Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide

Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide

Abstract Seven imazapyr-tolerant mutant sugarcane plants, previously generated by in vitro mutagenesis, were studied. The imazapyr concentrations required to inhibit their acetolactate synthase (ALS basal activity) (IC50 as μmoles acetoin h-1 mg-1 protein) were 0.77 - 5.36 times greater than that of the N12 ‘parent’. The basal ALS activities of Mut1 and Mut6 were 1.4-fold higher than that of N12. When the mutants were sprayed with Arsenal® GEN 2 (312 and 624 g a.i. imazapyr ha-1), 2 months after field planting, and evaluated 9 months later, live stalk height and number were significantly lowest in Mut2, Mut3 and the control N12. No differences in sucrose, fibre and estimated yield were observed amongst lines in untreated plots. Mutant plants germinated and grew in soil treated with the herbicide (at the lethal dose of 1248 g a.i. ha-1). The Mut lines tested in this study offer improved options for weed control.

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Main Authors: Rutherford,RS, Maphalala,KZ, Koch,AC, Snyman,SJ, Watt,MP
Format: Digital revista
Language:English
Published: Crop Breeding and Applied Biotechnology 2017
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200107
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spelling oai:scielo:S1984-703320170002001072017-03-28Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicideRutherford,RSMaphalala,KZKoch,ACSnyman,SJWatt,MP Acetolactate synthase ethyl methanesulfonate imidazolinone mutation breeding Abstract Seven imazapyr-tolerant mutant sugarcane plants, previously generated by in vitro mutagenesis, were studied. The imazapyr concentrations required to inhibit their acetolactate synthase (ALS basal activity) (IC50 as μmoles acetoin h-1 mg-1 protein) were 0.77 - 5.36 times greater than that of the N12 ‘parent’. The basal ALS activities of Mut1 and Mut6 were 1.4-fold higher than that of N12. When the mutants were sprayed with Arsenal® GEN 2 (312 and 624 g a.i. imazapyr ha-1), 2 months after field planting, and evaluated 9 months later, live stalk height and number were significantly lowest in Mut2, Mut3 and the control N12. No differences in sucrose, fibre and estimated yield were observed amongst lines in untreated plots. Mutant plants germinated and grew in soil treated with the herbicide (at the lethal dose of 1248 g a.i. ha-1). The Mut lines tested in this study offer improved options for weed control.info:eu-repo/semantics/openAccessCrop Breeding and Applied BiotechnologyCrop Breeding and Applied Biotechnology v.17 n.2 20172017-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200107en10.1590/1984-70332017v17n2a17
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Rutherford,RS
Maphalala,KZ
Koch,AC
Snyman,SJ
Watt,MP
spellingShingle Rutherford,RS
Maphalala,KZ
Koch,AC
Snyman,SJ
Watt,MP
Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
author_facet Rutherford,RS
Maphalala,KZ
Koch,AC
Snyman,SJ
Watt,MP
author_sort Rutherford,RS
title Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
title_short Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
title_full Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
title_fullStr Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
title_full_unstemmed Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
title_sort field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
description Abstract Seven imazapyr-tolerant mutant sugarcane plants, previously generated by in vitro mutagenesis, were studied. The imazapyr concentrations required to inhibit their acetolactate synthase (ALS basal activity) (IC50 as μmoles acetoin h-1 mg-1 protein) were 0.77 - 5.36 times greater than that of the N12 ‘parent’. The basal ALS activities of Mut1 and Mut6 were 1.4-fold higher than that of N12. When the mutants were sprayed with Arsenal® GEN 2 (312 and 624 g a.i. imazapyr ha-1), 2 months after field planting, and evaluated 9 months later, live stalk height and number were significantly lowest in Mut2, Mut3 and the control N12. No differences in sucrose, fibre and estimated yield were observed amongst lines in untreated plots. Mutant plants germinated and grew in soil treated with the herbicide (at the lethal dose of 1248 g a.i. ha-1). The Mut lines tested in this study offer improved options for weed control.
publisher Crop Breeding and Applied Biotechnology
publishDate 2017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000200107
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