Comparison of culture and PCR methods in the diagnosis of bacterial meningitis

Comparison of culture and PCR methods in the diagnosis of bacterial meningitis

Abstract Our aim in this study is to compare the standard culture method with the multiplex PCR and the Speed-Oligo® Bacterial Meningitis Test (SO-BMT) – a hybridization-based molecular test method – during the CSF examination of the patients with the pre-diagnosis of acute bacterial meningitis. For the purposes of this study, patients with acute bacterial meningitis treated at the Dicle University Medical Faculty Hospital, Infectious Diseases and Clinical Microbiology Clinic between December 2009 and April 2012 were retrospectively evaluated. The diagnosis of bacterial meningitis was made based on the clinical findings, laboratory test anomalies, CSF analysis results, and the radiological images. Growth was observed in the CSF cultures of 10 out of the 57 patients included in the study (17.5%) and Streptococcus pneumoniae was isolated in all of them. The CSF samples of 34 patients (59.6%) were positive according to the SO-BMT and S. pneumoniae was detected in 33 of the samples (97.05%), while Neisseria meningitidis was found in 1 sample (2.95%). In a total of 10 patients, S. pneumoniae was both isolated in the CSF culture and detected in the SO-BMT. The culture and the SO-BMT were negative in 23 of the CSF samples. There was no sample in which the CSF culture was positive although the SO-BMT was negative. While SO-BMT seems to be a more efficient method than bacterial culturing to determine the pathogens that most commonly cause bacterial meningitis in adults, further studies conducted on larger populations are needed in order to assess its efficiency and uses.

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Main Authors: Başpınar,Emel Ödemiş, Dayan,Saim, Bekçibaşı,Muhammed, Tekin,Recep, Ayaz,Celal, Deveci,Özcan, Hoşoğlu,Salih
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Microbiologia 2017
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200232
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spelling oai:scielo:S1517-838220170002002322017-05-11Comparison of culture and PCR methods in the diagnosis of bacterial meningitisBaşpınar,Emel ÖdemişDayan,SaimBekçibaşı,MuhammedTekin,RecepAyaz,CelalDeveci,ÖzcanHoşoğlu,Salih Bacterial meningitis Culture Speed-Oligo PCR Abstract Our aim in this study is to compare the standard culture method with the multiplex PCR and the Speed-Oligo® Bacterial Meningitis Test (SO-BMT) – a hybridization-based molecular test method – during the CSF examination of the patients with the pre-diagnosis of acute bacterial meningitis. For the purposes of this study, patients with acute bacterial meningitis treated at the Dicle University Medical Faculty Hospital, Infectious Diseases and Clinical Microbiology Clinic between December 2009 and April 2012 were retrospectively evaluated. The diagnosis of bacterial meningitis was made based on the clinical findings, laboratory test anomalies, CSF analysis results, and the radiological images. Growth was observed in the CSF cultures of 10 out of the 57 patients included in the study (17.5%) and Streptococcus pneumoniae was isolated in all of them. The CSF samples of 34 patients (59.6%) were positive according to the SO-BMT and S. pneumoniae was detected in 33 of the samples (97.05%), while Neisseria meningitidis was found in 1 sample (2.95%). In a total of 10 patients, S. pneumoniae was both isolated in the CSF culture and detected in the SO-BMT. The culture and the SO-BMT were negative in 23 of the CSF samples. There was no sample in which the CSF culture was positive although the SO-BMT was negative. While SO-BMT seems to be a more efficient method than bacterial culturing to determine the pathogens that most commonly cause bacterial meningitis in adults, further studies conducted on larger populations are needed in order to assess its efficiency and uses.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology v.48 n.2 20172017-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200232en10.1016/j.bjm.2016.06.014
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libraryname SciELO
language English
format Digital
author Başpınar,Emel Ödemiş
Dayan,Saim
Bekçibaşı,Muhammed
Tekin,Recep
Ayaz,Celal
Deveci,Özcan
Hoşoğlu,Salih
spellingShingle Başpınar,Emel Ödemiş
Dayan,Saim
Bekçibaşı,Muhammed
Tekin,Recep
Ayaz,Celal
Deveci,Özcan
Hoşoğlu,Salih
Comparison of culture and PCR methods in the diagnosis of bacterial meningitis
author_facet Başpınar,Emel Ödemiş
Dayan,Saim
Bekçibaşı,Muhammed
Tekin,Recep
Ayaz,Celal
Deveci,Özcan
Hoşoğlu,Salih
author_sort Başpınar,Emel Ödemiş
title Comparison of culture and PCR methods in the diagnosis of bacterial meningitis
title_short Comparison of culture and PCR methods in the diagnosis of bacterial meningitis
title_full Comparison of culture and PCR methods in the diagnosis of bacterial meningitis
title_fullStr Comparison of culture and PCR methods in the diagnosis of bacterial meningitis
title_full_unstemmed Comparison of culture and PCR methods in the diagnosis of bacterial meningitis
title_sort comparison of culture and pcr methods in the diagnosis of bacterial meningitis
description Abstract Our aim in this study is to compare the standard culture method with the multiplex PCR and the Speed-Oligo® Bacterial Meningitis Test (SO-BMT) – a hybridization-based molecular test method – during the CSF examination of the patients with the pre-diagnosis of acute bacterial meningitis. For the purposes of this study, patients with acute bacterial meningitis treated at the Dicle University Medical Faculty Hospital, Infectious Diseases and Clinical Microbiology Clinic between December 2009 and April 2012 were retrospectively evaluated. The diagnosis of bacterial meningitis was made based on the clinical findings, laboratory test anomalies, CSF analysis results, and the radiological images. Growth was observed in the CSF cultures of 10 out of the 57 patients included in the study (17.5%) and Streptococcus pneumoniae was isolated in all of them. The CSF samples of 34 patients (59.6%) were positive according to the SO-BMT and S. pneumoniae was detected in 33 of the samples (97.05%), while Neisseria meningitidis was found in 1 sample (2.95%). In a total of 10 patients, S. pneumoniae was both isolated in the CSF culture and detected in the SO-BMT. The culture and the SO-BMT were negative in 23 of the CSF samples. There was no sample in which the CSF culture was positive although the SO-BMT was negative. While SO-BMT seems to be a more efficient method than bacterial culturing to determine the pathogens that most commonly cause bacterial meningitis in adults, further studies conducted on larger populations are needed in order to assess its efficiency and uses.
publisher Sociedade Brasileira de Microbiologia
publishDate 2017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200232
work_keys_str_mv AT baspınaremelodemis comparisonofcultureandpcrmethodsinthediagnosisofbacterialmeningitis
AT dayansaim comparisonofcultureandpcrmethodsinthediagnosisofbacterialmeningitis
AT bekcibasımuhammed comparisonofcultureandpcrmethodsinthediagnosisofbacterialmeningitis
AT tekinrecep comparisonofcultureandpcrmethodsinthediagnosisofbacterialmeningitis
AT ayazcelal comparisonofcultureandpcrmethodsinthediagnosisofbacterialmeningitis
AT deveciozcan comparisonofcultureandpcrmethodsinthediagnosisofbacterialmeningitis
AT hosoglusalih comparisonofcultureandpcrmethodsinthediagnosisofbacterialmeningitis
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