Seroprevalence of bovine paratuberculosis with the urea ELISA test in suspicious breeding and milk herds from Buenos Aires province, Argentina

Seroprevalence of bovine paratuberculosis with the urea ELISA test in suspicious breeding and milk herds from Buenos Aires province, Argentina

The results of 17.747 bovine serum samples belonging to 85 beef herds and 5 dairy herds from 14 localities of Buenos Aires province are presented. ELISA is the diagnostic test of choice in cattle with subclinical paratuberculosis, and addition of urea in this assay improves the efficiency to detect antibodies against Mycobacterium avium subsp. paratuberculosis (MAP). Seroprevalence was estimated based on positive and suspicious sera to urea ELISA. Individual seroprevalence for beef and dairy herds was 5.5 % and 13.6 %, respectively, with an overall herd seroprevalence of 78.8 %. In addition, 425 fecal samples were analyzed by microbiological culture. Mycobacterium avium subsp. paratuberculosis was isolated in 67.6 % of positive, 52.7 % of suspicious and 7 % of negative urea ELISA animals. The positive predictive value of urea ELISA was 0.61, and the negative predictive value was 0.93 using Bayesian statistics. These results are an update to the estimated regional paratuberculosis seroprevalence and confirm the usefulness of urea ELISA in the diagnosis and control of paratuberculosis in cattle.

