The in vitro antifungal evaluation of a commercial extract of Chilean propolis against six fungi of agricultural importance
Propolis has been used for millennia for its antimicrobial and pharmaceutical properties, whereas its use as an agricultural antifungal agent has only recently been assessed. The chemical characteristics of six ethanolic extracts of Chilean propolis (EEP-1 to EEP-6) were evaluated. The total polyphenols ranged between 7.8 and 42.3 mg mL-1 equivalents of a 2:1 pinocembrin: galangin standard, and the total flavonoid contents ranged between 4.0 and 19.7 mg mL-1 equivalents of quercetin. A high-performance liquid chromatographic (HPLC) analysis allowed the identification of caffeic acid, myricetin, quercetin, kaempferol, apigenin, pinocembrin, galangin, caffeic acid phenethyl ester (CAPE) and rutin. The EEP-3 extract was selected for additional studies based on the higher total polyphenols and flavonoids contents (42.3 and 19.7 mg mL-1, respectively). The antifungal effects of four different concentrations (0.5, 1.0, 2.5 and 5.0 %) of EEP-3 on Alternaria alternata, Fusarium sp., Ulocladium sp., Botrytis cinerea, Penicillium expansum and Trichoderma reesei were evaluated in vitro using the agar dilution method. Although significant differences occurred among the extract concentrations, the results indicated that EEP-3 inhibited the mycelial growth of the six fungi evaluated.
Main Authors: | , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
2012
|
Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000200011 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S0718-16202012000200011 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S0718-162020120002000112012-10-24The in vitro antifungal evaluation of a commercial extract of Chilean propolis against six fungi of agricultural importanceCurifuta,MacarenaVidal,JorgeSánchez-Venegas,JaimeContreras,AliroSalazar,Luis AAlvear,Marysol Alternaria alternata antifungal activity Botrytis cinerea flavonoids Fusarium sp. Penicillium expansum polyphenols propolis Propolis has been used for millennia for its antimicrobial and pharmaceutical properties, whereas its use as an agricultural antifungal agent has only recently been assessed. The chemical characteristics of six ethanolic extracts of Chilean propolis (EEP-1 to EEP-6) were evaluated. The total polyphenols ranged between 7.8 and 42.3 mg mL-1 equivalents of a 2:1 pinocembrin: galangin standard, and the total flavonoid contents ranged between 4.0 and 19.7 mg mL-1 equivalents of quercetin. A high-performance liquid chromatographic (HPLC) analysis allowed the identification of caffeic acid, myricetin, quercetin, kaempferol, apigenin, pinocembrin, galangin, caffeic acid phenethyl ester (CAPE) and rutin. The EEP-3 extract was selected for additional studies based on the higher total polyphenols and flavonoids contents (42.3 and 19.7 mg mL-1, respectively). The antifungal effects of four different concentrations (0.5, 1.0, 2.5 and 5.0 %) of EEP-3 on Alternaria alternata, Fusarium sp., Ulocladium sp., Botrytis cinerea, Penicillium expansum and Trichoderma reesei were evaluated in vitro using the agar dilution method. Although significant differences occurred among the extract concentrations, the results indicated that EEP-3 inhibited the mycelial growth of the six fungi evaluated.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.39 n.2 20122012-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000200011en10.4067/S0718-16202012000200011 |
institution |
SCIELO |
collection |
OJS |
country |
Chile |
countrycode |
CL |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-cl |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Curifuta,Macarena Vidal,Jorge Sánchez-Venegas,Jaime Contreras,Aliro Salazar,Luis A Alvear,Marysol |
spellingShingle |
Curifuta,Macarena Vidal,Jorge Sánchez-Venegas,Jaime Contreras,Aliro Salazar,Luis A Alvear,Marysol The in vitro antifungal evaluation of a commercial extract of Chilean propolis against six fungi of agricultural importance |
