Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)

Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)

Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-submetacentric chromosomes. The sex determination mechanism was of the Xy p type in D. nisus and D. semisquamosus and of the Xyr type in D. sericeus. C-banding revealed the presence of pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of the three species. After silver staining, the nucleolar organizer regions (NORs) were located in autosomes of D. semisquamosus and D. sericeus and in the sexual bivalent of D. nisus. FISH with an rDNA probe confirmed NORs location in D. semisquamosus and in D. nisus. Our results suggest that chromosome inversions and fusions occurred during the evolution of the group.

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Main Authors: Silva,Guilherme Messias da, Bione,Edgar Guimarães, Cabral-de-Mello,Diogo Cavalcanti, Moura,Rita de Cássia de, Simões,Zilá Luz Paulino, Souza,Maria José de
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Genética 2009
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200012
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spelling oai:scielo:S1415-475720090002000122009-05-25Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)Silva,Guilherme Messias daBione,Edgar GuimarãesCabral-de-Mello,Diogo CavalcantiMoura,Rita de Cássia deSimões,Zilá Luz PaulinoSouza,Maria José de chromosome rearrangements heterochromatin karyotype NORs FISH Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-submetacentric chromosomes. The sex determination mechanism was of the Xy p type in D. nisus and D. semisquamosus and of the Xyr type in D. sericeus. C-banding revealed the presence of pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of the three species. After silver staining, the nucleolar organizer regions (NORs) were located in autosomes of D. semisquamosus and D. sericeus and in the sexual bivalent of D. nisus. FISH with an rDNA probe confirmed NORs location in D. semisquamosus and in D. nisus. Our results suggest that chromosome inversions and fusions occurred during the evolution of the group.info:eu-repo/semantics/openAccessSociedade Brasileira de GenéticaGenetics and Molecular Biology v.32 n.2 20092009-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200012en10.1590/S1415-47572009005000040
institution SCIELO
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country Brasil
countrycode BR
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databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Silva,Guilherme Messias da
Bione,Edgar Guimarães
Cabral-de-Mello,Diogo Cavalcanti
Moura,Rita de Cássia de
Simões,Zilá Luz Paulino
Souza,Maria José de
spellingShingle Silva,Guilherme Messias da
Bione,Edgar Guimarães
Cabral-de-Mello,Diogo Cavalcanti
Moura,Rita de Cássia de
Simões,Zilá Luz Paulino
Souza,Maria José de
Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
author_facet Silva,Guilherme Messias da
Bione,Edgar Guimarães
Cabral-de-Mello,Diogo Cavalcanti
Moura,Rita de Cássia de
Simões,Zilá Luz Paulino
Souza,Maria José de
author_sort Silva,Guilherme Messias da
title Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
title_short Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
title_full Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
title_fullStr Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
title_full_unstemmed Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)
title_sort comparative cytogenetics of three species of dichotomius (coleoptera, scarabaeidae)
description Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-submetacentric chromosomes. The sex determination mechanism was of the Xy p type in D. nisus and D. semisquamosus and of the Xyr type in D. sericeus. C-banding revealed the presence of pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of the three species. After silver staining, the nucleolar organizer regions (NORs) were located in autosomes of D. semisquamosus and D. sericeus and in the sexual bivalent of D. nisus. FISH with an rDNA probe confirmed NORs location in D. semisquamosus and in D. nisus. Our results suggest that chromosome inversions and fusions occurred during the evolution of the group.
publisher Sociedade Brasileira de Genética
publishDate 2009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200012
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