RESPONSE OF Cattleya forbesii ORCHID TO INCREASING SILICON CONCENTRATIONS IN VITRO
ABSTRACT: Addition of Silicon (Si) to culture media has been shown to improve the development of seedlings grown in vitro, and to reduce losses during the acclimatization phase. The objective of this study was to evaluate the in vitro growth of Cattleya forbesii (Orchidaceae) in MS medium containing five different concentrations of SiO2 (0.0, 0.5, 1.0, 1.5, and 2.0 g·L−1). At day 200, the following variables were measured: number of roots, average length of the root system, leaf area, number of leaves and shoots, shoot height, fresh and dry masses of roots and shoots, water content of roots and shoots, and pH of the culture medium. Most variables decreased as the concentration of Si increased, reducing the in vitro vegetative growth of C. forbesii. Accumulation of Si in leaf tissues was detected by scanning electron microscopy, confirming uptake by plants. The Si source and concentrations tested showed no beneficial effect on in vitro growth of C. forbesii.
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Digital revista |
| Language: | English |
| Published: |
Universidade Federal Rural do Semi-Árido
2016
|
| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-21252016000100018 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
oai:scielo:S1983-21252016000100018 |
|---|---|
| record_format |
ojs |
| spelling |
oai:scielo:S1983-212520160001000182016-04-19RESPONSE OF Cattleya forbesii ORCHID TO INCREASING SILICON CONCENTRATIONS IN VITROCOLOMBO,RONAN CARLOSFAVETTA,VANESSAFARIA,RICARDO TADEU DEANDRADE,FELIPE ARANHA DEMELEM,VANDERLI MARINO Micropropagation Culture medium Amorphous silica. ABSTRACT: Addition of Silicon (Si) to culture media has been shown to improve the development of seedlings grown in vitro, and to reduce losses during the acclimatization phase. The objective of this study was to evaluate the in vitro growth of Cattleya forbesii (Orchidaceae) in MS medium containing five different concentrations of SiO2 (0.0, 0.5, 1.0, 1.5, and 2.0 g·L−1). At day 200, the following variables were measured: number of roots, average length of the root system, leaf area, number of leaves and shoots, shoot height, fresh and dry masses of roots and shoots, water content of roots and shoots, and pH of the culture medium. Most variables decreased as the concentration of Si increased, reducing the in vitro vegetative growth of C. forbesii. Accumulation of Si in leaf tissues was detected by scanning electron microscopy, confirming uptake by plants. The Si source and concentrations tested showed no beneficial effect on in vitro growth of C. forbesii.info:eu-repo/semantics/openAccessUniversidade Federal Rural do Semi-ÁridoRevista Caatinga v.29 n.1 20162016-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-21252016000100018en10.1590/1983-21252016v29n103rc |
| institution |
SCIELO |
| collection |
OJS |
| country |
Brasil |
| countrycode |
BR |
| component |
Revista |
| access |
En linea |
| databasecode |
rev-scielo-br |
| tag |
revista |
| region |
America del Sur |
| libraryname |
SciELO |
| language |
English |
| format |
Digital |
| author |
COLOMBO,RONAN CARLOS FAVETTA,VANESSA FARIA,RICARDO TADEU DE ANDRADE,FELIPE ARANHA DE MELEM,VANDERLI MARINO |
| spellingShingle |
COLOMBO,RONAN CARLOS FAVETTA,VANESSA FARIA,RICARDO TADEU DE ANDRADE,FELIPE ARANHA DE MELEM,VANDERLI MARINO RESPONSE OF Cattleya forbesii ORCHID TO INCREASING SILICON CONCENTRATIONS IN VITRO |
| author_facet |
COLOMBO,RONAN CARLOS FAVETTA,VANESSA FARIA,RICARDO TADEU DE ANDRADE,FELIPE ARANHA DE MELEM,VANDERLI MARINO |
| author_sort |
COLOMBO,RONAN CARLOS |
| title |
RESPONSE OF Cattleya forbesii ORCHID TO INCREASING SILICON CONCENTRATIONS IN VITRO |
| title_short |
RESPONSE OF Cattleya forbesii ORCHID TO INCREASING SILICON CONCENTRATIONS IN VITRO |
| title_full |
RESPONSE OF Cattleya forbesii ORCHID TO INCREASING SILICON CONCENTRATIONS IN VITRO |
| title_fullStr |
RESPONSE OF Cattleya forbesii ORCHID TO INCREASING SILICON CONCENTRATIONS IN VITRO |
| title_full_unstemmed |
RESPONSE OF Cattleya forbesii ORCHID TO INCREASING SILICON CONCENTRATIONS IN VITRO |
| title_sort |
response of cattleya forbesii orchid to increasing silicon concentrations in vitro |
| description |
ABSTRACT: Addition of Silicon (Si) to culture media has been shown to improve the development of seedlings grown in vitro, and to reduce losses during the acclimatization phase. The objective of this study was to evaluate the in vitro growth of Cattleya forbesii (Orchidaceae) in MS medium containing five different concentrations of SiO2 (0.0, 0.5, 1.0, 1.5, and 2.0 g·L−1). At day 200, the following variables were measured: number of roots, average length of the root system, leaf area, number of leaves and shoots, shoot height, fresh and dry masses of roots and shoots, water content of roots and shoots, and pH of the culture medium. Most variables decreased as the concentration of Si increased, reducing the in vitro vegetative growth of C. forbesii. Accumulation of Si in leaf tissues was detected by scanning electron microscopy, confirming uptake by plants. The Si source and concentrations tested showed no beneficial effect on in vitro growth of C. forbesii. |
| publisher |
Universidade Federal Rural do Semi-Árido |
| publishDate |
2016 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-21252016000100018 |
| work_keys_str_mv |
AT colomboronancarlos responseofcattleyaforbesiiorchidtoincreasingsiliconconcentrationsinvitro AT favettavanessa responseofcattleyaforbesiiorchidtoincreasingsiliconconcentrationsinvitro AT fariaricardotadeude responseofcattleyaforbesiiorchidtoincreasingsiliconconcentrationsinvitro AT andradefelipearanhade responseofcattleyaforbesiiorchidtoincreasingsiliconconcentrationsinvitro AT melemvanderlimarino responseofcattleyaforbesiiorchidtoincreasingsiliconconcentrationsinvitro |
| _version_ |
1756436120089919488 |