Implications of interaction effects on the interpretation of factorial experiments in biology

Factorial experiments are commonly used in biology, particularly to study the way in which two or more factors are related. The presence of a significant interaction indicates that the factors are not independent. In this case, two related aspects should be considered: 1) the post hoc analysis after detecting the significant interaction, and 2) the conclusion on the other hypotheses included in the model. The objective of this work is to discuss the importance and implicances of the significant interactions on the inferences of the hypotheses of factorial models with fixed effects. We include four hypothetical problems in ecology with hypothetical data reflecting different scenarios with significant interactions. In each example, we discuss the possible biological significance of the marginal means of one factor averaged over the levels of the other in the presence of significant interactions, their post hoc analysis, and inferences.

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Main Authors: Willems, Priscila, Raffaele, Estela
Format: Digital revista
Language:spa
Published: Asociación Argentina de Ecología 2001
Online Access:https://ojs.ecologiaaustral.com.ar/index.php/Ecologia_Austral/article/view/1578
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spelling rev-ecoaus-article-15782021-01-13T04:01:03Z Implications of interaction effects on the interpretation of factorial experiments in biology Implicancias del efecto de las interacciones en la interpretación de experimentos factoriales en biología Willems, Priscila Raffaele, Estela Factorial experiments are commonly used in biology, particularly to study the way in which two or more factors are related. The presence of a significant interaction indicates that the factors are not independent. In this case, two related aspects should be considered: 1) the post hoc analysis after detecting the significant interaction, and 2) the conclusion on the other hypotheses included in the model. The objective of this work is to discuss the importance and implicances of the significant interactions on the inferences of the hypotheses of factorial models with fixed effects. We include four hypothetical problems in ecology with hypothetical data reflecting different scenarios with significant interactions. In each example, we discuss the possible biological significance of the marginal means of one factor averaged over the levels of the other in the presence of significant interactions, their post hoc analysis, and inferences. Los experimentos factoriales son muy usados en biología, especialmente para estudiar la forma en que dos o más factores se relacionan. La presencia de una interacción significativa indica que los factores no son independientes entre sí. En experimentos factoriales de efectos fijos hay dos aspectos relacionados a considerar frente a una interacción significativa: 1) el análisis posterior una vez detectada la misma, y 2) la conclusión sobre el resto de las hipótesis del modelo. El objetivo de este trabajo es discutir las implicancias que tienen las interacciones significativas sobre las inferencias a realizar en el conjunto de hipótesis de experimentos factoriales de efectos fijos. Se analizan datos correspondientes a cuatro experimentos ecológicos hipotéticos en los cuales la interacción entre los factores es significativa. De cada uno de los ejemplos se discute la interpretación biológica de los promedios marginales ante la existencia de la interacción. Asociación Argentina de Ecología 2001-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Ayuda didáctica application/pdf https://ojs.ecologiaaustral.com.ar/index.php/Ecologia_Austral/article/view/1578 Ecología Austral; Vol. 11 No. 2 (2001); 131-138 Ecología Austral; Vol. 11 Núm. 2 (2001); 131-138 0327-5477 1667-7838 spa https://ojs.ecologiaaustral.com.ar/index.php/Ecologia_Austral/article/view/1578/912 Derechos de autor 2021 Ecología Austral
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country Argentina
countrycode AR
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databasecode rev-ecoaus
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libraryname Asociación Argentina de Ecología
language spa
format Digital
author Willems, Priscila
Raffaele, Estela
spellingShingle Willems, Priscila
Raffaele, Estela
Implications of interaction effects on the interpretation of factorial experiments in biology
author_facet Willems, Priscila
Raffaele, Estela
author_sort Willems, Priscila
title Implications of interaction effects on the interpretation of factorial experiments in biology
title_short Implications of interaction effects on the interpretation of factorial experiments in biology
title_full Implications of interaction effects on the interpretation of factorial experiments in biology
title_fullStr Implications of interaction effects on the interpretation of factorial experiments in biology
title_full_unstemmed Implications of interaction effects on the interpretation of factorial experiments in biology
title_sort implications of interaction effects on the interpretation of factorial experiments in biology
description Factorial experiments are commonly used in biology, particularly to study the way in which two or more factors are related. The presence of a significant interaction indicates that the factors are not independent. In this case, two related aspects should be considered: 1) the post hoc analysis after detecting the significant interaction, and 2) the conclusion on the other hypotheses included in the model. The objective of this work is to discuss the importance and implicances of the significant interactions on the inferences of the hypotheses of factorial models with fixed effects. We include four hypothetical problems in ecology with hypothetical data reflecting different scenarios with significant interactions. In each example, we discuss the possible biological significance of the marginal means of one factor averaged over the levels of the other in the presence of significant interactions, their post hoc analysis, and inferences.
publisher Asociación Argentina de Ecología
publishDate 2001
url https://ojs.ecologiaaustral.com.ar/index.php/Ecologia_Austral/article/view/1578
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AT willemspriscila implicanciasdelefectodelasinteraccionesenlainterpretaciondeexperimentosfactorialesenbiologia
AT raffaeleestela implicanciasdelefectodelasinteraccionesenlainterpretaciondeexperimentosfactorialesenbiologia
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