Evaluation of microorganisms with sulfidogenic metabolic potential under anaerobic conditions

The aim of this work was to identify groups of microorganisms that are capable of degrading organic matter utilizing sulfate as an electron acceptor. The assay applied for this purpose consisted of running batch reactors and monitoring lactate consumption, sulfate reduction and sulfide production. A portion of the lactate added to the batch reactors was consumed, and the remainder was converted into acetic, propionic and butyric acid after 111 hours of operation These results indicate the presence of sulfate-reducing bacteria (SRB) catalyzing both complete and incomplete oxidation of organic substrates. The sulfate removal efficiency was 49.5% after 1335 hours of operation under an initial sulfate concentration of 1123 mg/L. The SRB concentrations determined by the most probable number (MPN) method were 9.0x10(7) cells/mL at the beginning of the assay and 8.0x10(5) cells/mL after 738 hours of operation.

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Main Authors: Sakamoto,Isabel Kimiko, Maintinguer,Sandra Imaculada, Hirasawa,Julia Sumiko, Adorno,Maria Angela Tallarico, Varesche,Maria Bernadete Amâncio
Format: Digital revista
Language:English
Published: Instituto de Tecnologia do Paraná - Tecpar 2012
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500018
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spelling oai:scielo:S1516-891320120005000182012-10-09Evaluation of microorganisms with sulfidogenic metabolic potential under anaerobic conditionsSakamoto,Isabel KimikoMaintinguer,Sandra ImaculadaHirasawa,Julia SumikoAdorno,Maria Angela TallaricoVaresche,Maria Bernadete Amâncio sulfate-reducing bacteria lactate Most Probable Number organic acids The aim of this work was to identify groups of microorganisms that are capable of degrading organic matter utilizing sulfate as an electron acceptor. The assay applied for this purpose consisted of running batch reactors and monitoring lactate consumption, sulfate reduction and sulfide production. A portion of the lactate added to the batch reactors was consumed, and the remainder was converted into acetic, propionic and butyric acid after 111 hours of operation These results indicate the presence of sulfate-reducing bacteria (SRB) catalyzing both complete and incomplete oxidation of organic substrates. The sulfate removal efficiency was 49.5% after 1335 hours of operation under an initial sulfate concentration of 1123 mg/L. The SRB concentrations determined by the most probable number (MPN) method were 9.0x10(7) cells/mL at the beginning of the assay and 8.0x10(5) cells/mL after 738 hours of operation.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.55 n.5 20122012-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500018en10.1590/S1516-89132012000500018
institution SCIELO
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country Brasil
countrycode BR
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region America del Sur
libraryname SciELO
language English
format Digital
author Sakamoto,Isabel Kimiko
Maintinguer,Sandra Imaculada
Hirasawa,Julia Sumiko
Adorno,Maria Angela Tallarico
Varesche,Maria Bernadete Amâncio
spellingShingle Sakamoto,Isabel Kimiko
Maintinguer,Sandra Imaculada
Hirasawa,Julia Sumiko
Adorno,Maria Angela Tallarico
Varesche,Maria Bernadete Amâncio
Evaluation of microorganisms with sulfidogenic metabolic potential under anaerobic conditions
author_facet Sakamoto,Isabel Kimiko
Maintinguer,Sandra Imaculada
Hirasawa,Julia Sumiko
Adorno,Maria Angela Tallarico
Varesche,Maria Bernadete Amâncio
author_sort Sakamoto,Isabel Kimiko
title Evaluation of microorganisms with sulfidogenic metabolic potential under anaerobic conditions
title_short Evaluation of microorganisms with sulfidogenic metabolic potential under anaerobic conditions
title_full Evaluation of microorganisms with sulfidogenic metabolic potential under anaerobic conditions
title_fullStr Evaluation of microorganisms with sulfidogenic metabolic potential under anaerobic conditions
title_full_unstemmed Evaluation of microorganisms with sulfidogenic metabolic potential under anaerobic conditions
title_sort evaluation of microorganisms with sulfidogenic metabolic potential under anaerobic conditions
description The aim of this work was to identify groups of microorganisms that are capable of degrading organic matter utilizing sulfate as an electron acceptor. The assay applied for this purpose consisted of running batch reactors and monitoring lactate consumption, sulfate reduction and sulfide production. A portion of the lactate added to the batch reactors was consumed, and the remainder was converted into acetic, propionic and butyric acid after 111 hours of operation These results indicate the presence of sulfate-reducing bacteria (SRB) catalyzing both complete and incomplete oxidation of organic substrates. The sulfate removal efficiency was 49.5% after 1335 hours of operation under an initial sulfate concentration of 1123 mg/L. The SRB concentrations determined by the most probable number (MPN) method were 9.0x10(7) cells/mL at the beginning of the assay and 8.0x10(5) cells/mL after 738 hours of operation.
publisher Instituto de Tecnologia do Paraná - Tecpar
publishDate 2012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500018
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AT hirasawajuliasumiko evaluationofmicroorganismswithsulfidogenicmetabolicpotentialunderanaerobicconditions
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