Use of chitosan gel for the purification of protein
The aim of this study was to optimize the preparation of chitosan gel and to use it for protein purification. The optimized preparation parameters were chitosan concentration 2.0%, glutaraldehyde concentration 0.6%, low cross-linking rate, NaOH concentration 1.6%, amount of NaBH4 0.4 g. In order to use the chitosan gel, the elution conditions were optimized as follows. NaCl concentration 0.05 mol/L in the tris-HCl (pH 9.05) at the flow rate of 2.03.0 mL/min. Particle size of chitosan gel was 120-140 µm. Neutral protease could be separated into four ingredients through chitosan gel column. The yield of enzyme was more than 90%. Albumin bovine serum could be separated into two ingredients through gel column and the total yield of albumin bovine serum was more than 70%.
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Instituto de Tecnologia do Paraná - Tecpar
2007
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oai:scielo:S1516-891320070002000152007-06-12Use of chitosan gel for the purification of proteinTang,ZhenXingQian,JunQing Chitosan Gel Preparation Application The aim of this study was to optimize the preparation of chitosan gel and to use it for protein purification. The optimized preparation parameters were chitosan concentration 2.0%, glutaraldehyde concentration 0.6%, low cross-linking rate, NaOH concentration 1.6%, amount of NaBH4 0.4 g. In order to use the chitosan gel, the elution conditions were optimized as follows. NaCl concentration 0.05 mol/L in the tris-HCl (pH 9.05) at the flow rate of 2.03.0 mL/min. Particle size of chitosan gel was 120-140 µm. Neutral protease could be separated into four ingredients through chitosan gel column. The yield of enzyme was more than 90%. Albumin bovine serum could be separated into two ingredients through gel column and the total yield of albumin bovine serum was more than 70%.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.50 n.2 20072007-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200015en10.1590/S1516-89132007000200015 |
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Tang,ZhenXing Qian,JunQing |
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Tang,ZhenXing Qian,JunQing Use of chitosan gel for the purification of protein |
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Tang,ZhenXing Qian,JunQing |
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Tang,ZhenXing |
| title |
Use of chitosan gel for the purification of protein |
| title_short |
Use of chitosan gel for the purification of protein |
| title_full |
Use of chitosan gel for the purification of protein |
| title_fullStr |
Use of chitosan gel for the purification of protein |
| title_full_unstemmed |
Use of chitosan gel for the purification of protein |
| title_sort |
use of chitosan gel for the purification of protein |
| description |
The aim of this study was to optimize the preparation of chitosan gel and to use it for protein purification. The optimized preparation parameters were chitosan concentration 2.0%, glutaraldehyde concentration 0.6%, low cross-linking rate, NaOH concentration 1.6%, amount of NaBH4 0.4 g. In order to use the chitosan gel, the elution conditions were optimized as follows. NaCl concentration 0.05 mol/L in the tris-HCl (pH 9.05) at the flow rate of 2.03.0 mL/min. Particle size of chitosan gel was 120-140 µm. Neutral protease could be separated into four ingredients through chitosan gel column. The yield of enzyme was more than 90%. Albumin bovine serum could be separated into two ingredients through gel column and the total yield of albumin bovine serum was more than 70%. |
| publisher |
Instituto de Tecnologia do Paraná - Tecpar |
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2007 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200015 |
| work_keys_str_mv |
AT tangzhenxing useofchitosangelforthepurificationofprotein AT qianjunqing useofchitosangelforthepurificationofprotein |
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1756423753650143232 |