Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats
This study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6) and control (n = 6). After splenectomy (15 days), the administration of 0.1ml of 99mTc-DMSA IV (0.48 MBq) was carried out. Thirty minutes later, kidney, heart, lung, thyroid, stomach, bladder and femur and samples of blood were isolated. The organs were weighed, counted and the percentage of radioactivity /g (%ATI/g) determined. Serum urea and creatinine, hematocrit, leukocytes and platelets were measured. Statistics by t test (p<0.05) was done. There was a significant reduction in %ATI/g in kidney and blood (p<0.05) of splenectomized animals, a significant increase (p<0.05) of urea (88.8 ± 18.6 mg/dL) and creatinine (0.56 ± 0.08 mg/dL), compared to the controls (51.5±1.6, 0.37±0.02mg/dL, respectively), as well as increase in platelets and leucocytes, and hematocrit reduction. The analysis of the results indicates that in rats, splenectomy seems to alter the renal function and the uptake of 99mTc-DMSA.
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Instituto de Tecnologia do Paraná - Tecpar
2008
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oai:scielo:S1516-891320080007000322009-02-20Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in ratsAçucena,Maria Kadja Meneses TorresPereira,Kércia Regina Santos GomesVillarim Neto,ArthurRêgo,Amália Cínthia MenesesBernardo-Filho,MarioAzevedo,Ítalo MedeirosAraújo Filho,IramiMedeiros,Aldo Cunha Splenectomy Biodistribution Technetium Rats This study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6) and control (n = 6). After splenectomy (15 days), the administration of 0.1ml of 99mTc-DMSA IV (0.48 MBq) was carried out. Thirty minutes later, kidney, heart, lung, thyroid, stomach, bladder and femur and samples of blood were isolated. The organs were weighed, counted and the percentage of radioactivity /g (%ATI/g) determined. Serum urea and creatinine, hematocrit, leukocytes and platelets were measured. Statistics by t test (p<0.05) was done. There was a significant reduction in %ATI/g in kidney and blood (p<0.05) of splenectomized animals, a significant increase (p<0.05) of urea (88.8 ± 18.6 mg/dL) and creatinine (0.56 ± 0.08 mg/dL), compared to the controls (51.5±1.6, 0.37±0.02mg/dL, respectively), as well as increase in platelets and leucocytes, and hematocrit reduction. The analysis of the results indicates that in rats, splenectomy seems to alter the renal function and the uptake of 99mTc-DMSA.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.51 n.spe 20082008-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700032en10.1590/S1516-89132008000700032 |
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Açucena,Maria Kadja Meneses Torres Pereira,Kércia Regina Santos Gomes Villarim Neto,Arthur Rêgo,Amália Cínthia Meneses Bernardo-Filho,Mario Azevedo,Ítalo Medeiros Araújo Filho,Irami Medeiros,Aldo Cunha |
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Açucena,Maria Kadja Meneses Torres Pereira,Kércia Regina Santos Gomes Villarim Neto,Arthur Rêgo,Amália Cínthia Meneses Bernardo-Filho,Mario Azevedo,Ítalo Medeiros Araújo Filho,Irami Medeiros,Aldo Cunha Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats |
author_facet |
Açucena,Maria Kadja Meneses Torres Pereira,Kércia Regina Santos Gomes Villarim Neto,Arthur Rêgo,Amália Cínthia Meneses Bernardo-Filho,Mario Azevedo,Ítalo Medeiros Araújo Filho,Irami Medeiros,Aldo Cunha |
author_sort |
Açucena,Maria Kadja Meneses Torres |
title |
Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats |
title_short |
Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats |
title_full |
Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats |
title_fullStr |
Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats |
title_full_unstemmed |
Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats |
title_sort |
influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mtc-dmsa) in rats |
description |
This study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6) and control (n = 6). After splenectomy (15 days), the administration of 0.1ml of 99mTc-DMSA IV (0.48 MBq) was carried out. Thirty minutes later, kidney, heart, lung, thyroid, stomach, bladder and femur and samples of blood were isolated. The organs were weighed, counted and the percentage of radioactivity /g (%ATI/g) determined. Serum urea and creatinine, hematocrit, leukocytes and platelets were measured. Statistics by t test (p<0.05) was done. There was a significant reduction in %ATI/g in kidney and blood (p<0.05) of splenectomized animals, a significant increase (p<0.05) of urea (88.8 ± 18.6 mg/dL) and creatinine (0.56 ± 0.08 mg/dL), compared to the controls (51.5±1.6, 0.37±0.02mg/dL, respectively), as well as increase in platelets and leucocytes, and hematocrit reduction. The analysis of the results indicates that in rats, splenectomy seems to alter the renal function and the uptake of 99mTc-DMSA. |
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Instituto de Tecnologia do Paraná - Tecpar |
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2008 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700032 |
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