author_facet |
Curifuta,Macarena Vidal,Jorge Sánchez-Venegas,Jaime Contreras,Aliro Salazar,Luis A Alvear,Marysol |
author_sort |
Curifuta,Macarena |
title |
The in vitro antifungal evaluation of a commercial extract of Chilean propolis against six fungi of agricultural importance |
title_short |
The in vitro antifungal evaluation of a commercial extract of Chilean propolis against six fungi of agricultural importance |
title_full |
The in vitro antifungal evaluation of a commercial extract of Chilean propolis against six fungi of agricultural importance |
title_fullStr |
The in vitro antifungal evaluation of a commercial extract of Chilean propolis against six fungi of agricultural importance |
title_full_unstemmed |
The in vitro antifungal evaluation of a commercial extract of Chilean propolis against six fungi of agricultural importance |
title_sort |
in vitro antifungal evaluation of a commercial extract of chilean propolis against six fungi of agricultural importance |
description |
Propolis has been used for millennia for its antimicrobial and pharmaceutical properties, whereas its use as an agricultural antifungal agent has only recently been assessed. The chemical characteristics of six ethanolic extracts of Chilean propolis (EEP-1 to EEP-6) were evaluated. The total polyphenols ranged between 7.8 and 42.3 mg mL-1 equivalents of a 2:1 pinocembrin: galangin standard, and the total flavonoid contents ranged between 4.0 and 19.7 mg mL-1 equivalents of quercetin. A high-performance liquid chromatographic (HPLC) analysis allowed the identification of caffeic acid, myricetin, quercetin, kaempferol, apigenin, pinocembrin, galangin, caffeic acid phenethyl ester (CAPE) and rutin. The EEP-3 extract was selected for additional studies based on the higher total polyphenols and flavonoids contents (42.3 and 19.7 mg mL-1, respectively). The antifungal effects of four different concentrations (0.5, 1.0, 2.5 and 5.0 %) of EEP-3 on Alternaria alternata, Fusarium sp., Ulocladium sp., Botrytis cinerea, Penicillium expansum and Trichoderma reesei were evaluated in vitro using the agar dilution method. Although significant differences occurred among the extract concentrations, the results indicated that EEP-3 inhibited the mycelial growth of the six fungi evaluated. |
publisher |
Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal |
publishDate |
2012 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000200011 |
work_keys_str_mv |
AT curifutamacarena theinvitroantifungalevaluationofacommercialextractofchileanpropolisagainstsixfungiofagriculturalimportance AT vidaljorge theinvitroantifungalevaluationofacommercialextractofchileanpropolisagainstsixfungiofagriculturalimportance AT sanchezvenegasjaime theinvitroantifungalevaluationofacommercialextractofchileanpropolisagainstsixfungiofagriculturalimportance AT contrerasaliro theinvitroantifungalevaluationofacommercialextractofchileanpropolisagainstsixfungiofagriculturalimportance AT salazarluisa theinvitroantifungalevaluationofacommercialextractofchileanpropolisagainstsixfungiofagriculturalimportance AT alvearmarysol theinvitroantifungalevaluationofacommercialextractofchileanpropolisagainstsixfungiofagriculturalimportance AT curifutamacarena invitroantifungalevaluationofacommercialextractofchileanpropolisagainstsixfungiofagriculturalimportance AT vidaljorge invitroantifungalevaluationofacommercialextractofchileanpropolisagainstsixfungiofagriculturalimportance AT sanchezvenegasjaime invitroantifungalevaluationofacommercialextractofchileanpropolisagainstsixfungiofagriculturalimportance AT contrerasaliro invitroantifungalevaluationofacommercialextractofchileanpropolisagainstsixfungiofagriculturalimportance AT salazarluisa invitroantifungalevaluationofacommercialextractofchileanpropolisagainstsixfungiofagriculturalimportance AT alvearmarysol invitroantifungalevaluationofacommercialextractofchileanpropolisagainstsixfungiofagriculturalimportance |
_version_ |
1755994618729594880 |