Effects of mineral trioxide aggregate, BiodentineTM and calcium hydroxide on viability, proliferation, migration and differentiation of stem cells from human exfoliated deciduous teeth
Abstract Objective: The aim of the study was to evaluate the effects of the capping materials mineral trioxide aggregate (MTA), calcium hydroxide (CH) and BiodentineTM (BD) on stem cells from human exfoliated deciduous teeth (SHED) in vitro. Material and Methods: SHED were cultured for 1 – 7 days in medium conditioned by incubation with MTA, BD or CH (1 mg/mL), and tested for viability (MTT assay) and proliferation (SRB assay). Also, the migration of serum-starved SHED towards conditioned media was assayed in companion plates, with 8 μm-pore-sized membranes, for 24 h. Gene expression of dentin matrix protein-1 (DMP-1) was evaluated by reverse-transcription polymerase chain reaction. Regular culture medium with 10% FBS (without conditioning) and culture medium supplemented with 20% FBS were used as controls. Results: MTA, CH and BD conditioned media maintained cell viability and allowed continuous SHED proliferation, with CH conditioned medium causing the highest positive effect on proliferation at the end of the treatment period (compared with BD and MTA) (p<0.05). In contrast, we observed increased SHED migration towards BD and MTA conditioned media (compared with CH) (p<0.05). A greater amount of DMP-1 gene was expressed in MTA group compared with the other groups from day 7 up to day 21. Conclusion: Our results show that the three capping materials are biocompatible, maintain viability and stimulate proliferation, migration and differentiation in a key dental stem cell population.
Main Authors: | Araújo,Leandro Borges, Cosme-Silva,Leopoldo, Fernandes,Ana Paula, Oliveira,Thais Marchini de, Cavalcanti,Bruno das Neves, Gomes Filho,João Eduardo, Sakai,Vivien Thiemy |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Faculdade De Odontologia De Bauru - USP
2018
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100417 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
BiodentineTM is cytocompatible with human primary osteoblasts
by: SCELZA,Miriam Zaccaro, et al.
Published: (2017) -
Enhanced bioactive properties of BiodentineTM modified with bioactive glass nanoparticles
by: CORRAL NUÑEZ,Camila, et al.
Published: (2017) -
Effects of low-level laser therapy on stem cells from human exfoliated deciduous teeth
by: FERNANDES,Ana Paula, et al.
Published: (2016) -
Comparison between calcium hydroxide mixtures and mineral trioxide aggregate in primary teeth pulpotomy: a randomized controlled trial
by: Silva,Lidiane Lucas Costa e, et al.
Published: (2019) -
Surface and vertical dimensional changes of mineral trioxide aggregate and biodentine in different environmental conditions
by: Aksel,Hacer, et al.
Published: (2019)