Occurrence of Bartonella genotypes in bats and associated Streblidae flies from Maranhão state, northeastern Brazil
Abstract Bartonella is a genus of emerging zoonotic bacteria that are mainly associated with mammalian erythrocytes and endothelial cells. Bats are natural reservoirs for a variety of important pathogens that impact human and animal health. Recent reports have highlighted the role of bats and bat flies in the maintenance of Bartonella. Here, we showed that none of the 29 bat DNA blood samples obtained from five bat species in São Luís Island, state of Maranhão, northeastern Brazil, were positive for Bartonella in qPCR assays targeting nuoG. On the other hand, three out of 15 DNA samples (20%) from flies in the family Streblidae were positive for Bartonella. The BLASTn results showed that the gltA and rpoB sequences shared identities ranging from 97.2% to 100%, with Bartonella sequences amplified from bats or bat flies from Costa Rica and Brazil. These findings were supported by phylogenetic analyses based on Bayesian inferences. The present study showed that Bartonella genotypes are present in bat flies, thus shedding some light on the distribution of bat fly-related Bartonella genotypes in South America.
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Colégio Brasileiro de Parasitologia Veterinária
2020
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oai:scielo:S1984-296120200004004022020-10-15Occurrence of Bartonella genotypes in bats and associated Streblidae flies from Maranhão state, northeastern BrazilBraga,Maria do Socorro Costa OliveiraGonçalves,Luiz RicardoSilva,Thiago Merighi Vieira daCosta,Francisco BorgesPereira,José GomesSantos,Larissa Sarmento dosCarvalho Neta,Alcina Vieira deArruda,Roberto Carlos Negreiros deMesquita,Eric Takashi Kamakura de CarvalhoChaves,Daniel PrazeresMelo,Ferdinan AlmeidaLopes,Jady LimaMartins,Rayra Tayla BarbosaLima,Mauricio SouzaAmaral,Renan Bressianini doMachado,Rosangela ZacariasAndré,Marcos Rogério Bartonellosis Chiroptera Hippoboscoidea PCR Abstract Bartonella is a genus of emerging zoonotic bacteria that are mainly associated with mammalian erythrocytes and endothelial cells. Bats are natural reservoirs for a variety of important pathogens that impact human and animal health. Recent reports have highlighted the role of bats and bat flies in the maintenance of Bartonella. Here, we showed that none of the 29 bat DNA blood samples obtained from five bat species in São Luís Island, state of Maranhão, northeastern Brazil, were positive for Bartonella in qPCR assays targeting nuoG. On the other hand, three out of 15 DNA samples (20%) from flies in the family Streblidae were positive for Bartonella. The BLASTn results showed that the gltA and rpoB sequences shared identities ranging from 97.2% to 100%, with Bartonella sequences amplified from bats or bat flies from Costa Rica and Brazil. These findings were supported by phylogenetic analyses based on Bayesian inferences. The present study showed that Bartonella genotypes are present in bat flies, thus shedding some light on the distribution of bat fly-related Bartonella genotypes in South America.info:eu-repo/semantics/openAccessColégio Brasileiro de Parasitologia VeterináriaRevista Brasileira de Parasitologia Veterinária v.29 n.4 20202020-01-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612020000400402en10.1590/s1984-29612020088 |
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Braga,Maria do Socorro Costa Oliveira Gonçalves,Luiz Ricardo Silva,Thiago Merighi Vieira da Costa,Francisco Borges Pereira,José Gomes Santos,Larissa Sarmento dos Carvalho Neta,Alcina Vieira de Arruda,Roberto Carlos Negreiros de Mesquita,Eric Takashi Kamakura de Carvalho Chaves,Daniel Prazeres Melo,Ferdinan Almeida Lopes,Jady Lima Martins,Rayra Tayla Barbosa Lima,Mauricio Souza Amaral,Renan Bressianini do Machado,Rosangela Zacarias André,Marcos Rogério |
| spellingShingle |
Braga,Maria do Socorro Costa Oliveira Gonçalves,Luiz Ricardo Silva,Thiago Merighi Vieira da Costa,Francisco Borges Pereira,José Gomes Santos,Larissa Sarmento dos Carvalho Neta,Alcina Vieira de Arruda,Roberto Carlos Negreiros de Mesquita,Eric Takashi Kamakura de Carvalho Chaves,Daniel Prazeres Melo,Ferdinan Almeida Lopes,Jady Lima Martins,Rayra Tayla Barbosa Lima,Mauricio Souza Amaral,Renan Bressianini do Machado,Rosangela Zacarias André,Marcos Rogério Occurrence of Bartonella genotypes in bats and associated Streblidae flies from Maranhão state, northeastern Brazil |
| author_facet |
Braga,Maria do Socorro Costa Oliveira Gonçalves,Luiz Ricardo Silva,Thiago Merighi Vieira da Costa,Francisco Borges Pereira,José Gomes Santos,Larissa Sarmento dos Carvalho Neta,Alcina Vieira de Arruda,Roberto Carlos Negreiros de Mesquita,Eric Takashi Kamakura de Carvalho Chaves,Daniel Prazeres Melo,Ferdinan Almeida Lopes,Jady Lima Martins,Rayra Tayla Barbosa Lima,Mauricio Souza Amaral,Renan Bressianini do Machado,Rosangela Zacarias André,Marcos Rogério |
| author_sort |
Braga,Maria do Socorro Costa Oliveira |
| title |
Occurrence of Bartonella genotypes in bats and associated Streblidae flies from Maranhão state, northeastern Brazil |
| title_short |
Occurrence of Bartonella genotypes in bats and associated Streblidae flies from Maranhão state, northeastern Brazil |
| title_full |
Occurrence of Bartonella genotypes in bats and associated Streblidae flies from Maranhão state, northeastern Brazil |
| title_fullStr |
Occurrence of Bartonella genotypes in bats and associated Streblidae flies from Maranhão state, northeastern Brazil |
| title_full_unstemmed |
Occurrence of Bartonella genotypes in bats and associated Streblidae flies from Maranhão state, northeastern Brazil |
| title_sort |
occurrence of bartonella genotypes in bats and associated streblidae flies from maranhão state, northeastern brazil |
| description |
Abstract Bartonella is a genus of emerging zoonotic bacteria that are mainly associated with mammalian erythrocytes and endothelial cells. Bats are natural reservoirs for a variety of important pathogens that impact human and animal health. Recent reports have highlighted the role of bats and bat flies in the maintenance of Bartonella. Here, we showed that none of the 29 bat DNA blood samples obtained from five bat species in São Luís Island, state of Maranhão, northeastern Brazil, were positive for Bartonella in qPCR assays targeting nuoG. On the other hand, three out of 15 DNA samples (20%) from flies in the family Streblidae were positive for Bartonella. The BLASTn results showed that the gltA and rpoB sequences shared identities ranging from 97.2% to 100%, with Bartonella sequences amplified from bats or bat flies from Costa Rica and Brazil. These findings were supported by phylogenetic analyses based on Bayesian inferences. The present study showed that Bartonella genotypes are present in bat flies, thus shedding some light on the distribution of bat fly-related Bartonella genotypes in South America. |
| publisher |
Colégio Brasileiro de Parasitologia Veterinária |
| publishDate |
2020 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612020000400402 |
| work_keys_str_mv |
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