Molecular Characterization of Group B Streptococcus Serotypes By Multiplex Polymerase Chain Reaction
OBJECTIVE: Group B Streptococcus (GBS) serotypes (Ia, Ib and II to IX) are classified based on variations in their capsular polysaccharide; their prevalence differs between different geographic areas. We examined the prevalence of all GBS serotypes in rectal and vaginal swab samples obtained from 363 pregnant women followed at a Brazilian referral center (Hospital da Mulher Professor Doutor José Aristodemo Pinotti); bacterial susceptibility to antibiotics was further determined. METHOD: Prevalence of positive GBS was evaluated by latex agglutination and by multiplex PCR analysis; bacterial susceptibility to antibiotics, such as clindamycin, erythromycin, levofloxacin, linezolid, penicillin and tetracycline was determined by the disk diffusion method. RESULTS: (a) standard GBS culture and the multiplex PCR analysis tested positive for 83 swabs, collected from 72 women (prevalence of GBS colonization: 72/363; 20%); the most prevalent Serotype was Ia (n=43/83; 52%), followed by serotype V (n=14/83; 17%); according to anatomical origin, serotype Ia accounted for 27/59 (46%) and 16/24 (67%) of the vaginal and rectal samples, respectively; PCR also identified serotypes Ib, II, III and VI. Serotype VI is rarely described and had not been previously reported in Brazil or in Latin America. (b) The latex agglutination test only identified 44 positive samples, all of which were serotyped: 34 of these samples (77%) had serotypes matching those identified by multiplex PCR. (c) Only one sample (serotype Ia) showed resistance to erythromycin and clindamycin. CONCLUSION: Regional studies on GBS serotypes prevalence are essential to guide immunoprophylactic interventions (vaccines) and the implementation of adequate antibiotic prophylaxis or treatment. In this study, the incidence of the serotype VI, a new and rare serotype of GBS was described for the first time in a Brazilian population.
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oai:scielo:S2358-042920170004000062017-09-12Molecular Characterization of Group B Streptococcus Serotypes By Multiplex Polymerase Chain ReactionAndrade,Paula DuranteRusso,Joice de SouzaGouveia,Jéssica BalieroCosta,Cláudia Raquel CantarelliOliveira,Ketti GleyzerGianetti,MichelliAnjos,Emanuel Borges VítorPavan,Tycha Bianca SabainiMartins,Mariana Furquim Da SilvaFranco,JosieleCosta,Maria LauraNomura,Marcelo LuísLevy,Carlos EmílioPassini Júnior,RenatoCosta,Sandra Cecilia Botelho Capsular polysaccharides Group B Streptococcus Molecular serotyping Multiplex PCR Streptococcus agalactiae serotype VI OBJECTIVE: Group B Streptococcus (GBS) serotypes (Ia, Ib and II to IX) are classified based on variations in their capsular polysaccharide; their prevalence differs between different geographic areas. We examined the prevalence of all GBS serotypes in rectal and vaginal swab samples obtained from 363 pregnant women followed at a Brazilian referral center (Hospital da Mulher Professor Doutor José Aristodemo Pinotti); bacterial susceptibility to antibiotics was further determined. METHOD: Prevalence of positive GBS was evaluated by latex agglutination and by multiplex PCR analysis; bacterial susceptibility to antibiotics, such as clindamycin, erythromycin, levofloxacin, linezolid, penicillin and tetracycline was determined by the disk diffusion method. RESULTS: (a) standard GBS culture and the multiplex PCR analysis tested positive for 83 swabs, collected from 72 women (prevalence of GBS colonization: 72/363; 20%); the most prevalent Serotype was Ia (n=43/83; 52%), followed by serotype V (n=14/83; 17%); according to anatomical origin, serotype Ia accounted for 27/59 (46%) and 16/24 (67%) of the vaginal and rectal samples, respectively; PCR also identified serotypes Ib, II, III and VI. Serotype VI is rarely described and had not been previously reported in Brazil or in Latin America. (b) The latex agglutination test only identified 44 positive samples, all of which were serotyped: 34 of these samples (77%) had serotypes matching those identified by multiplex PCR. (c) Only one sample (serotype Ia) showed resistance to erythromycin and clindamycin. CONCLUSION: Regional studies on GBS serotypes prevalence are essential to guide immunoprophylactic interventions (vaccines) and the implementation of adequate antibiotic prophylaxis or treatment. In this study, the incidence of the serotype VI, a new and rare serotype of GBS was described for the first time in a Brazilian population.info:eu-repo/semantics/openAccessMavera Edições Técnicas e Científicas LtdaMedicalExpress v.4 n.4 20172017-08-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2358-04292017000400006en10.5935/medicalexpress.2017.04.06 |
