Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
ABSTRACT The ability of pathogens to survive cheese ripening is a food-security concern. Therefore, this study aimed to evaluate the performance of two alternative methods of analysis of Listeria during the ripening of artisanal Minas cheese. These methods were tested and compared with the conventional method: Lateral Flow System™, in cheeses produced on laboratory scale using raw milk collected from different farms and inoculated with Listeria innocua; and VIDAS®-LMO, in cheese samples collected from different manufacturers in Serro, Minas Gerais, Brazil. These samples were also characterized in terms of lactic acid bacteria, coliforms and physical-chemical analysis. In the inoculated samples, L. innocua was detected by Lateral Flow System™ method with 33% false-negative and 68% accuracy results. L. innocua was only detected in the inoculated samples by the conventional method at 60-days of cheese ripening. L. monocytogenes was not detected by the conventional and the VIDAS®-LMO methods in cheese samples collected from different manufacturers, which impairs evaluating the performance of this alternative method. We concluded that the conventional method provided a better recovery of L. innocua throughout cheese ripening, being able to detect L. innocua at 60-day, aging period which is required by the current legislation.
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Sociedade Brasileira de Microbiologia
2016
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oai:scielo:S1517-838220160003007492016-08-02Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripeningMata,Gardênia Márcia Silva CamposMartins,EvandroMachado,Solimar GonçalvesPinto,Maximiliano SoaresCarvalho,Antônio Fernandes deVanetti,Maria Cristina Dantas Listeria Raw milk cheese Alternative methods ABSTRACT The ability of pathogens to survive cheese ripening is a food-security concern. Therefore, this study aimed to evaluate the performance of two alternative methods of analysis of Listeria during the ripening of artisanal Minas cheese. These methods were tested and compared with the conventional method: Lateral Flow System™, in cheeses produced on laboratory scale using raw milk collected from different farms and inoculated with Listeria innocua; and VIDAS®-LMO, in cheese samples collected from different manufacturers in Serro, Minas Gerais, Brazil. These samples were also characterized in terms of lactic acid bacteria, coliforms and physical-chemical analysis. In the inoculated samples, L. innocua was detected by Lateral Flow System™ method with 33% false-negative and 68% accuracy results. L. innocua was only detected in the inoculated samples by the conventional method at 60-days of cheese ripening. L. monocytogenes was not detected by the conventional and the VIDAS®-LMO methods in cheese samples collected from different manufacturers, which impairs evaluating the performance of this alternative method. We concluded that the conventional method provided a better recovery of L. innocua throughout cheese ripening, being able to detect L. innocua at 60-day, aging period which is required by the current legislation.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology v.47 n.3 20162016-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300749en10.1016/j.bjm.2016.04.006 |
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Mata,Gardênia Márcia Silva Campos Martins,Evandro Machado,Solimar Gonçalves Pinto,Maximiliano Soares Carvalho,Antônio Fernandes de Vanetti,Maria Cristina Dantas |
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Mata,Gardênia Márcia Silva Campos Martins,Evandro Machado,Solimar Gonçalves Pinto,Maximiliano Soares Carvalho,Antônio Fernandes de Vanetti,Maria Cristina Dantas Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening |
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Mata,Gardênia Márcia Silva Campos Martins,Evandro Machado,Solimar Gonçalves Pinto,Maximiliano Soares Carvalho,Antônio Fernandes de Vanetti,Maria Cristina Dantas |
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Mata,Gardênia Márcia Silva Campos |
title |
Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening |
title_short |
Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening |
title_full |
Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening |
title_fullStr |
Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening |
title_full_unstemmed |
Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening |
title_sort |
performance of two alternative methods for listeria detection throughout serro minas cheese ripening |
description |
ABSTRACT The ability of pathogens to survive cheese ripening is a food-security concern. Therefore, this study aimed to evaluate the performance of two alternative methods of analysis of Listeria during the ripening of artisanal Minas cheese. These methods were tested and compared with the conventional method: Lateral Flow System™, in cheeses produced on laboratory scale using raw milk collected from different farms and inoculated with Listeria innocua; and VIDAS®-LMO, in cheese samples collected from different manufacturers in Serro, Minas Gerais, Brazil. These samples were also characterized in terms of lactic acid bacteria, coliforms and physical-chemical analysis. In the inoculated samples, L. innocua was detected by Lateral Flow System™ method with 33% false-negative and 68% accuracy results. L. innocua was only detected in the inoculated samples by the conventional method at 60-days of cheese ripening. L. monocytogenes was not detected by the conventional and the VIDAS®-LMO methods in cheese samples collected from different manufacturers, which impairs evaluating the performance of this alternative method. We concluded that the conventional method provided a better recovery of L. innocua throughout cheese ripening, being able to detect L. innocua at 60-day, aging period which is required by the current legislation. |
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Sociedade Brasileira de Microbiologia |
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2016 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300749 |
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