Development and comparison of two validated methods to determine cadmium and arsenic in Colombian rice by ICP-MS and ICP-OES
Some metals are essential for the diet, such as iron (Fe), copper (Cu), manganese (Mn), and zinc (Zn). However, there are heavy metals that are not biodegradable, so they are not essential for metabolism since they do not fulfill a physiological role in humans or plants. Therefore, they become an adverse factor in terms of health due to their cumulative capacities, such as cadmium (Cd), chromium (Cr), lead (Pb), arsenic (As), and mercury (Hg). This study aimed to develop and compare two methods to determine the content of Cd and As in rice grains (Oryza sativa L.). The samples were subjected to microwave-assisted acid digestion with HNO3, and H2O2 [2:1] as this method is useful to prepare samples with the presence of organic matter since it minimizes the loss of analytes and sample contamination. Subsequently, these were analyzed by quadrupole inductively coupled plasma-mass spectrometry (ICP-MS) equipped with a collision/reaction cell and by inductively coupled plasma-optical emission spectrometry (ICP-OES). The performance of the proposed method was evaluated under selectivity, sensitivity, linear range, limits of detection and quantification, precision, intermediate precision, and reproducibility parameters. The results showed that the digestion method was satisfactory for sample preparation and the ICP-MS technique obtained lower quantification limits compared to ICP-OES. However, due to the quantified concentrations, the results indicated that both methods are suitable to determine Cd and As in rice, considering the maximum limits allowed in different types of rice regulated in the Codex Alimentarius and the European Union.
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Universidad del Cauca -Facultad de ciencias Agrarias
2024
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Rodríguez Giraldo, Yeni Rodriguez Sánchez, Estefania Cifuentes Muñoz, Edwin Alfredo Martínez Hernandez, Yuri Pichimata Sanabria, María Angélica |
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Rodríguez Giraldo, Yeni Rodriguez Sánchez, Estefania Cifuentes Muñoz, Edwin Alfredo Martínez Hernandez, Yuri Pichimata Sanabria, María Angélica Development and comparison of two validated methods to determine cadmium and arsenic in Colombian rice by ICP-MS and ICP-OES |
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Rodríguez Giraldo, Yeni Rodriguez Sánchez, Estefania Cifuentes Muñoz, Edwin Alfredo Martínez Hernandez, Yuri Pichimata Sanabria, María Angélica |
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Rodríguez Giraldo, Yeni |
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Development and comparison of two validated methods to determine cadmium and arsenic in Colombian rice by ICP-MS and ICP-OES |
| title_short |
Development and comparison of two validated methods to determine cadmium and arsenic in Colombian rice by ICP-MS and ICP-OES |
| title_full |
Development and comparison of two validated methods to determine cadmium and arsenic in Colombian rice by ICP-MS and ICP-OES |
| title_fullStr |
Development and comparison of two validated methods to determine cadmium and arsenic in Colombian rice by ICP-MS and ICP-OES |
| title_full_unstemmed |
Development and comparison of two validated methods to determine cadmium and arsenic in Colombian rice by ICP-MS and ICP-OES |
| title_sort |
development and comparison of two validated methods to determine cadmium and arsenic in colombian rice by icp-ms and icp-oes |
