Morphology and viability of castor bean genotypes pollen grains
ABSTRACT. The objective of this work was to characterize the morphology and viability of the pollen of 15 genotypes of castor bean (Ricinus communis L.) and to generate information that can assist in the selection of highly promising male parents for future use in genetic improvement programs aimed at producing seeds for oil extraction. Acetolysis and scanning electron microscopy was used to characterize the morphology of the pollen. The viability of the pollen grains was estimated by in vitro germination and colorimetric analysis (acetocarmine 2% and 2, 3, 5-triphenyltetrazolium chloride 1%). For the in vitro germination, pollen grains were grown in 10 types of solidified culture medium consisting of different concentrations of sucrose, boric acid, calcium nitrate, magnesium sulfate and potassium nitrate. The pollen grains had the following characteristics: medium size, isopolar and subspheroidal shape, radial symmetry, circular ambit, 3-colporate, elongated endoapertures, tectate exine and granulated sexine. The acetocarmine dye overestimated pollen viability. The media M5 and M8 were the most efficient at promoting the germination of pollen grains. The studied genotypes had high levels of viability and can therefore be used as male parents in genetic improvement programs.
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Editora da Universidade Estadual de Maringá - EDUEM
2016
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oai:scielo:S1807-862120160001000772016-02-25Morphology and viability of castor bean genotypes pollen grainsDiamantino,Maria Selma Alves SilvaCosta,Maria Angélica Pereira de CarvalhoSoares,Taliane LeilaMorais,Daniel VieiraSilva,Simone AlvesSouza,Everton Hilo de Ricinus communis L. in vitro germination colorimetric analysis pollen tube. ABSTRACT. The objective of this work was to characterize the morphology and viability of the pollen of 15 genotypes of castor bean (Ricinus communis L.) and to generate information that can assist in the selection of highly promising male parents for future use in genetic improvement programs aimed at producing seeds for oil extraction. Acetolysis and scanning electron microscopy was used to characterize the morphology of the pollen. The viability of the pollen grains was estimated by in vitro germination and colorimetric analysis (acetocarmine 2% and 2, 3, 5-triphenyltetrazolium chloride 1%). For the in vitro germination, pollen grains were grown in 10 types of solidified culture medium consisting of different concentrations of sucrose, boric acid, calcium nitrate, magnesium sulfate and potassium nitrate. The pollen grains had the following characteristics: medium size, isopolar and subspheroidal shape, radial symmetry, circular ambit, 3-colporate, elongated endoapertures, tectate exine and granulated sexine. The acetocarmine dye overestimated pollen viability. The media M5 and M8 were the most efficient at promoting the germination of pollen grains. The studied genotypes had high levels of viability and can therefore be used as male parents in genetic improvement programs.info:eu-repo/semantics/openAccessEditora da Universidade Estadual de Maringá - EDUEMActa Scientiarum. Agronomy v.38 n.1 20162016-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-86212016000100077en10.4025/actasciagron.v38i1.25981 |
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Diamantino,Maria Selma Alves Silva Costa,Maria Angélica Pereira de Carvalho Soares,Taliane Leila Morais,Daniel Vieira Silva,Simone Alves Souza,Everton Hilo de |
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Diamantino,Maria Selma Alves Silva Costa,Maria Angélica Pereira de Carvalho Soares,Taliane Leila Morais,Daniel Vieira Silva,Simone Alves Souza,Everton Hilo de Morphology and viability of castor bean genotypes pollen grains |
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Diamantino,Maria Selma Alves Silva Costa,Maria Angélica Pereira de Carvalho Soares,Taliane Leila Morais,Daniel Vieira Silva,Simone Alves Souza,Everton Hilo de |
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Diamantino,Maria Selma Alves Silva |
title |
Morphology and viability of castor bean genotypes pollen grains |
title_short |
Morphology and viability of castor bean genotypes pollen grains |
title_full |
Morphology and viability of castor bean genotypes pollen grains |
title_fullStr |
Morphology and viability of castor bean genotypes pollen grains |
title_full_unstemmed |
Morphology and viability of castor bean genotypes pollen grains |
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morphology and viability of castor bean genotypes pollen grains |
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ABSTRACT. The objective of this work was to characterize the morphology and viability of the pollen of 15 genotypes of castor bean (Ricinus communis L.) and to generate information that can assist in the selection of highly promising male parents for future use in genetic improvement programs aimed at producing seeds for oil extraction. Acetolysis and scanning electron microscopy was used to characterize the morphology of the pollen. The viability of the pollen grains was estimated by in vitro germination and colorimetric analysis (acetocarmine 2% and 2, 3, 5-triphenyltetrazolium chloride 1%). For the in vitro germination, pollen grains were grown in 10 types of solidified culture medium consisting of different concentrations of sucrose, boric acid, calcium nitrate, magnesium sulfate and potassium nitrate. The pollen grains had the following characteristics: medium size, isopolar and subspheroidal shape, radial symmetry, circular ambit, 3-colporate, elongated endoapertures, tectate exine and granulated sexine. The acetocarmine dye overestimated pollen viability. The media M5 and M8 were the most efficient at promoting the germination of pollen grains. The studied genotypes had high levels of viability and can therefore be used as male parents in genetic improvement programs. |
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Editora da Universidade Estadual de Maringá - EDUEM |
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2016 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-86212016000100077 |
work_keys_str_mv |
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