Genetic and physical map of broad host range cosmid pRG930cm
We hereby present the complete sequence and annotation of pRG930cm, a spectinomycin/streptomycin/chloramphenicol-resistant cosmid vector. pRG930cm (17,256 bp; GenBank Accession No.: FM174471) has a broad host range, and is stably maintained by a number of Gram-negative bacteria including Pseudomonas spp, Escherichia coli, Agrobacterium tumefaciens and Azorhizobium caulinodans ORS571. pRG930cm is already widely used and its sequence will aid efficient construction and analysis of cosmid libraries.
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Main Authors: | , , , , , |
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Format: | Digital revista |
Language: | English |
Published: |
Pontificia Universidad Católica de Valparaíso
2009
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Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200010 |
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