Genetic diversity of four cattle breeds using microsatellite markers
Four cattle breeds (Gyr, Nellore, Guzerat and Holstein) were analyzed by amplification of genomic DNA using microsatellite loci to evaluate the genetic diversity within and among them. DNA samples of 18 animals from each breed were collected to access the genetic content of them. Allele frequencies were calculated and used to generate a Nei's genetic distance matrix what was used to build a dendrogram following UPGMA clustering. As expected, Holstein breed was the most distinct from the other breeds: 1.15 in relation to Gyr, 1.12 in relation to Nellore and 0.94 in relation to Guzerat. The closest genetic distance was 0.25 between Guzerat and Nellore. A total of 64 alleles in all four breeds were detected using nine microsatellite primers. Each breed showed 53% of the total number of alleles. The average number of alleles per locus was 7.11 ± 3.21. The most informative locus was BMS1237 with 53% of observed heterozygosity and the least informative locus was BMS3004 with 12% only. The average heterozygosity detected for the nine loci were 35% and the expected value for Hardy-Weinberg equilibrium was 53%. This low heterozygosity suggests a high endogamy level among the animals sampled within each breed.
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Sociedade Brasileira de Zootecnia
2003
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oai:scielo:S1516-359820030001000122003-07-04Genetic diversity of four cattle breeds using microsatellite markersMachado,Marco AntonioSchuster,IvanMartinez,Mário LuizCampos,Ana Lúcia bovine DNA genetic diversity marker microsatellite Four cattle breeds (Gyr, Nellore, Guzerat and Holstein) were analyzed by amplification of genomic DNA using microsatellite loci to evaluate the genetic diversity within and among them. DNA samples of 18 animals from each breed were collected to access the genetic content of them. Allele frequencies were calculated and used to generate a Nei's genetic distance matrix what was used to build a dendrogram following UPGMA clustering. As expected, Holstein breed was the most distinct from the other breeds: 1.15 in relation to Gyr, 1.12 in relation to Nellore and 0.94 in relation to Guzerat. The closest genetic distance was 0.25 between Guzerat and Nellore. A total of 64 alleles in all four breeds were detected using nine microsatellite primers. Each breed showed 53% of the total number of alleles. The average number of alleles per locus was 7.11 ± 3.21. The most informative locus was BMS1237 with 53% of observed heterozygosity and the least informative locus was BMS3004 with 12% only. The average heterozygosity detected for the nine loci were 35% and the expected value for Hardy-Weinberg equilibrium was 53%. This low heterozygosity suggests a high endogamy level among the animals sampled within each breed.info:eu-repo/semantics/openAccessSociedade Brasileira de ZootecniaRevista Brasileira de Zootecnia v.32 n.1 20032003-02-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982003000100012en10.1590/S1516-35982003000100012 |
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Machado,Marco Antonio Schuster,Ivan Martinez,Mário Luiz Campos,Ana Lúcia |
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Machado,Marco Antonio Schuster,Ivan Martinez,Mário Luiz Campos,Ana Lúcia Genetic diversity of four cattle breeds using microsatellite markers |
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Machado,Marco Antonio Schuster,Ivan Martinez,Mário Luiz Campos,Ana Lúcia |
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Machado,Marco Antonio |
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Genetic diversity of four cattle breeds using microsatellite markers |
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Genetic diversity of four cattle breeds using microsatellite markers |
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Genetic diversity of four cattle breeds using microsatellite markers |
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Genetic diversity of four cattle breeds using microsatellite markers |
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Genetic diversity of four cattle breeds using microsatellite markers |
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genetic diversity of four cattle breeds using microsatellite markers |
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Four cattle breeds (Gyr, Nellore, Guzerat and Holstein) were analyzed by amplification of genomic DNA using microsatellite loci to evaluate the genetic diversity within and among them. DNA samples of 18 animals from each breed were collected to access the genetic content of them. Allele frequencies were calculated and used to generate a Nei's genetic distance matrix what was used to build a dendrogram following UPGMA clustering. As expected, Holstein breed was the most distinct from the other breeds: 1.15 in relation to Gyr, 1.12 in relation to Nellore and 0.94 in relation to Guzerat. The closest genetic distance was 0.25 between Guzerat and Nellore. A total of 64 alleles in all four breeds were detected using nine microsatellite primers. Each breed showed 53% of the total number of alleles. The average number of alleles per locus was 7.11 ± 3.21. The most informative locus was BMS1237 with 53% of observed heterozygosity and the least informative locus was BMS3004 with 12% only. The average heterozygosity detected for the nine loci were 35% and the expected value for Hardy-Weinberg equilibrium was 53%. This low heterozygosity suggests a high endogamy level among the animals sampled within each breed. |
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Sociedade Brasileira de Zootecnia |
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2003 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982003000100012 |
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