Role of adipose tissue-derived stem cells in the progression of renal disease
ABSTRACT Objective: To analyze the role of adipose tissue-derived stem cells in reducing the progression of renal fibrosis. Methods: adipose tissue-derived stem cells were isolated from C57Bl/6 mice and characterized by cytometry and differentiation. Renal fibrosis was established after unilateral clamping of the renal pedicle for 1 hour. Four hours after reperfusion, 2.105 adipose tissue-derived stem cells were administered intraperitoneally and the animals were followed for 24 hours during 6 weeks. In another experimental group, 2.105 adipose tissue-derived stem cells were administered only after 6 weeks of reperfusion, and they were euthanized and studied 4 weeks later. Twenty-four hours after reperfusion, the animals treated with adipose tissue-derived stem cells displayed reduced renal and tubular dysfunction and an increase of the regenerative process. Renal expression of IL-6 and TNF mRNA were decreased in the animals treated with adipose tissue-derived stem cells, while the levels of IL-4, IL-10, and HO-1 were increased, despite the fact that adipose tissue-derived stem cells were not observed in the kidneys via SRY analysis. Results: In 6 weeks, the kidneys of non-treated animals decreased in size, and the kidneys of the animals treated with adipose tissue-derived stem cells remained at normal size and display less deposition of type 1 collagen and FSP-1. The renal protection observed in animals treated with adipose tissue-derived stem cells was followed by a drop in serum levels of TNF-α, KC, RANTES, and IL-1a. Treatment with adipose tissue-derived stem cells after 6 weeks, when the animals already displayed established fibrosis, demonstrated an improvement in functional parameters and less fibrosis analyzed by Picrosirius stain, as well as a reduction of the expression of type 1 collagen and vimentin mRNA. Conclusion: Treatment with adipose tissue-derived stem cells may deter the progression of renal fibrosis by modulation of the early inflammatory response, likely via reduction of the epithelial-mesenchymal transition.
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Instituto Israelita de Ensino e Pesquisa Albert Einstein
2011
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oai:scielo:S1679-450820110001000362017-03-14Role of adipose tissue-derived stem cells in the progression of renal diseaseDonizetti-Oliveira,CassianoSemedo,PatriciaBurgos-Silva,MarinaCenedeze,Marco AntonioMalheiros,Denise Maria Avancini CostaReis,Marlene Antônia dosPacheco-Silva,AlvaroCâmara,Niels Olsen Saraiva Mesenchymal stem cells Renal insufficiency, acute Reperfusion injury Fibrosis Inflammation ABSTRACT Objective: To analyze the role of adipose tissue-derived stem cells in reducing the progression of renal fibrosis. Methods: adipose tissue-derived stem cells were isolated from C57Bl/6 mice and characterized by cytometry and differentiation. Renal fibrosis was established after unilateral clamping of the renal pedicle for 1 hour. Four hours after reperfusion, 2.105 adipose tissue-derived stem cells were administered intraperitoneally and the animals were followed for 24 hours during 6 weeks. In another experimental group, 2.105 adipose tissue-derived stem cells were administered only after 6 weeks of reperfusion, and they were euthanized and studied 4 weeks later. Twenty-four hours after reperfusion, the animals treated with adipose tissue-derived stem cells displayed reduced renal and tubular dysfunction and an increase of the regenerative process. Renal expression of IL-6 and TNF mRNA were decreased in the animals treated with adipose tissue-derived stem cells, while the levels of IL-4, IL-10, and HO-1 were increased, despite the fact that adipose tissue-derived stem cells were not observed in the kidneys via SRY analysis. Results: In 6 weeks, the kidneys of non-treated animals decreased in size, and the kidneys of the animals treated with adipose tissue-derived stem cells remained at normal size and display less deposition of type 1 collagen and FSP-1. The renal protection observed in animals treated with adipose tissue-derived stem cells was followed by a drop in serum levels of TNF-α, KC, RANTES, and IL-1a. Treatment with adipose tissue-derived stem cells after 6 weeks, when the animals already displayed established fibrosis, demonstrated an improvement in functional parameters and less fibrosis analyzed by Picrosirius stain, as well as a reduction of the expression of type 1 collagen and vimentin mRNA. Conclusion: Treatment with adipose tissue-derived stem cells may deter the progression of renal fibrosis by modulation of the early inflammatory response, likely via reduction of the epithelial-mesenchymal transition.info:eu-repo/semantics/openAccessInstituto Israelita de Ensino e Pesquisa Albert Einsteineinstein (São Paulo) v.9 n.1 20112011-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082011000100036en10.1590/s1679-45082011ao1833 |