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Some metals are essential for the diet, such as iron (Fe), copper (Cu), manganese (Mn), and zinc (Zn). However, there are heavy metals that are not biodegradable, so they are not essential for metabolism since they do not fulfill a physiological role in humans or plants. Therefore, they become an adverse factor in terms of health due to their cumulative capacities, such as cadmium (Cd), chromium (Cr), lead (Pb), arsenic (As), and mercury (Hg). This study aimed to develop and compare two methods to determine the content of Cd and As in rice grains (Oryza sativa L.). The samples were subjected to microwave-assisted acid digestion with HNO3, and H2O2 [2:1] as this method is useful to prepare samples with the presence of organic matter since it minimizes the loss of analytes and sample contamination. Subsequently, these were analyzed by quadrupole inductively coupled plasma-mass spectrometry (ICP-MS) equipped with a collision/reaction cell and by inductively coupled plasma-optical emission spectrometry (ICP-OES). The performance of the proposed method was evaluated under selectivity, sensitivity, linear range, limits of detection and quantification, precision, intermediate precision, and reproducibility parameters. The results showed that the digestion method was satisfactory for sample preparation and the ICP-MS technique obtained lower quantification limits compared to ICP-OES. However, due to the quantified concentrations, the results indicated that both methods are suitable to determine Cd and As in rice, considering the maximum limits allowed in different types of rice regulated in the Codex Alimentarius and the European Union. |
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Universidad del Cauca -Facultad de ciencias Agrarias |
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2024 |
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https://revistas.unicauca.edu.co/index.php/biotecnologia/article/view/2345 |
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rev-bsaa-co-article-23452024-03-04T17:06:16Z Development and comparison of two validated methods to determine cadmium and arsenic in Colombian rice by ICP-MS and ICP-OES Desarrollo y comparación de dos métodos validados para determinar cadmio y arsénico en arroz colombiano mediante ICP-MS e ICP-OES Rodríguez Giraldo, Yeni Rodriguez Sánchez, Estefania Cifuentes Muñoz, Edwin Alfredo Martínez Hernandez, Yuri Pichimata Sanabria, María Angélica Heavy metals Validation Microwave-assisted acid digestion Quantification limits Food Metales pesados Validación Digestión ácida asistida por microondas Límites de cuantificación Alimentos Some metals are essential for the diet, such as iron (Fe), copper (Cu), manganese (Mn), and zinc (Zn). However, there are heavy metals that are not biodegradable, so they are not essential for metabolism since they do not fulfill a physiological role in humans or plants. Therefore, they become an adverse factor in terms of health due to their cumulative capacities, such as cadmium (Cd), chromium (Cr), lead (Pb), arsenic (As), and mercury (Hg). This study aimed to develop and compare two methods to determine the content of Cd and As in rice grains (Oryza sativa L.). The samples were subjected to microwave-assisted acid digestion with HNO3, and H2O2 [2:1] as this method is useful to prepare samples with the presence of organic matter since it minimizes the loss of analytes and sample contamination. Subsequently, these were analyzed by quadrupole inductively coupled plasma-mass