Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide

Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide

Abstract: This study evaluated the cytotoxicity and biocompatibility of a new bioceramic endodontic sealer (i.e., Sealer Plus BC) in comparison with those of MTA Fillapex and AH Plus. L929 fibroblasts were cultured and Alamar Blue was used to evaluate cell viability of diluted extracts (1:50, 1:100, and 1:200) from each sealer at 24 h. Polyethylene tubes that were filled with material or empty (as a control) were implanted in the subcutaneous tissue of rats. The rats were killed after 7 and 30 d (n = 8), and the tubes were removed for histological analysis. Parametric data was analyzed using a one-way ANOVA test, and nonparametric data was analyzed via the Kruskal–Wallis test followed by the Dunn test (p < 0.05). A reduction in cell viability was observed in the extracts that were more diluted for Sealer Plus BC when compared to that of Control and AH Plus (p < 0.05). However, the 1:50 dilution of the Sealer Plus BC was similar to that of the Control (p > 0.05). Conversely, more diluted extracts of MTA Fillapex (1:200) and AH Plus (1:100 and 1:200) were similar to the Control (p > 0.05). Histological analysis performed at 7 d did not indicate any significant difference between tissue response for all materials, and the fibrous capsule was thick (p > 0.05). At 30 d, Sealer Plus BC was similar to the Control (p > 0.05) and MTA Fillapex and AH Plus exhibited greater inflammation than the Control (p < 0.05). The fibrous capsule was thin for the Control and for most specimens of Sealer Plus BC and AH Plus. Thus, Sealer Plus BC is biocompatible when compared to MTA Fillapex and AH Plus, and it is less cytotoxic when less-diluted extracts are used.

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Main Authors: Benetti,Francine, de Azevedo Queiroz,Índia Olinta, Oliveira,Pedro Henrique Chaves de, Conti,Leticia Citelli, Azuma,Mariane Maffei, Oliveira,Sandra Helena Penha de, Cintra,Luciano Tavares Angelo
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Pesquisa Odontológica - SBPqO 2019
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100232
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spelling oai:scielo:S1806-832420190001002322019-09-04Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxideBenetti,Francinede Azevedo Queiroz,Índia OlintaOliveira,Pedro Henrique Chaves deConti,Leticia CitelliAzuma,Mariane MaffeiOliveira,Sandra Helena Penha deCintra,Luciano Tavares Angelo Cell Culture Techniques Endodontics Inflammation Materials Testing Abstract: This study evaluated the cytotoxicity and biocompatibility of a new bioceramic endodontic sealer (i.e., Sealer Plus BC) in comparison with those of MTA Fillapex and AH Plus. L929 fibroblasts were cultured and Alamar Blue was used to evaluate cell viability of diluted extracts (1:50, 1:100, and 1:200) from each sealer at 24 h. Polyethylene tubes that were filled with material or empty (as a control) were implanted in the subcutaneous tissue of rats. The rats were killed after 7 and 30 d (n = 8), and the tubes were removed for histological analysis. Parametric data was analyzed using a one-way ANOVA test, and nonparametric data was analyzed via the Kruskal–Wallis test followed by the Dunn test (p < 0.05). A reduction in cell viability was observed in the extracts that were more diluted for Sealer Plus BC when compared to that of Control and AH Plus (p < 0.05). However, the 1:50 dilution of the Sealer Plus BC was similar to that of the Control (p > 0.05). Conversely, more diluted extracts of MTA Fillapex (1:200) and AH Plus (1:100 and 1:200) were similar to the Control (p > 0.05). Histological analysis performed at 7 d did not indicate any significant difference between tissue response for all materials, and the fibrous capsule was thick (p > 0.05). At 30 d, Sealer Plus BC was similar to the Control (p > 0.05) and MTA Fillapex and AH Plus exhibited greater inflammation than the Control (p < 0.05). The fibrous capsule was thin for the Control and for most specimens of Sealer Plus BC and AH Plus. Thus, Sealer Plus BC is biocompatible when compared to MTA Fillapex and AH Plus, and it is less cytotoxic when less-diluted extracts are used.info:eu-repo/semantics/openAccessSociedade Brasileira de Pesquisa Odontológica - SBPqOBrazilian Oral Research v.33 20192019-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100232en10.1590/1807-3107bor-2019.vol33.0042
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country Brasil
countrycode BR
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access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Benetti,Francine
de Azevedo Queiroz,Índia Olinta
Oliveira,Pedro Henrique Chaves de
Conti,Leticia Citelli
Azuma,Mariane Maffei
Oliveira,Sandra Helena Penha de
Cintra,Luciano Tavares Angelo
spellingShingle Benetti,Francine
de Azevedo Queiroz,Índia Olinta
Oliveira,Pedro Henrique Chaves de
Conti,Leticia Citelli
Azuma,Mariane Maffei
Oliveira,Sandra Helena Penha de
Cintra,Luciano Tavares Angelo
Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide
author_facet Benetti,Francine
de Azevedo Queiroz,Índia Olinta
Oliveira,Pedro Henrique Chaves de
Conti,Leticia Citelli
Azuma,Mariane Maffei
Oliveira,Sandra Helena Penha de
Cintra,Luciano Tavares Angelo
author_sort Benetti,Francine
title Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide
title_short Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide
title_full Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide
title_fullStr Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide
title_full_unstemmed Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide
title_sort cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide
description Abstract: This study evaluated the cytotoxicity and biocompatibility of a new bioceramic endodontic sealer (i.e., Sealer Plus BC) in comparison with those of MTA Fillapex and AH Plus. L929 fibroblasts were cultured and Alamar Blue was used to evaluate cell viability of diluted extracts (1:50, 1:100, and 1:200) from each sealer at 24 h. Polyethylene tubes that were filled with material or empty (as a control) were implanted in the subcutaneous tissue of rats. The rats were killed after 7 and 30 d (n = 8), and the tubes were removed for histological analysis. Parametric data was analyzed using a one-way ANOVA test, and nonparametric data was analyzed via the Kruskal–Wallis test followed by the Dunn test (p < 0.05). A reduction in cell viability was observed in the extracts that were more diluted for Sealer Plus BC when compared to that of Control and AH Plus (p < 0.05). However, the 1:50 dilution of the Sealer Plus BC was similar to that of the Control (p > 0.05). Conversely, more diluted extracts of MTA Fillapex (1:200) and AH Plus (1:100 and 1:200) were similar to the Control (p > 0.05). Histological analysis performed at 7 d did not indicate any significant difference between tissue response for all materials, and the fibrous capsule was thick (p > 0.05). At 30 d, Sealer Plus BC was similar to the Control (p > 0.05) and MTA Fillapex and AH Plus exhibited greater inflammation than the Control (p < 0.05). The fibrous capsule was thin for the Control and for most specimens of Sealer Plus BC and AH Plus. Thus, Sealer Plus BC is biocompatible when compared to MTA Fillapex and AH Plus, and it is less cytotoxic when less-diluted extracts are used.
publisher Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publishDate 2019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100232
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