Methionine + cystine levels and vitamin B6 supplementation on performance and enzyme expression of methionine metabolism of gilts from 75 to 100 kg

ABSTRACT This study was carried out to evaluate the effect of different levels of standardized ileal digestible (SID) methionine + cystine (Met+Cys) and vitamin B6 supplementation on the performance, blood variables, and gene expression of enzymes involved in methionine metabolism in female pigs between 75 and 100 kg. Fifty six female pigs were used (Talent × Topigs 20), averaging 75.06±1.68 kg in initial weight, allotted in a completely randomized block design arranged in a 2 × 4 factorial scheme, composed of two vitamin B6 supplementation levels (1.58 and 3.58 mg/kg) and four levels of SID Met+Cys (0.370, 0.470, 0.570, and 0.670%), with seven replicates and one animal per experimental unit. No interactions between vitamin B6 supplementation and SID Met+Cys levels were observed. The levels of SID Met+Cys and vitamin B6 supplementation did not affect animal performance. Triacylglycerols showed a quadratic response to the SID Met+Cys levels, in which the lowest plasma concentration was estimated as 0.575%. Treatments did not affect the expression of the methionine synthase and cystathionine-γ-lyase enzymes or serum homocysteine levels. The SID Met+Cys requirement for female pigs from 75 to 100 kg is equal to or lower than 10.60 g/day, which corresponds to the level of 0.370% Met+Cys in the diet and a relationship 0.48% with the SID lysine.

Saved in:
Bibliographic Details
Main Authors: Sangali,Cleiton Pagliari, Gasparino,Eliane, Vasconcellos,Ricardo Souza, Fachinello,Marcelise Regina, Monteiro,Alessandra Nardina Trícia Rigo, Esteves,Lucas Antonio Costa, Bonagurio,Lucas Pimentel, Pozza,Paulo Cesar
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Zootecnia 2017
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000300223
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S1516-35982017000300223
record_format ojs
spelling oai:scielo:S1516-359820170003002232017-05-05Methionine + cystine levels and vitamin B6 supplementation on performance and enzyme expression of methionine metabolism of gilts from 75 to 100 kgSangali,Cleiton PagliariGasparino,ElianeVasconcellos,Ricardo SouzaFachinello,Marcelise ReginaMonteiro,Alessandra Nardina Trícia RigoEsteves,Lucas Antonio CostaBonagurio,Lucas PimentelPozza,Paulo Cesar blood variable cystathionine-γ-lyase homocysteine methionine synthase performance pigs ABSTRACT This study was carried out to evaluate the effect of different levels of standardized ileal digestible (SID) methionine + cystine (Met+Cys) and vitamin B6 supplementation on the performance, blood variables, and gene expression of enzymes involved in methionine metabolism in female pigs between 75 and 100 kg. Fifty six female pigs were used (Talent × Topigs 20), averaging 75.06±1.68 kg in initial weight, allotted in a completely randomized block design arranged in a 2 × 4 factorial scheme, composed of two vitamin B6 supplementation levels (1.58 and 3.58 mg/kg) and four levels of SID Met+Cys (0.370, 0.470, 0.570, and 0.670%), with seven replicates and one animal per experimental unit. No interactions between vitamin B6 supplementation and SID Met+Cys levels were observed. The levels of SID Met+Cys and vitamin B6 supplementation did not affect animal performance. Triacylglycerols showed a quadratic response to the SID Met+Cys levels, in which the lowest plasma concentration was estimated as 0.575%. Treatments did not affect the expression of the methionine synthase and cystathionine-γ-lyase enzymes or serum homocysteine levels. The SID Met+Cys requirement for female pigs from 75 to 100 kg is equal to or lower than 10.60 g/day, which corresponds to the level of 0.370% Met+Cys in the diet and a relationship 0.48% with the SID lysine.info:eu-repo/semantics/openAccessSociedade Brasileira de ZootecniaRevista Brasileira de Zootecnia v.46 n.3 20172017-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000300223en10.1590/s1806-92902017000300007
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Sangali,Cleiton Pagliari
Gasparino,Eliane
Vasconcellos,Ricardo Souza
Fachinello,Marcelise Regina
Monteiro,Alessandra Nardina Trícia Rigo
Esteves,Lucas Antonio Costa
Bonagurio,Lucas Pimentel
Pozza,Paulo Cesar
