The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast Cells
ABSTRACT The effects of oxidative stress induced by high temperature on the cell viability, proliferation, apoptosis and oxidative status of chicken embryonic fibroblasts (CEF) were analyzed. The viability, proliferation, apoptotic and anti-oxidative status were measured after incubating CEF at the temperatures of 37ºC (control) and 40-44ºC (experimental groups) for 6,12 and 24 hours. The results showed that at high temperature (42-43ºC), the viability of CEF cells decreased after 6, 12 and 24 h of incubation, but the difference was significant only at 43ºC. Cell proliferation was significantly reduced at 44oC/6h. The apoptotic rate of CEF cells was increased following heat treatments in a time-dependent manner. ROS formation increased with increasing temperature, but the difference was only significant at 44ºC/6,12h. Heat stress did not significantly affect the superoxide dismutase (SOD) activity. CAT activity was significantly decreased at 43ºC/24h and 44ºC/12 and 24h. Malondialdehyde (MDA) formation was significantly increased at 43ºC/12h and 44ºC/12 and 24h. In conclusion, heat stress induced the oxidative stress, decreasing the viability, proliferation and anti-oxidative response of CEF cells.
Main Authors: | , , , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Fundacao de Apoio a Ciência e Tecnologia Avicolas
2018
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300463 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S1516-635X2018000300463 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S1516-635X20180003004632018-09-20The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast CellsIbtisham,FZhao,YNawab,ALiguang,HWu,JXiao,MZhao,ZAn,L Apoptosis Chicken embryonic fibroblast Cell proliferation Heat stress Oxidative stress ABSTRACT The effects of oxidative stress induced by high temperature on the cell viability, proliferation, apoptosis and oxidative status of chicken embryonic fibroblasts (CEF) were analyzed. The viability, proliferation, apoptotic and anti-oxidative status were measured after incubating CEF at the temperatures of 37ºC (control) and 40-44ºC (experimental groups) for 6,12 and 24 hours. The results showed that at high temperature (42-43ºC), the viability of CEF cells decreased after 6, 12 and 24 h of incubation, but the difference was significant only at 43ºC. Cell proliferation was significantly reduced at 44oC/6h. The apoptotic rate of CEF cells was increased following heat treatments in a time-dependent manner. ROS formation increased with increasing temperature, but the difference was only significant at 44ºC/6,12h. Heat stress did not significantly affect the superoxide dismutase (SOD) activity. CAT activity was significantly decreased at 43ºC/24h and 44ºC/12 and 24h. Malondialdehyde (MDA) formation was significantly increased at 43ºC/12h and 44ºC/12 and 24h. In conclusion, heat stress induced the oxidative stress, decreasing the viability, proliferation and anti-oxidative response of CEF cells.info:eu-repo/semantics/openAccessFundacao de Apoio a Ciência e Tecnologia AvicolasBrazilian Journal of Poultry Science v.20 n.3 20182018-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300463en10.1590/1806-9061-2017-0685 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Ibtisham,F Zhao,Y Nawab,A Liguang,H Wu,J Xiao,M Zhao,Z An,L |
spellingShingle |
Ibtisham,F Zhao,Y Nawab,A Liguang,H Wu,J Xiao,M Zhao,Z An,L The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast Cells |
author_facet |
Ibtisham,F Zhao,Y Nawab,A Liguang,H Wu,J Xiao,M Zhao,Z An,L |
author_sort |
Ibtisham,F |
title |
The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast Cells |
title_short |
The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast Cells |
title_full |
The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast Cells |
title_fullStr |
The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast Cells |
title_full_unstemmed |
The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast Cells |
title_sort |
effect of high temperature on viability, proliferation, apoptosis and anti-oxidant status of chicken embryonic fibroblast cells |
description |
ABSTRACT The effects of oxidative stress induced by high temperature on the cell viability, proliferation, apoptosis and oxidative status of chicken embryonic fibroblasts (CEF) were analyzed. The viability, proliferation, apoptotic and anti-oxidative status were measured after incubating CEF at the temperatures of 37ºC (control) and 40-44ºC (experimental groups) for 6,12 and 24 hours. The results showed that at high temperature (42-43ºC), the viability of CEF cells decreased after 6, 12 and 24 h of incubation, but the difference was significant only at 43ºC. Cell proliferation was significantly reduced at 44oC/6h. The apoptotic rate of CEF cells was increased following heat treatments in a time-dependent manner. ROS formation increased with increasing temperature, but the difference was only significant at 44ºC/6,12h. Heat stress did not significantly affect the superoxide dismutase (SOD) activity. CAT activity was significantly decreased at 43ºC/24h and 44ºC/12 and 24h. Malondialdehyde (MDA) formation was significantly increased at 43ºC/12h and 44ºC/12 and 24h. In conclusion, heat stress induced the oxidative stress, decreasing the viability, proliferation and anti-oxidative response of CEF cells. |
publisher |
Fundacao de Apoio a Ciência e Tecnologia Avicolas |
publishDate |
2018 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300463 |
work_keys_str_mv |
AT ibtishamf theeffectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells AT zhaoy theeffectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells AT nawaba theeffectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells AT liguangh theeffectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells AT wuj theeffectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells AT xiaom theeffectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells AT zhaoz theeffectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells AT anl theeffectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells AT ibtishamf effectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells AT zhaoy effectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells AT nawaba effectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells AT liguangh effectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells AT wuj effectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells AT xiaom effectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells AT zhaoz effectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells AT anl effectofhightemperatureonviabilityproliferationapoptosisandantioxidantstatusofchickenembryonicfibroblastcells |
_version_ |
1756423072814989312 |