Biodistribution of free 99mTc-ovalbumin and 99mTc-ovalbumin encapsulated in liposomes
The oral administration of proteic antigens, like ovalbumin, may result in the induction of oral tolerance or immunization. The aim of this work was to label a protein antigen with 99mTechnetium, encapsulate it in liposomes and investigate its absorption and tissue distribution after oral administration in mice. Ovalbumin was labeled with 99mTechnetium and encapsulated in small unilamellar vesicles. 99mTc-OVA encapsulated or not in liposomes was administrated to mice that were sacrificed after different times. The radioactivity was measured in various organs of the animals. Differences concerning the biodistribution of 99mTc-OVA were noticed. The technique may represent alternatives for the induction of immunization or oral tolerance.
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Instituto de Tecnologia do Paraná - Tecpar
2005
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oai:scielo:S1516-891320050007000352005-12-09Biodistribution of free 99mTc-ovalbumin and 99mTc-ovalbumin encapsulated in liposomesAlves,Andrea CatãoGontijo,Cristiano MachadoOliveira,Mônica CristinaDiniz,Simone Odília FernandesOliveira,Flávia MárciaCardoso,Valbert NascimentoRamaldes,Gilson Andrade Liposomes oral absorption mucosal delivery proteins nanotechnology The oral administration of proteic antigens, like ovalbumin, may result in the induction of oral tolerance or immunization. The aim of this work was to label a protein antigen with 99mTechnetium, encapsulate it in liposomes and investigate its absorption and tissue distribution after oral administration in mice. Ovalbumin was labeled with 99mTechnetium and encapsulated in small unilamellar vesicles. 99mTc-OVA encapsulated or not in liposomes was administrated to mice that were sacrificed after different times. The radioactivity was measured in various organs of the animals. Differences concerning the biodistribution of 99mTc-OVA were noticed. The technique may represent alternatives for the induction of immunization or oral tolerance.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.48 n.spe2 20052005-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700035en10.1590/S1516-89132005000700035 |
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Alves,Andrea Catão Gontijo,Cristiano Machado Oliveira,Mônica Cristina Diniz,Simone Odília Fernandes Oliveira,Flávia Márcia Cardoso,Valbert Nascimento Ramaldes,Gilson Andrade |
| spellingShingle |
Alves,Andrea Catão Gontijo,Cristiano Machado Oliveira,Mônica Cristina Diniz,Simone Odília Fernandes Oliveira,Flávia Márcia Cardoso,Valbert Nascimento Ramaldes,Gilson Andrade Biodistribution of free 99mTc-ovalbumin and 99mTc-ovalbumin encapsulated in liposomes |
| author_facet |
Alves,Andrea Catão Gontijo,Cristiano Machado Oliveira,Mônica Cristina Diniz,Simone Odília Fernandes Oliveira,Flávia Márcia Cardoso,Valbert Nascimento Ramaldes,Gilson Andrade |
| author_sort |
Alves,Andrea Catão |
| title |
Biodistribution of free 99mTc-ovalbumin and 99mTc-ovalbumin encapsulated in liposomes |
| title_short |
Biodistribution of free 99mTc-ovalbumin and 99mTc-ovalbumin encapsulated in liposomes |
| title_full |
Biodistribution of free 99mTc-ovalbumin and 99mTc-ovalbumin encapsulated in liposomes |
| title_fullStr |
Biodistribution of free 99mTc-ovalbumin and 99mTc-ovalbumin encapsulated in liposomes |
| title_full_unstemmed |
Biodistribution of free 99mTc-ovalbumin and 99mTc-ovalbumin encapsulated in liposomes |
| title_sort |
biodistribution of free 99mtc-ovalbumin and 99mtc-ovalbumin encapsulated in liposomes |
| description |
The oral administration of proteic antigens, like ovalbumin, may result in the induction of oral tolerance or immunization. The aim of this work was to label a protein antigen with 99mTechnetium, encapsulate it in liposomes and investigate its absorption and tissue distribution after oral administration in mice. Ovalbumin was labeled with 99mTechnetium and encapsulated in small unilamellar vesicles. 99mTc-OVA encapsulated or not in liposomes was administrated to mice that were sacrificed after different times. The radioactivity was measured in various organs of the animals. Differences concerning the biodistribution of 99mTc-OVA were noticed. The technique may represent alternatives for the induction of immunization or oral tolerance. |
| publisher |
Instituto de Tecnologia do Paraná - Tecpar |
| publishDate |
2005 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700035 |
| work_keys_str_mv |
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