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Andrade,Paula Durante Russo,Joice de Souza Gouveia,Jéssica Baliero Costa,Cláudia Raquel Cantarelli Oliveira,Ketti Gleyzer Gianetti,Michelli Anjos,Emanuel Borges Vítor Pavan,Tycha Bianca Sabaini Martins,Mariana Furquim Da Silva Franco,Josiele Costa,Maria Laura Nomura,Marcelo Luís Levy,Carlos Emílio Passini Júnior,Renato Costa,Sandra Cecilia Botelho |
spellingShingle |
Andrade,Paula Durante Russo,Joice de Souza Gouveia,Jéssica Baliero Costa,Cláudia Raquel Cantarelli Oliveira,Ketti Gleyzer Gianetti,Michelli Anjos,Emanuel Borges Vítor Pavan,Tycha Bianca Sabaini Martins,Mariana Furquim Da Silva Franco,Josiele Costa,Maria Laura Nomura,Marcelo Luís Levy,Carlos Emílio Passini Júnior,Renato Costa,Sandra Cecilia Botelho Molecular Characterization of Group B Streptococcus Serotypes By Multiplex Polymerase Chain Reaction |
author_facet |
Andrade,Paula Durante Russo,Joice de Souza Gouveia,Jéssica Baliero Costa,Cláudia Raquel Cantarelli Oliveira,Ketti Gleyzer Gianetti,Michelli Anjos,Emanuel Borges Vítor Pavan,Tycha Bianca Sabaini Martins,Mariana Furquim Da Silva Franco,Josiele Costa,Maria Laura Nomura,Marcelo Luís Levy,Carlos Emílio Passini Júnior,Renato Costa,Sandra Cecilia Botelho |
author_sort |
Andrade,Paula Durante |
title |
Molecular Characterization of Group B Streptococcus Serotypes By Multiplex Polymerase Chain Reaction |
title_short |
Molecular Characterization of Group B Streptococcus Serotypes By Multiplex Polymerase Chain Reaction |
title_full |
Molecular Characterization of Group B Streptococcus Serotypes By Multiplex Polymerase Chain Reaction |
title_fullStr |
Molecular Characterization of Group B Streptococcus Serotypes By Multiplex Polymerase Chain Reaction |
title_full_unstemmed |
Molecular Characterization of Group B Streptococcus Serotypes By Multiplex Polymerase Chain Reaction |
title_sort |
molecular characterization of group b streptococcus serotypes by multiplex polymerase chain reaction |
description |
OBJECTIVE: Group B Streptococcus (GBS) serotypes (Ia, Ib and II to IX) are classified based on variations in their capsular polysaccharide; their prevalence differs between different geographic areas. We examined the prevalence of all GBS serotypes in rectal and vaginal swab samples obtained from 363 pregnant women followed at a Brazilian referral center (Hospital da Mulher Professor Doutor José Aristodemo Pinotti); bacterial susceptibility to antibiotics was further determined. METHOD: Prevalence of positive GBS was evaluated by latex agglutination and by multiplex PCR analysis; bacterial susceptibility to antibiotics, such as clindamycin, erythromycin, levofloxacin, linezolid, penicillin and tetracycline was determined by the disk diffusion method. RESULTS: (a) standard GBS culture and the multiplex PCR analysis tested positive for 83 swabs, collected from 72 women (prevalence of GBS colonization: 72/363; 20%); the most prevalent Serotype was Ia (n=43/83; 52%), followed by serotype V (n=14/83; 17%); according to anatomical origin, serotype Ia accounted for 27/59 (46%) and 16/24 (67%) of the vaginal and rectal samples, respectively; PCR also identified serotypes Ib, II, III and VI. Serotype VI is rarely described and had not been previously reported in Brazil or in Latin America. (b) The latex agglutination test only identified 44 positive samples, all of which were serotyped: 34 of these samples (77%) had serotypes matching those identified by multiplex PCR. (c) Only one sample (serotype Ia) showed resistance to erythromycin and clindamycin. CONCLUSION: Regional studies on GBS serotypes prevalence are essential to guide immunoprophylactic interventions (vaccines) and the implementation of adequate antibiotic prophylaxis or treatment. In this study, the incidence of the serotype VI, a new and rare serotype of GBS was described for the first time in a Brazilian population. |
publisher |
Mavera Edições Técnicas e Científicas Ltda |
publishDate |
2017 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2358-04292017000400006 |
work_keys_str_mv |
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