Molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from Brazil
Abstract Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats. The recovered ureaplasma were from sheep and goats from animals without any reproductive disorder symptoms, but not all animals presented positive cultures. In sheep, 17 (68%) cultures of vulvovaginal mucous were positive for ureaplasma and 11 (27%) samples of semen presented positive cultures in animals with clinical signs of orchitis, balanoposthitis or low sperm motility. In goats four ureaplasma isolates were obtained from vulvovaginal mucus, but the semen samples were all negative. The isolates were submitted to Pulsed-field gel electrophoresis methodology and their 16S rRNA genes were sequenced. Fifty percent of ureaplasma recovered from sheep allowed for PFGE typing. Eleven isolates showed eight profiles genetically close to the bovine ureaplasmas. The 16S rRNA gene sequencing showed differences or similarities of isolates from sheep and goats, and the reference strains of bovine and human ureaplasma. Four clinical isolates from sheep were grouped separately. The studied ureaplasma isolates showed to be a diverse group of mollicutes.
Main Authors: | , , , , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Microbiologia
2017
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300560 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S1517-83822017000300560 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S1517-838220170003005602017-07-31Molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from BrazilOliveira,Rosângela C.Yamaguti,MaurícioMarques,Lucas M.Buzinhani,MelissaRizzo,HuberMeira-Junior,Enoch Brandão de SouzaGuimarães,Ana Márcia S.Gregory,LilianTimenetsky,Jorge Ureaplasma Ovine Caprine Molecular characterization Abstract Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats. The recovered ureaplasma were from sheep and goats from animals without any reproductive disorder symptoms, but not all animals presented positive cultures. In sheep, 17 (68%) cultures of vulvovaginal mucous were positive for ureaplasma and 11 (27%) samples of semen presented positive cultures in animals with clinical signs of orchitis, balanoposthitis or low sperm motility. In goats four ureaplasma isolates were obtained from vulvovaginal mucus, but the semen samples were all negative. The isolates were submitted to Pulsed-field gel electrophoresis methodology and their 16S rRNA genes were sequenced. Fifty percent of ureaplasma recovered from sheep allowed for PFGE typing. Eleven isolates showed eight profiles genetically close to the bovine ureaplasmas. The 16S rRNA gene sequencing showed differences or similarities of isolates from sheep and goats, and the reference strains of bovine and human ureaplasma. Four clinical isolates from sheep were grouped separately. The studied ureaplasma isolates showed to be a diverse group of mollicutes.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology v.48 n.3 20172017-07-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300560en10.1016/j.bjm.2016.09.020 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Oliveira,Rosângela C. Yamaguti,Maurício Marques,Lucas M. Buzinhani,Melissa Rizzo,Huber Meira-Junior,Enoch Brandão de Souza Guimarães,Ana Márcia S. Gregory,Lilian Timenetsky,Jorge |
spellingShingle |
Oliveira,Rosângela C. Yamaguti,Maurício Marques,Lucas M. Buzinhani,Melissa Rizzo,Huber Meira-Junior,Enoch Brandão de Souza Guimarães,Ana Márcia S. Gregory,Lilian Timenetsky,Jorge Molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from Brazil |
author_facet |
Oliveira,Rosângela C. Yamaguti,Maurício Marques,Lucas M. Buzinhani,Melissa Rizzo,Huber Meira-Junior,Enoch Brandão de Souza Guimarães,Ana Márcia S. Gregory,Lilian Timenetsky,Jorge |
author_sort |
Oliveira,Rosângela C. |
title |
Molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from Brazil |
title_short |
Molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from Brazil |
title_full |
Molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from Brazil |
title_fullStr |
Molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from Brazil |
title_full_unstemmed |
Molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from Brazil |
title_sort |
molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from brazil |
description |
Abstract Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats. The recovered ureaplasma were from sheep and goats from animals without any reproductive disorder symptoms, but not all animals presented positive cultures. In sheep, 17 (68%) cultures of vulvovaginal mucous were positive for ureaplasma and 11 (27%) samples of semen presented positive cultures in animals with clinical signs of orchitis, balanoposthitis or low sperm motility. In goats four ureaplasma isolates were obtained from vulvovaginal mucus, but the semen samples were all negative. The isolates were submitted to Pulsed-field gel electrophoresis methodology and their 16S rRNA genes were sequenced. Fifty percent of ureaplasma recovered from sheep allowed for PFGE typing. Eleven isolates showed eight profiles genetically close to the bovine ureaplasmas. The 16S rRNA gene sequencing showed differences or similarities of isolates from sheep and goats, and the reference strains of bovine and human ureaplasma. Four clinical isolates from sheep were grouped separately. The studied ureaplasma isolates showed to be a diverse group of mollicutes. |
publisher |
Sociedade Brasileira de Microbiologia |
publishDate |
2017 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300560 |
work_keys_str_mv |
AT oliveirarosangelac molecularcharacterizationofureaplasmasisolatedfromreproductivetractofgoatsandsheepfrombrazil AT yamagutimauricio molecularcharacterizationofureaplasmasisolatedfromreproductivetractofgoatsandsheepfrombrazil AT marqueslucasm molecularcharacterizationofureaplasmasisolatedfromreproductivetractofgoatsandsheepfrombrazil AT buzinhanimelissa molecularcharacterizationofureaplasmasisolatedfromreproductivetractofgoatsandsheepfrombrazil AT rizzohuber molecularcharacterizationofureaplasmasisolatedfromreproductivetractofgoatsandsheepfrombrazil AT meirajuniorenochbrandaodesouza molecularcharacterizationofureaplasmasisolatedfromreproductivetractofgoatsandsheepfrombrazil AT guimaraesanamarcias molecularcharacterizationofureaplasmasisolatedfromreproductivetractofgoatsandsheepfrombrazil AT gregorylilian molecularcharacterizationofureaplasmasisolatedfromreproductivetractofgoatsandsheepfrombrazil AT timenetskyjorge molecularcharacterizationofureaplasmasisolatedfromreproductivetractofgoatsandsheepfrombrazil |
_version_ |
1756425123979591680 |