Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats
Abstract Molecular methods such as Copro-PCR stand out in the diagnosis of T. gondii, because they are highly sensitive and specific, and can distinguish T. gondii from other morphologically similar coccids. The purpose was the detection of Toxoplasma gondii copro-prevalence by polymerase chain reaction in 149 fecal samples from stray and domiciled cats, using three distinct markers (B5-B6, 18S and 529bp RE). Oocysts of T. gondii/H. hammondi were detected in 15.4% by parasitology fecal tests (PFT), and 4% of these oocysts were positively identified as T. gondii by Copro-PCR. The presence of T. gondii genetic material was detected in 16.1%, but 12% of the samples that tested positive by Copro-PCR were negative in PFT. Samples with discordant results were subjected to a new Copro-PCR with 18S marker and a 529, and of the 17 samples, 9 contained T. gondii genetic material. A comparison of the PFT and the molecular methods showed the latter was more sensitive, since it detected 22.1% while the PFT detected 15.4%. Demonstrating the high sensitivity and specificity of the Copro-PCR, particularly with the association of primers (k=0.809), but also confirms the importance of using molecular techniques in laboratories, since Copro-PCR was able to detect samples considered negative by PFT.
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Colégio Brasileiro de Parasitologia Veterinária
2021
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oai:scielo:S1984-296120210002003012021-05-06Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled catsIgreja,Jaqueline Ataíde Silva Lima daRezende,Hanstter Hallison AlvesMelo,Jade de OliveiraGarcia,João LuísMartins,Felippe Danyel CardosoCastro,Ana Maria de Toxoplasma gondii Copro-PCR cats Abstract Molecular methods such as Copro-PCR stand out in the diagnosis of T. gondii, because they are highly sensitive and specific, and can distinguish T. gondii from other morphologically similar coccids. The purpose was the detection of Toxoplasma gondii copro-prevalence by polymerase chain reaction in 149 fecal samples from stray and domiciled cats, using three distinct markers (B5-B6, 18S and 529bp RE). Oocysts of T. gondii/H. hammondi were detected in 15.4% by parasitology fecal tests (PFT), and 4% of these oocysts were positively identified as T. gondii by Copro-PCR. The presence of T. gondii genetic material was detected in 16.1%, but 12% of the samples that tested positive by Copro-PCR were negative in PFT. Samples with discordant results were subjected to a new Copro-PCR with 18S marker and a 529, and of the 17 samples, 9 contained T. gondii genetic material. A comparison of the PFT and the molecular methods showed the latter was more sensitive, since it detected 22.1% while the PFT detected 15.4%. Demonstrating the high sensitivity and specificity of the Copro-PCR, particularly with the association of primers (k=0.809), but also confirms the importance of using molecular techniques in laboratories, since Copro-PCR was able to detect samples considered negative by PFT.info:eu-repo/semantics/openAccessColégio Brasileiro de Parasitologia VeterináriaRevista Brasileira de Parasitologia Veterinária v.30 n.2 20212021-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612021000200301en10.1590/s1984-29612021022 |
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Igreja,Jaqueline Ataíde Silva Lima da Rezende,Hanstter Hallison Alves Melo,Jade de Oliveira Garcia,João Luís Martins,Felippe Danyel Cardoso Castro,Ana Maria de |
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Igreja,Jaqueline Ataíde Silva Lima da Rezende,Hanstter Hallison Alves Melo,Jade de Oliveira Garcia,João Luís Martins,Felippe Danyel Cardoso Castro,Ana Maria de Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats |
author_facet |
Igreja,Jaqueline Ataíde Silva Lima da Rezende,Hanstter Hallison Alves Melo,Jade de Oliveira Garcia,João Luís Martins,Felippe Danyel Cardoso Castro,Ana Maria de |
author_sort |
Igreja,Jaqueline Ataíde Silva Lima da |
title |
Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats |
title_short |
Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats |
title_full |
Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats |
title_fullStr |
Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats |
title_full_unstemmed |
Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats |
title_sort |
copro-pcr in the detection and confirmation of toxoplasma gondii oocysts in feces of stray and domiciled cats |
description |
Abstract Molecular methods such as Copro-PCR stand out in the diagnosis of T. gondii, because they are highly sensitive and specific, and can distinguish T. gondii from other morphologically similar coccids. The purpose was the detection of Toxoplasma gondii copro-prevalence by polymerase chain reaction in 149 fecal samples from stray and domiciled cats, using three distinct markers (B5-B6, 18S and 529bp RE). Oocysts of T. gondii/H. hammondi were detected in 15.4% by parasitology fecal tests (PFT), and 4% of these oocysts were positively identified as T. gondii by Copro-PCR. The presence of T. gondii genetic material was detected in 16.1%, but 12% of the samples that tested positive by Copro-PCR were negative in PFT. Samples with discordant results were subjected to a new Copro-PCR with 18S marker and a 529, and of the 17 samples, 9 contained T. gondii genetic material. A comparison of the PFT and the molecular methods showed the latter was more sensitive, since it detected 22.1% while the PFT detected 15.4%. Demonstrating the high sensitivity and specificity of the Copro-PCR, particularly with the association of primers (k=0.809), but also confirms the importance of using molecular techniques in laboratories, since Copro-PCR was able to detect samples considered negative by PFT. |
publisher |
Colégio Brasileiro de Parasitologia Veterinária |
publishDate |
2021 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612021000200301 |
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