Influence of Irrigants in Association with Ultrasound for Cleaning Root Dentin Surface after Post Space Preparation: EDS Analysis

This study evaluated the cleanliness of post space after different irrigation protocols, associated or not to the use of ultrasound. Thirty-six single-rooted bovine roots were instrumented with rotary system, irrigated with 1 % hypochlorite and filled with Ah Plus sealer and gutta-percha cones. After seven days in stove at 37 °C, two-thirds of canal filling was removed by drilling and the post space preparation was irrigated with 2.5 % NaOCl (group NaOCl); 0.2 % chlorhexidine (group CLX); distilled water (group Water); 2.5 % NaOCl + ultrasound (group NaOCl-US); 0.2 % chlorhexidine + US (Group CLX-US); or water + US (Group Water-US). Roots were cleaved in mesiodistal direction, prepared for scanning electron microscope (SEM) to evaluate the smear layer and debris presence and for energy dispersive spectroscopy (EDS) analyses, to evaluate chemical components of dentin after treatment. The data were subjected to statistical analysis (two-way Anova and Tukey's test 5 %). EDS analysis showed the presence of chemical components of filling material in the samples from all groups. SEM images showed large amount of obliterated dentinal tubules. The use of ultrasound influenced the removal of some components of filling material (p<0.05), but not allowed for total cleaning. It was concluded that none of the irrigating solutions, associated or not to the use of ultrasound were able to completely clean the post space.

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Main Authors: Junqueira,Rafael Binato, Vasconcelos,Rafaela Andrade de, Bresciani,Eduardo, Marinho,Renata Marques de Melo, Valera,Marcia Carneiro
Format: Digital revista
Language:English
Published: Universidad de La Frontera. Facultad de Medicina 2016
Online Access:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2016000100007
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spelling oai:scielo:S0718-381X20160001000072016-05-23Influence of Irrigants in Association with Ultrasound for Cleaning Root Dentin Surface after Post Space Preparation: EDS AnalysisJunqueira,Rafael BinatoVasconcelos,Rafaela Andrade deBresciani,EduardoMarinho,Renata Marques de MeloValera,Marcia Carneiro endodontics energy dispersive spectroscopy irrigation post space preparation This study evaluated the cleanliness of post space after different irrigation protocols, associated or not to the use of ultrasound. Thirty-six single-rooted bovine roots were instrumented with rotary system, irrigated with 1 % hypochlorite and filled with Ah Plus sealer and gutta-percha cones. After seven days in stove at 37 °C, two-thirds of canal filling was removed by drilling and the post space preparation was irrigated with 2.5 % NaOCl (group NaOCl); 0.2 % chlorhexidine (group CLX); distilled water (group Water); 2.5 % NaOCl + ultrasound (group NaOCl-US); 0.2 % chlorhexidine + US (Group CLX-US); or water + US (Group Water-US). Roots were cleaved in mesiodistal direction, prepared for scanning electron microscope (SEM) to evaluate the smear layer and debris presence and for energy dispersive spectroscopy (EDS) analyses, to evaluate chemical components of dentin after treatment. The data were subjected to statistical analysis (two-way Anova and Tukey's test 5 %). EDS analysis showed the presence of chemical components of filling material in the samples from all groups. SEM images showed large amount of obliterated dentinal tubules. The use of ultrasound influenced the removal of some components of filling material (p<0.05), but not allowed for total cleaning. It was concluded that none of the irrigating solutions, associated or not to the use of ultrasound were able to completely clean the post space.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.10 n.1 20162016-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2016000100007en10.4067/S0718-381X2016000100007
institution SCIELO
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country Chile
countrycode CL
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databasecode rev-scielo-cl
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region America del Sur
libraryname SciELO
language English
format Digital
author Junqueira,Rafael Binato
Vasconcelos,Rafaela Andrade de
Bresciani,Eduardo
Marinho,Renata Marques de Melo
Valera,Marcia Carneiro
spellingShingle Junqueira,Rafael Binato
Vasconcelos,Rafaela Andrade de
Bresciani,Eduardo
Marinho,Renata Marques de Melo
Valera,Marcia Carneiro
Influence of Irrigants in Association with Ultrasound for Cleaning Root Dentin Surface after Post Space Preparation: EDS Analysis
author_facet Junqueira,Rafael Binato
Vasconcelos,Rafaela Andrade de
Bresciani,Eduardo
Marinho,Renata Marques de Melo
Valera,Marcia Carneiro
author_sort Junqueira,Rafael Binato
title Influence of Irrigants in Association with Ultrasound for Cleaning Root Dentin Surface after Post Space Preparation: EDS Analysis
title_short Influence of Irrigants in Association with Ultrasound for Cleaning Root Dentin Surface after Post Space Preparation: EDS Analysis
title_full Influence of Irrigants in Association with Ultrasound for Cleaning Root Dentin Surface after Post Space Preparation: EDS Analysis
title_fullStr Influence of Irrigants in Association with Ultrasound for Cleaning Root Dentin Surface after Post Space Preparation: EDS Analysis
title_full_unstemmed Influence of Irrigants in Association with Ultrasound for Cleaning Root Dentin Surface after Post Space Preparation: EDS Analysis
title_sort influence of irrigants in association with ultrasound for cleaning root dentin surface after post space preparation: eds analysis
description This study evaluated the cleanliness of post space after different irrigation protocols, associated or not to the use of ultrasound. Thirty-six single-rooted bovine roots were instrumented with rotary system, irrigated with 1 % hypochlorite and filled with Ah Plus sealer and gutta-percha cones. After seven days in stove at 37 °C, two-thirds of canal filling was removed by drilling and the post space preparation was irrigated with 2.5 % NaOCl (group NaOCl); 0.2 % chlorhexidine (group CLX); distilled water (group Water); 2.5 % NaOCl + ultrasound (group NaOCl-US); 0.2 % chlorhexidine + US (Group CLX-US); or water + US (Group Water-US). Roots were cleaved in mesiodistal direction, prepared for scanning electron microscope (SEM) to evaluate the smear layer and debris presence and for energy dispersive spectroscopy (EDS) analyses, to evaluate chemical components of dentin after treatment. The data were subjected to statistical analysis (two-way Anova and Tukey's test 5 %). EDS analysis showed the presence of chemical components of filling material in the samples from all groups. SEM images showed large amount of obliterated dentinal tubules. The use of ultrasound influenced the removal of some components of filling material (p<0.05), but not allowed for total cleaning. It was concluded that none of the irrigating solutions, associated or not to the use of ultrasound were able to completely clean the post space.
publisher Universidad de La Frontera. Facultad de Medicina
publishDate 2016
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2016000100007
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