spectrometry (ICP-MS) equipped with a collision/reaction cell and by inductively coupled plasma-optical emission spectrometry (ICP-OES). The performance of the proposed method was evaluated under selectivity, sensitivity, linear range, limits of detection and quantification, precision, intermediate precision, and reproducibility parameters. The results showed that the digestion method was satisfactory for sample preparation and the ICP-MS technique obtained lower quantification limits compared to ICP-OES. However, due to the quantified concentrations, the results indicated that both methods are suitable to determine Cd and As in rice, considering the maximum limits allowed in different types of rice regulated in the Codex Alimentarius and the European Union. Algunos metales son esenciales para la dieta, como el hierro (Fe), el cobre (Cu), el manganeso (Mn) y el zinc (Zn). Sin embargo, hay metales pesados que no son biodegradables, por lo que no son esenciales para el metabolismo ya que no cumplen un papel fisiológico en los seres humanos ni en las plantas. Por ello, se convierten en un factor adverso para la salud por sus capacidades acumulativas, como el cadmio (Cd), el cromo (Cr), el plomo (Pb), el arsénico (As) y el mercurio (Hg). Este estudio tuvo como objetivo desarrollar y comparar dos métodos para determinar el contenido de Cd y As en granos de arroz (Oryza sativa L.). Las muestras se sometieron a digestión ácida asistida por microondas con HNO3, y H2O2 [2:1] ya que este método es útil para preparar muestras con presencia de materia orgánica puesto que minimiza la pérdida de analitos y la contaminación de la muestra. Posteriormente, se analizaron mediante espectrometría de masas con plasma de acoplamiento inductivo cuadrupolar (ICP-MS) equipada con una célula de colisión/reacción y mediante espectrometría de emisión óptica con plasma de acoplamiento inductivo (ICP-OES). El rendimiento del método propuesto se evaluó según los parámetros de selectividad, sensibilidad, rango lineal, límites de detección y cuantificación, precisión, precisión intermedia y reproducibilidad. Los resultados mostraron que el método de digestión fue satisfactorio para la preparación de la muestra y que la técnica ICP-MS obtuvo límites de cuantificación más bajos en comparación con ICP-OES. Sin embargo, debido a las concentraciones cuantificadas, los resultados indicaron que ambos métodos son adecuados para determinar Cd y As en arroz, considerando los límites máximos permitidos en diferentes tipos de arroz regulados en el Codex Alimentarius y la Unión Europea Universidad del Cauca -Facultad de ciencias Agrarias 2024-03-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unicauca.edu.co/index.php/biotecnologia/article/view/2345 Biotechnology in the Agricultural and Agroindustrial Sector; Early Edition Biotecnología en el Sector Agropecuario y Agroindustrial; Edición Anticipada 1909-9959 1692-3561 spa https://revistas.unicauca.edu.co/index.php/biotecnologia/article/view/2345/1917 /*ref*/AL-NAIMI, MAHA; AL-GHOUTI, MOHAMMAD. Effects of Soaking, Acidity and Temperature on Cadmium and Lead Removal from Rice. Food Chemistry, v. 310, 2019, 125591. https://doi.org/10.1016/j.foodchem.2019.125591 /*ref*/ASTOLFI, MARIA-LUISA; MARCONI, ELISABETTA; PROTANO, CARMELA; VITALI, MATTEO; SCHIAVI, ELISA; MASTROMARINO, PAOLA; CANEPARI, SILVIA. Optimization and Validation of a Fast Digestion Method for the Determination of Major and Trace Elements in Breast Milk by ICP-MS. Analytica Chimica Acta, v. 1040, 2018, p. 49–62. https://doi.org/10.1016/j.aca.2018.07.037 /*ref*/BARI, MD-AZIZUL; AKTHER, MST-SALMA; REZA, MD-ABU; KABIR, AHMAD-HUMAYAN-. Cadmium Tolerance Is Associated with the Root-Driven Coordination of Cadmium Sequestration, Iron Regulation, and ROS Scavenging in Rice. Plant Physiology and Biochemistry, v. 136, 2019, p. 22–33. https://doi.org/10.1016/j.plaphy.2019.01.007 /*ref*/BASNET, PRIYANKA; AMARASIRIWARDENA, DULASIRI; WU, FENGCHANG; FU, ZHIYOU; ZHANG, TAO. Elemental Bioimaging of Tissue Level Trace Metal Distributions in Rice Seeds (Oryza Sativa L.) from a Mining Area in China. Environmental pollution, v. 195, 2014, p. 148–156. http://dx.doi.org/10.1016/j.envpol.2014.08.017 /*ref*/BRIFFA, JESSICA; SINAGRA, EMMANUEL; BLUNDELL, RENALD. Heavy Metal Pollution in the Environment and Their Toxicological Effects on Humans. Heliyon, v. 6, n. 9, 2020, e04691. http://dx.doi.org/10.1016/j.heliyon.2020.e04691 /*ref*/CAO, YINI; MA, CHUANXIN; YU, HAO; TAN, QIAN; DHANKHER, OM-PARKASH; WHITE, JASON; XING, BAOSHAN. The role of sulfur nutrition in plant response to metal(loid) stress: Facilitating biofortification and phytoremediation. Journal of Hazardous Materials, v. 443, Part B, 2023, 130283.https://doi.org/10.1016/j.jhazmat.2022.130283. /*ref*/CLEMENS, STEPHAN; AARTS, MARK; THOMINE, SÉBASTIEN; VERBRUGGEN, NATHALIE. Plant Science: The Key to Preventing Slow Cadmium Poisoning. Trends in Plant Science, v. 18, n. 2, 2013, p. 92–99.https://doi.org/10.1016/j.tplants.2012.08.003 /*ref*/DA SILVA, IAGO; LAVORANTE, ANDRÉ; PAIM, ANA P.S.; DA SILVA, MARIA J. Microwave-Assisted Digestion Employing Diluted Nitric Acid for Mineral Determination in Rice by ICP OES. Food Chemistry, 319, 2020, 126435.https://doi.org/10.1016/j.foodchem.2020.126435 /*ref*/DRAVA, GIULIANA; VINCENZO MINGANTI. Influence of an Internal Standard in Axial ICP OES Analysis of Trace Elements in Plant Materials. Journal of Analytical Atomic Spectrometry, v. 35, n. 2, 2020. p. 301–306. https://doi.org/10.1039/c9ja00372j /*ref*/MAGNUSSON, B.; ÖRNEMARK, U. Eurachem Guide: The Fitness for Purpose of Analytical Methods – A Laboratory Guide to Method Validation and Related Topics. 2 ed, 2014, ISBN 978-91-87461-59-0 /*ref*/FAKHAR, ALI; GUL, BUSHRA; GURMANI, ALI-RAZA; KHAN, SHAH-MASAUD; ALI, SHAFAQAT; SULTAN, TARIQ; CHAUDHARY, HASSAN-JAVED; RAFIQUE, MAZHAR; RIZWAN, MUHAMMAD. Heavy Metal Remediation and Resistance Mechanism of Aeromonas, Bacillus, and Pseudomonas: A Review. Critical Reviews in Environmental Science and Technology, v. 52, n. 11, 2022, p. 1868–1914.https://doi.org/10.1080/10643389.2020.1863112 /*ref*/FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS AND WORLD HEALTH ORGANIZATION (FAO and WHO). Codex Alimentarius, Rome, 2019. https://bit.ly/3bKpEEE /*ref*/GALLEGO, SUSANA M.; PENA, LILIANA B.; BARCIA, ROBERTO A.; AZPILICUETA, CLAUDIA E.; IANNONE, MARÍA F.; ROSALES, ELIANA P.; ZAWOZNIK, MYRIAM S.; GROPPA, MARÍA D.; BENAVIDES, MARÍA P. Unravelling Cadmium Toxicity and Tolerance in Plants: Insight into Regulatory Mechanisms. Environmental and Experimental Botany, v. 83, 2012, p. 33–46.http://dx.doi.org/10.1016/j.envexpbot.2012.04.006 /*ref*/GONÇALVES, DANIEL A.; DE SOUZA, IGOR-DOMINGOS; GOMES-ROSA, ANA-CARLA; PÁDUA-MELO, ELAINE-SILVA; GONCALVES, ALEM-MAR; DE OLIVEIRA, LINCOLN-CARLOS; DO NASCIMENTO, VALTER. Multi-Wavelength Calibration: Determination of Trace Toxic Elements in Medicine Plants by ICP OES. Microchemical Journal, v. 146, 2019, p. 381–386.https://doi.org/10.1016/j.microc.2019.01.021 /*ref*/HERRERO-FERNÁNDEZ, ZAHILY; ESTEVEZ-ÁLVAREZ, JUAN-REINALDO; MONTERO-ÁLVAREZ, ALFREDO; MUÑIZ-UGARTE, OLEGARIO; PUPO-GONZÁLEZ, IVÁN; RODRÍGUEZ-GONZÁLEZ, MAYDEL; DOS