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Donizetti-Oliveira,Cassiano Semedo,Patricia Burgos-Silva,Marina Cenedeze,Marco Antonio Malheiros,Denise Maria Avancini Costa Reis,Marlene Antônia dos Pacheco-Silva,Alvaro Câmara,Niels Olsen Saraiva |
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Donizetti-Oliveira,Cassiano Semedo,Patricia Burgos-Silva,Marina Cenedeze,Marco Antonio Malheiros,Denise Maria Avancini Costa Reis,Marlene Antônia dos Pacheco-Silva,Alvaro Câmara,Niels Olsen Saraiva Role of adipose tissue-derived stem cells in the progression of renal disease |
author_facet |
Donizetti-Oliveira,Cassiano Semedo,Patricia Burgos-Silva,Marina Cenedeze,Marco Antonio Malheiros,Denise Maria Avancini Costa Reis,Marlene Antônia dos Pacheco-Silva,Alvaro Câmara,Niels Olsen Saraiva |
author_sort |
Donizetti-Oliveira,Cassiano |
title |
Role of adipose tissue-derived stem cells in the progression of renal disease |
title_short |
Role of adipose tissue-derived stem cells in the progression of renal disease |
title_full |
Role of adipose tissue-derived stem cells in the progression of renal disease |
title_fullStr |
Role of adipose tissue-derived stem cells in the progression of renal disease |
title_full_unstemmed |
Role of adipose tissue-derived stem cells in the progression of renal disease |
title_sort |
role of adipose tissue-derived stem cells in the progression of renal disease |
description |
ABSTRACT Objective: To analyze the role of adipose tissue-derived stem cells in reducing the progression of renal fibrosis. Methods: adipose tissue-derived stem cells were isolated from C57Bl/6 mice and characterized by cytometry and differentiation. Renal fibrosis was established after unilateral clamping of the renal pedicle for 1 hour. Four hours after reperfusion, 2.105 adipose tissue-derived stem cells were administered intraperitoneally and the animals were followed for 24 hours during 6 weeks. In another experimental group, 2.105 adipose tissue-derived stem cells were administered only after 6 weeks of reperfusion, and they were euthanized and studied 4 weeks later. Twenty-four hours after reperfusion, the animals treated with adipose tissue-derived stem cells displayed reduced renal and tubular dysfunction and an increase of the regenerative process. Renal expression of IL-6 and TNF mRNA were decreased in the animals treated with adipose tissue-derived stem cells, while the levels of IL-4, IL-10, and HO-1 were increased, despite the fact that adipose tissue-derived stem cells were not observed in the kidneys via SRY analysis. Results: In 6 weeks, the kidneys of non-treated animals decreased in size, and the kidneys of the animals treated with adipose tissue-derived stem cells remained at normal size and display less deposition of type 1 collagen and FSP-1. The renal protection observed in animals treated with adipose tissue-derived stem cells was followed by a drop in serum levels of TNF-α, KC, RANTES, and IL-1a. Treatment with adipose tissue-derived stem cells after 6 weeks, when the animals already displayed established fibrosis, demonstrated an improvement in functional parameters and less fibrosis analyzed by Picrosirius stain, as well as a reduction of the expression of type 1 collagen and vimentin mRNA. Conclusion: Treatment with adipose tissue-derived stem cells may deter the progression of renal fibrosis by modulation of the early inflammatory response, likely via reduction of the epithelial-mesenchymal transition. |
publisher |
Instituto Israelita de Ensino e Pesquisa Albert Einstein |
publishDate |
2011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082011000100036 |
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