effective against S. frugiperda larvae and in monitoring field release of these microorganisms in biocontrol programs. Beauveria es un hongo deuteromiceto de gran espectro entomopatogénico. Existe mucho interés en el uso de ese hongo en programas integrados de manejo de plagas, como una alternativa a los defensivos químicos. La caracterización molecular de los aislados altamente virulentos es esencial en la selección de potenciales candidatos para utilización en control biológico. Se han evaluado en este trabajo 24 aislados de Beauveria, en bioensayos contra gusanos de Spodoptera frugiperda y molecularmente caracterizados por secuenciamiento de la región ITS del rDNA y marcadores RAPD. El secuenciamiento de fragmentos de 570 pares de bases de la región ITS del rDNA posibilitó la identificación de los aislados como B. bassiana y B. brongniarti, por la comparación con secuencias depositadas en el GenBank, pero no fue suficiente para discriminar los aislados dentro de cada especie. Sin embargo, 67 fragmentos polimórficos de RAPD fueron capaces de diferenciar casi todos los aislados de Beauveria, agrupándolos de acuerdo con el insecto hospedero y la virulencia contra el gusano cogollero del maíz. Los marcadores RAPD fueron altamente asociados a la virulencia contra S. frugiperda, explicando hasta el 67% de la variación fenotípica. Por lo tanto, además de la identificación y caracterización molecular de aislados de Beauveria, el secuenciamiento de la región ITS y los marcadores RAPD fueron útiles en la selección de aislados potencialmente eficientes contra gusanos de S. frugiperda e en el monitoramiento de liberaciones de esos microrganismos, en programas de biocontrol O objetivo deste trabalho foi avaliar a patogenicidade de 24 isolados de Beauveria contra larvas de Spodoptera frugiperda e caracterizá-los molecularmente, por meio do seqüenciamento da região ITS do rDNA e de marcadores RAPD. O seqüenciamento de fragmentos de 570 pares de bases, da região ITS do rDNA, possibilitou a identificação dos isolados como B. bassiana e B. brongniarti, pela comparação com seqüências depositadas no GenBank. Sessenta e sete fragmentos polimórficos de RAPD foram capazes de diferenciar 20 entre 24 isolados de Beauveria, e agrupá-los de acordo com o inseto hospedeiro e com a patogenicidade contra a lagarta-do-cartucho do milho. Os marcadores RAPD foram altamente associados à patogenicidade contra S. frugiperda e explicaram até 67% da variação fenotípica. Além da identificação e caracterização molecular de isolados de Beauveria, o seqüenciamento da região ITS, aliado aos marcadores RAPD, é útil na seleção de isolados potencialmente eficazes contra larvas de S. frugiperda e no monitoramento de liberações desses microrganismos em programas de biocontrole. Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira Embrapa 2008-10-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://seer.sct.embrapa.br/index.php/pab/article/view/81 Pesquisa Agropecuaria Brasileira; v.43, n.4, abr. 2008; 513-520 Pesquisa Agropecuária Brasileira; v.43, n.4, abr. 2008; 513-520 1678-3921 0100-104x por https://seer.sct.embrapa.br/index.php/pab/article/view/81/5415 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/81/455 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/81/456 |