An extract of a formula used in the traditional chinese medicine (Buzhong Yi Qi Wan) alters the labeling of blood constituents with technetium-99m
Buzhong Yi Qi Wan (Buzhong) is a medicinal herb widely used in Traditional Chinese Medicine to treat the digestive and circulatory systems. Red blood cell and plasma proteins labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to investigate the effects of an aqueous Buzhong extract on the labeling of blood constituents with 99mTc. Heparinized blood (Wistar rats) was incubated in vitro with different Buzhong extract concentrations and 99mTc-labeling was performed. Plasma (P) and blood cells (BC) were separated and soluble (SF-P, SF-BC) and insoluble (IF-P, IF-BC) fractions were isolated. The radioactivity on blood constituents was determined and the percentage of incorporated radioactivity (%ATI) was calculated. Buzhong extract at the highest concentrations used altered significantly (p<0.05) the %ATI in blood constituents. Substances present in the Buzhong extract could alter the cellular membrane and/or generation of free radicals that have oxidant properties modifying the labeling of blood constituents with 99mTc.
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Instituto de Tecnologia do Paraná - Tecpar
2007
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oai:scielo:S1516-891320070006000132008-03-12An extract of a formula used in the traditional chinese medicine (Buzhong Yi Qi Wan) alters the labeling of blood constituents with technetium-99mGiani,Tania SantosPaoli,Severo dePresta,Giuseppe AntonioMaiworm,Adalgisa IedaSantos-Filho,Sebastião DavidBrandão-Neto,JoséMedeiros,Aldo da CunhaFonseca,Adenilson de Souza daBernardo-Filho,Mario Technetium-99m Red blood cell Oxidant agent Radiobiocomplex Plasma proteins Buzhong Yi Qi Wan Buzhong Yi Qi Wan (Buzhong) is a medicinal herb widely used in Traditional Chinese Medicine to treat the digestive and circulatory systems. Red blood cell and plasma proteins labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to investigate the effects of an aqueous Buzhong extract on the labeling of blood constituents with 99mTc. Heparinized blood (Wistar rats) was incubated in vitro with different Buzhong extract concentrations and 99mTc-labeling was performed. Plasma (P) and blood cells (BC) were separated and soluble (SF-P, SF-BC) and insoluble (IF-P, IF-BC) fractions were isolated. The radioactivity on blood constituents was determined and the percentage of incorporated radioactivity (%ATI) was calculated. Buzhong extract at the highest concentrations used altered significantly (p<0.05) the %ATI in blood constituents. Substances present in the Buzhong extract could alter the cellular membrane and/or generation of free radicals that have oxidant properties modifying the labeling of blood constituents with 99mTc.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.50 n.spe 20072007-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600013en10.1590/S1516-89132007000600013 |
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Giani,Tania Santos Paoli,Severo de Presta,Giuseppe Antonio Maiworm,Adalgisa Ieda Santos-Filho,Sebastião David Brandão-Neto,José Medeiros,Aldo da Cunha Fonseca,Adenilson de Souza da Bernardo-Filho,Mario |
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Giani,Tania Santos Paoli,Severo de Presta,Giuseppe Antonio Maiworm,Adalgisa Ieda Santos-Filho,Sebastião David Brandão-Neto,José Medeiros,Aldo da Cunha Fonseca,Adenilson de Souza da Bernardo-Filho,Mario An extract of a formula used in the traditional chinese medicine (Buzhong Yi Qi Wan) alters the labeling of blood constituents with technetium-99m |
author_facet |
Giani,Tania Santos Paoli,Severo de Presta,Giuseppe Antonio Maiworm,Adalgisa Ieda Santos-Filho,Sebastião David Brandão-Neto,José Medeiros,Aldo da Cunha Fonseca,Adenilson de Souza da Bernardo-Filho,Mario |
author_sort |
Giani,Tania Santos |
title |
An extract of a formula used in the traditional chinese medicine (Buzhong Yi Qi Wan) alters the labeling of blood constituents with technetium-99m |
title_short |
An extract of a formula used in the traditional chinese medicine (Buzhong Yi Qi Wan) alters the labeling of blood constituents with technetium-99m |
title_full |
An extract of a formula used in the traditional chinese medicine (Buzhong Yi Qi Wan) alters the labeling of blood constituents with technetium-99m |
title_fullStr |
An extract of a formula used in the traditional chinese medicine (Buzhong Yi Qi Wan) alters the labeling of blood constituents with technetium-99m |
title_full_unstemmed |
An extract of a formula used in the traditional chinese medicine (Buzhong Yi Qi Wan) alters the labeling of blood constituents with technetium-99m |
title_sort |
extract of a formula used in the traditional chinese medicine (buzhong yi qi wan) alters the labeling of blood constituents with technetium-99m |
description |
Buzhong Yi Qi Wan (Buzhong) is a medicinal herb widely used in Traditional Chinese Medicine to treat the digestive and circulatory systems. Red blood cell and plasma proteins labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to investigate the effects of an aqueous Buzhong extract on the labeling of blood constituents with 99mTc. Heparinized blood (Wistar rats) was incubated in vitro with different Buzhong extract concentrations and 99mTc-labeling was performed. Plasma (P) and blood cells (BC) were separated and soluble (SF-P, SF-BC) and insoluble (IF-P, IF-BC) fractions were isolated. The radioactivity on blood constituents was determined and the percentage of incorporated radioactivity (%ATI) was calculated. Buzhong extract at the highest concentrations used altered significantly (p<0.05) the %ATI in blood constituents. Substances present in the Buzhong extract could alter the cellular membrane and/or generation of free radicals that have oxidant properties modifying the labeling of blood constituents with 99mTc. |
publisher |
Instituto de Tecnologia do Paraná - Tecpar |
publishDate |
2007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600013 |
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