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Main Authors: Alvarado Pinedo, María Fiorella, Di Paolo, Leandro Adrián, Sosa, Pedro Sebastián, Romero, Magali Andrea, Peralta, Luis María, Costa, Enrique Felix, Travería, Gabriel Eduardo
Format: Digital revista
Language:spa
Published: Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata 2019
Online Access:https://revistas.unlp.edu.ar/analecta/article/view/6450
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institution UNLP AR
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country Argentina
countrycode AR
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databasecode rev-analecta-ar
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region America del Sur
libraryname Biblioteca de la Facultad de Ciencias Veterinarias de la UNLP Argentina
language spa
format Digital
author Alvarado Pinedo, María Fiorella
Di Paolo, Leandro Adrián
Sosa, Pedro Sebastián
Romero, Magali Andrea
Peralta, Luis María
Costa, Enrique Felix
Travería, Gabriel Eduardo
spellingShingle Alvarado Pinedo, María Fiorella
Di Paolo, Leandro Adrián
Sosa, Pedro Sebastián
Romero, Magali Andrea
Peralta, Luis María
Costa, Enrique Felix
Travería, Gabriel Eduardo
Seroprevalence of bovine paratuberculosis with the urea ELISA test in suspicious breeding and milk herds from Buenos Aires province, Argentina
author_facet Alvarado Pinedo, María Fiorella
Di Paolo, Leandro Adrián
Sosa, Pedro Sebastián
Romero, Magali Andrea
Peralta, Luis María
Costa, Enrique Felix
Travería, Gabriel Eduardo
author_sort Alvarado Pinedo, María Fiorella
title Seroprevalence of bovine paratuberculosis with the urea ELISA test in suspicious breeding and milk herds from Buenos Aires province, Argentina
title_short Seroprevalence of bovine paratuberculosis with the urea ELISA test in suspicious breeding and milk herds from Buenos Aires province, Argentina
title_full Seroprevalence of bovine paratuberculosis with the urea ELISA test in suspicious breeding and milk herds from Buenos Aires province, Argentina
title_fullStr Seroprevalence of bovine paratuberculosis with the urea ELISA test in suspicious breeding and milk herds from Buenos Aires province, Argentina
title_full_unstemmed Seroprevalence of bovine paratuberculosis with the urea ELISA test in suspicious breeding and milk herds from Buenos Aires province, Argentina
title_sort seroprevalence of bovine paratuberculosis with the urea elisa test in suspicious breeding and milk herds from buenos aires province, argentina
description The results of 17.747 bovine serum samples belonging to 85 beef herds and 5 dairy herds from 14 localities of Buenos Aires province are presented. ELISA is the diagnostic test of choice in cattle with subclinical paratuberculosis, and addition of urea in this assay improves the efficiency to detect antibodies against Mycobacterium avium subsp. paratuberculosis (MAP). Seroprevalence was estimated based on positive and suspicious sera to urea ELISA. Individual seroprevalence for beef and dairy herds was 5.5 % and 13.6 %, respectively, with an overall herd seroprevalence of 78.8 %. In addition, 425 fecal samples were analyzed by microbiological culture. Mycobacterium avium subsp. paratuberculosis was isolated in 67.6 % of positive, 52.7 % of suspicious and 7 % of negative urea ELISA animals. The positive predictive value of urea ELISA was 0.61, and the negative predictive value was 0.93 using Bayesian statistics. These results are an update to the estimated regional paratuberculosis seroprevalence and confirm the usefulness of urea ELISA in the diagnosis and control of paratuberculosis in cattle.
publisher Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata
publishDate 2019
url https://revistas.unlp.edu.ar/analecta/article/view/6450
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spelling rev-analecta-ar-article-64502021-02-03T21:41:01Z Seroprevalence of bovine paratuberculosis with the urea ELISA test in suspicious breeding and milk herds from Buenos Aires province, Argentina Seroprevalencia de paratuberculosis bovina mediante la prueba de ELISA urea en rodeos de cría y de leche con sospecha de la enfermedad, localizados en la provincia de Buenos Aires, Argentina Alvarado Pinedo, María Fiorella Di Paolo, Leandro Adrián Sosa, Pedro Sebastián Romero, Magali Andrea Peralta, Luis María Costa, Enrique Felix Travería, Gabriel Eduardo Ruminants Johne disease diagnosis urea ELISA Rumiantes enfermedad de Johne diagnóstico ELISA urea The results of 17.747 bovine serum samples belonging to 85 beef herds and 5 dairy herds from 14 localities of Buenos Aires province are presented. ELISA is the diagnostic test of choice in cattle with subclinical paratuberculosis, and addition of urea in this assay improves the efficiency to detect antibodies against Mycobacterium avium subsp. paratuberculosis (MAP). Seroprevalence was estimated based on positive and suspicious sera to urea ELISA. Individual seroprevalence for beef and dairy herds was 5.5 % and 13.6 %, respectively, with an overall herd seroprevalence of 78.8 %. In addition, 425 fecal samples were analyzed by microbiological culture. Mycobacterium avium subsp. paratuberculosis was isolated in 67.6 % of positive, 52.7 % of suspicious and 7 % of negative urea ELISA animals. The positive predictive value of urea ELISA was 0.61, and the negative predictive value was 0.93 using Bayesian statistics. These results are an update to the estimated regional paratuberculosis seroprevalence and confirm the usefulness of urea ELISA in the diagnosis and control of paratuberculosis in cattle. Se presentan los resultados obtenidos en 17.747 sueros analizados para el diagnóstico serológico de paratuberculosis bovina, provenientes de 85 rodeos de cría y 5 de leche, ubicados en 14 partidos de la provincia de Buenos Aires. La prueba de ELISA permite diagnosticar esta enfermedad en estadio subclínico, y la incorporación de urea mejora la eficiencia para detectar los anticuerpos específicos contra el Mycobacterium avium subsp. paratuberculosis (MAP). Se estimó la seroprevalencia sobre la base de los resultados obtenidos de animales caracterizados como positivos y sospechosos en la prueba de ELISA urea. Se observó una seroprevalencia individual del 5,5 % en los rodeos de cría y del 13,6 % en los rodeos lecheros, siendo la seroprevalencia predial del 78,8 %. De 425 muestras de heces cultivadas se aisló MAP en el 67,6 % de los animales positivos, del 52,7 % de los sospechosos y del 7 % de los negativos por la prueba de ELISA urea. Mediante estadística bayesiana, el valor predictivo positivo de esta prueba fue de 0,61 y el valor predictivo negativo de 0,93. Nuestros resultados aportan información actualizada sobre la estimación de la seroprevalencia regional de paratuberculosis bovina, la cual es elevada, y confirma la utilidad de la prueba de ELISA urea, desarrollada localmente, en el diagnóstico y control de la enfermedad. Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata 2019-05-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research Articles Trabajos de Investigación application/pdf application/epub+zip application/octet-stream text/html https://revistas.unlp.edu.ar/analecta/article/view/6450 10.24215/15142590e032 Analecta Veterinary; Vol. 39 No. 1 (2019): Analecta Veterinaria; 032 Analecta Veterinaria; Vol. 39 Núm. 1 (2019): Analecta Veterinaria; 032 Analecta Veterinaria; Vol. 39 No. 1 (2019): Analecta Veterinaria; 032 Analecta Veterinaria; v. 39 n. 1 (2019): Analecta Veterinaria; 032 1514-2590 0365-5148 spa https://revistas.unlp.edu.ar/analecta/article/view/6450/6573 https://revistas.unlp.edu.ar/analecta/article/view/6450/6574 https://revistas.unlp.edu.ar/analecta/article/view/6450/6575 https://revistas.unlp.edu.ar/analecta/article/view/6450/8902 Derechos de autor 2019 María Fiorella Alvarado Pinedo, Leandro Adrián Di Paolo, Pedro Sebastián Sosa, Magali Andrea Romero, Luis María Peralta, Enrique Felix Costa, Gabriel Eduardo Travería

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