spellingShingle Sangali,Cleiton Pagliari
Gasparino,Eliane
Vasconcellos,Ricardo Souza
Fachinello,Marcelise Regina
Monteiro,Alessandra Nardina Trícia Rigo
Esteves,Lucas Antonio Costa
Bonagurio,Lucas Pimentel
Pozza,Paulo Cesar
Methionine + cystine levels and vitamin B6 supplementation on performance and enzyme expression of methionine metabolism of gilts from 75 to 100 kg
author_facet Sangali,Cleiton Pagliari
Gasparino,Eliane
Vasconcellos,Ricardo Souza
Fachinello,Marcelise Regina
Monteiro,Alessandra Nardina Trícia Rigo
Esteves,Lucas Antonio Costa
Bonagurio,Lucas Pimentel
Pozza,Paulo Cesar
author_sort Sangali,Cleiton Pagliari
title Methionine + cystine levels and vitamin B6 supplementation on performance and enzyme expression of methionine metabolism of gilts from 75 to 100 kg
title_short Methionine + cystine levels and vitamin B6 supplementation on performance and enzyme expression of methionine metabolism of gilts from 75 to 100 kg
title_full Methionine + cystine levels and vitamin B6 supplementation on performance and enzyme expression of methionine metabolism of gilts from 75 to 100 kg
title_fullStr Methionine + cystine levels and vitamin B6 supplementation on performance and enzyme expression of methionine metabolism of gilts from 75 to 100 kg
title_full_unstemmed Methionine + cystine levels and vitamin B6 supplementation on performance and enzyme expression of methionine metabolism of gilts from 75 to 100 kg
title_sort methionine + cystine levels and vitamin b6 supplementation on performance and enzyme expression of methionine metabolism of gilts from 75 to 100 kg
description ABSTRACT This study was carried out to evaluate the effect of different levels of standardized ileal digestible (SID) methionine + cystine (Met+Cys) and vitamin B6 supplementation on the performance, blood variables, and gene expression of enzymes involved in methionine metabolism in female pigs between 75 and 100 kg. Fifty six female pigs were used (Talent × Topigs 20), averaging 75.06±1.68 kg in initial weight, allotted in a completely randomized block design arranged in a 2 × 4 factorial scheme, composed of two vitamin B6 supplementation levels (1.58 and 3.58 mg/kg) and four levels of SID Met+Cys (0.370, 0.470, 0.570, and 0.670%), with seven replicates and one animal per experimental unit. No interactions between vitamin B6 supplementation and SID Met+Cys levels were observed. The levels of SID Met+Cys and vitamin B6 supplementation did not affect animal performance. Triacylglycerols showed a quadratic response to the SID Met+Cys levels, in which the lowest plasma concentration was estimated as 0.575%. Treatments did not affect the expression of the methionine synthase and cystathionine-γ-lyase enzymes or serum homocysteine levels. The SID Met+Cys requirement for female pigs from 75 to 100 kg is equal to or lower than 10.60 g/day, which corresponds to the level of 0.370% Met+Cys in the diet and a relationship 0.48% with the SID lysine.
publisher Sociedade Brasileira de Zootecnia
publishDate 2017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000300223
work_keys_str_mv AT sangalicleitonpagliari methioninecystinelevelsandvitaminb6supplementationonperformanceandenzymeexpressionofmethioninemetabolismofgiltsfrom75to100kg
AT gasparinoeliane methioninecystinelevelsandvitaminb6supplementationonperformanceandenzymeexpressionofmethioninemetabolismofgiltsfrom75to100kg
AT vasconcellosricardosouza methioninecystinelevelsandvitaminb6supplementationonperformanceandenzymeexpressionofmethioninemetabolismofgiltsfrom75to100kg
AT fachinellomarceliseregina methioninecystinelevelsandvitaminb6supplementationonperformanceandenzymeexpressionofmethioninemetabolismofgiltsfrom75to100kg
AT monteiroalessandranardinatriciarigo methioninecystinelevelsandvitaminb6supplementationonperformanceandenzymeexpressionofmethioninemetabolismofgiltsfrom75to100kg
AT esteveslucasantoniocosta methioninecystinelevelsandvitaminb6supplementationonperformanceandenzymeexpressionofmethioninemetabolismofgiltsfrom75to100kg
AT bonaguriolucaspimentel methioninecystinelevelsandvitaminb6supplementationonperformanceandenzymeexpressionofmethioninemetabolismofgiltsfrom75to100kg
AT pozzapaulocesar methioninecystinelevelsandvitaminb6supplementationonperformanceandenzymeexpressionofmethioninemetabolismofgiltsfrom75to100kg
_version_ 1756422423643684864