SANTOS-JÚNIOR, JOSÉ-ARAÚJO; CAVALCANTI-FREIRE-BEZERRA, MARIANA-BRAYNER; DOS SANTOS-JUNIOR, OTAVIO-PEREIRAL. Metal Contaminants in Rice from Cuba Analyzed by ICP-MS, ICP-AES and CVAAS. Food Additives and Contaminants: Part B Surveillance, v. 14, n. 1, 2021, p. 59–65.https://doi.org/10.1080/19393210.2020.1870576 /*ref*/INSTITUTO COLOMBIANO DE NORMAS TECNICAS Y CERTIFICACIÓN (ICONTEC). GTC-ISO-IEC 99. Vocabulario Internacional de Metrología. Conceptos Fundamentales, Generales y Términos Asociados (VIM). Bogotá (Colombia): v. 48, 2019. /*ref*/LEE, JIAN; PARK, YE-SEUL; LEE, HWA-JEONG; KOO, YONG-EUI. Microwave-Assisted Digestion Method Using Diluted Nitric Acid and Hydrogen Peroxide for the Determination of Major and Minor Elements in Milk Samples by ICP-OES and ICP-MS. Food Chemistry, v. 373, 2022,131483.https://doi.org/10.1016/j.foodchem.2021.131483 /*ref*/MEERMANN, BJÖRN; NISCHWITZ, VOLKER. ICP-MS for the Analysis at the Nanoscale-a Tutorial Review. Journal of Analytical Atomic Spectrometry, v. 33, n. 9, 2018, p. 1432–1468. https://doi.org/10.1039/c8ja00037a /*ref*/MUHAMMAD, ZAHIR; ABDUL, MAJEED; REHMANULLAH; NAILA, INAYAT; SAIRA, SIYAR. Effect of Heavy Metal Stress on Growth and Yields of Crop Plants: An Overview. In Faisal, M.; Saquib, Q.; Alatar, A.A.; Al-Khedhairy, A.A; Cellular and Molecular Phytotoxicity of Heavy Metals. Nanotechnology in the Life Sciences. United States of America: Springer, Cham, 2020, 451p. https://doi.org/10.1007/978-3-030-45975-8_4 /*ref*/NARUKAWA, T.; SHIKINO, O.; CHIBA, K. Application of Neon as a Collision Gas-a Cell Gas Index for Collision/Reaction Cell-ICP-MS Determination. Journal of Analytical Atomic Spectrometry, v. 35, n. 10, 2020, p. 2142–47. https://doi.org/10.1039/d0ja00176g /*ref*/ONDŘEJ, ZVĚŘINA; MONIKA, VYCHYTILOVÁ; JAQUELINE, RIEGER; WALTER, GOESSLER. Fast and Simultaneous Determination of Zinc and Iron Using HR-CS GF-AAS in Vegetables and Plant Material. Spectrochimica Acta Part B: Atomic Spectroscopy, v. 201, 2023, 106616.http://dx.doi.org/10.1016/j.sab.2023.106616 /*ref*/RAI, PRABHAT-KUMAR; LEE, SANG-SOO; ZHANG, MING; TSANG, YIU-FAI; KIM, KI-HYUNL. Heavy Metals in Food Crops: Health Risks, Fate, Mechanisms, and Management. Environment International, v. 125, 2019, p. 365–385.https://doi.org/10.1016/j.envint.2019.01.067 /*ref*/RAPOSO, FRANCISCO. Evaluation of Analytical Calibration Based on Least-Squares Linear Regression for Instrumental Techniques: A Tutorial Review. TrAC - Trends in Analytical Chemistry, v. 77, 2016, p. 167–185. http://dx.doi.org/10.1016/j.trac.2015.12.006 /*ref*/RAQUEL, SERRANO; GRINDLAY, GUILLERMO; GRAS, LUIS; MORA, JUAN. Insight into the Origin of Carbon Matrix Effects on the Emission Signal of Atomic Lines in Inductively Coupled Plasma Optical Emission Spectrometry. Spectrochimica Acta Part B: Atomic Spectroscopy, v. 177, 2021,106070. http://dx.doi.org/10.1016/j.sab.2021.106070 /*ref*/ROA-ACOSTA, DIEGO-FERNANDO; HOYOS-CONCHA, JOSÉ-LUIS; BRAVO-GOMEZ, JESÚS-EDUARDO. Hydrolysis Monitoring of Quinoa, Soy and Rice Proteins by Using Spectroscopy FT-IR Technique. Biotecnología en el Sector Agropecuario y Agroindustrial, v. 20, n. 2, 2022, p. 76-86.https://doi.org/10.18684/rbsaa.v20.n2.2022.1941 /*ref*/RODRÍGUEZ-GIRALDO, YENI; RODRIGUEZ-SÁNCHEZ, ESTEFANIA; TORRES, LUIS-GABRIEL; MONTENEGRO, ANDREA C.; PICHIMATA, MARÍA-ANGÉLICA. Development of Validation Methods to Determine Cadmium in Cocoa Almond from the Beans by ICP-MS and ICP-OES. Talanta Open, v. 5, 2022, 100078. https://doi.org/10.1016/j.talo.2021.100078 /*ref*/SANDEEP, G.; VIJAYALATHA, K.R.; ANITHA, T. Heavy Metals and Its Impact in Vegetable Crops. International Journal of Chemical Studies, v. 7, n. 1, 2019, p. 1612–1621. /*ref*/SEN, SAIKAT; CHAKRABORTY, RAJA; KALITA, PRATAP. Rice - Not Just a Staple Food: A Comprehensive Review on Its Phytochemicals and Therapeutic Potential. Trends in Food Science and Technology, v. 97, 2020, p. 265–85.https://doi.org/10.1016/j.tifs.2020.01.022 /*ref*/SPANU, ANTONINO; VALENTE, MASSIMILIANO; LANGASCO, ILARIA; LEARDI, RICCARDO; ORLANDONI, ANNA-MARIA; CIULU, MARCO; DEROMA, MARIO-ANTONELLO; SPANO, NADIA; BARRACU, FRANCESCO; PILO, MARIA-I; SANNA, GAVINO. Effect of the Irrigation Method and Genotype on the Bioaccumulation of Toxic and Trace Elements in Rice. Science of the Total Environment, v. 748, 2020, 142484.https://doi.org/10.1016/j.scitotenv.2020.142484 /*ref*/SUGIYAMA, NAOKI. Attenuation of Doubly Charged Ion Interferences on Arsenic and Selenium by ICP-MS under Low Kinetic Energy Collision Cell Conditions with Hydrogen Cell Gas. Journal of Analytical Atomic Spectrometry, v. 36, n. 2, 2021, p. 294–302.https://doi.org/10.1039/D0JA00301H /*ref*/TANASEA, ION-GH; POPAA, DANA-ELENA; UDRIŞTIOIU, GABRIELA-ELENA; BUNACIU, ANDREI A.; ABOUL‐ENEIN, HASSAN Y. Validation and Quality Control of an ICP‐MS Method for the Quantification and Discrimination of Trace Metals and Application in Paper Analysis: An Overview. Critical Reviews in Analytical Chemistry, v. 44, n. 4, 2014.https://doi.org/10.1080/10408347.2013.863141 /*ref*/TCHOUNWOU, PAUL B.; YEDJOU, CLEMENT G.; PATLOLLA, ANITA K.; SUTTON, DWAYNE J. Heavy Metal Toxicity and the Environment. In Luch, A;Molecular, Clinical and Environmental Toxicology. Experientia Supplementum, v. 101. Springer, Basel, 2012, 564 p.https://doi.org/10.1007/978-3-7643-8340-4_6 /*ref*/TEJADA-TOBAR, CANDELARIA; PAZ-ASTUDILLO, ISABEL-CRISTINA; ACEVEDO-CORREA, DIOFANOR; ESPINOSA-FORTICH, MARÍA; LÓPEZ-BADEL, CRISTINA. Adsorción de Cromo (VI) y Mercurio (II) En Solución Utilizando Jacinto (Eichhornia Crassipes). Biotecnología en el Sector Agropecuario y Agroindustrial, v. 19, n. 1, 2021, p. 54–65. https://doi.org/10.18684/BSAA(19)54-65 /*ref*/THOMAS, JULIYA; ARCHANA, G. Differential Influence of Heavy Metals on Plant Growth Promoting Attributes of Beneficial Microbes and Their Ability to Promote Growth of Vigna Radiata (Mung Bean). Biocatalysis and Agricultural Biotechnology, v. 47, 2023, 102592.https://doi.org/10.1016/j.bcab.2022.102592 /*ref*/UNITED STATES OF AMERICA. AMERICAN SOCIETY FOR TESTING AND MATERIALS (AOAC INTERNATIONAL) Appendix F: Guidelines for Standard Method Performance Requirements. AOAC International and Official Method of Analysis, 2016, p.1–18. /*ref*/WILSCHEFSKI, SCOTT C.; BAXTER, MATTHEW R. Inductively Coupled Plasma Mass Spectrometry: Introduction to Analytical Aspects. Clinical Biochemist Reviews, v. 40, n. 3, 2019, p. 115–133. https://doi.org/10.33176/AACB-19-00024 /*ref*/WILTSCHE, HELMAR; WINKLER, MONIKA; TIRK, PAUL. Matrix Effects of Carbon and Bromine in Inductively Coupled Plasma Optical Emission Spectrometry. Journal of Analytical Atomic Spectrometry, v. 30, n. 10, 2015, p. 2223–2234.http://dx.doi.org/10.1039/C5JA00237K /*ref*/ZAREI, SINA; KARBASSI, ABDOLREZA; SADRINASAB, MASOUD; SARANG, AMIN. Investigating Heavy Metal Pollution in Anzali Coastal Wetland Sediments : A Statistical Approach to Source Identification. Marine Pollution Bulletin, v. 194, Part B, 2023, 115376.https://doi.org/10.1016/j.marpolbul.2023.115376 /*ref*/ Derechos de autor 2023 Universidad del Cauca https://creativecommons.org/licenses/by-nc-nd/4.0 |