The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts
Abstract Background: Circular RNAs (circRNAs) has emerged as vital regulator involved in various diseases. In this study, we identified and investigated the potential circRNAs involved in gestational diabetes mellitus (GDM). Methods: High-throughput sequencing was used to collect the plasma circRNAs expression profiles of GDM patients. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was used to measure the expressions of circ_0008285 and circ_0001173 in the plasma specimens. The Pearson's correlation test was employed to assess the correlation between 2 circRNAs expression and the clinicopathologic data. Two circRNAs expression was verified in high glucose (HG)-induced HTR-8/SVneo cells. MTS, transwell assay was used to evaluate the effects of circ_0008285 expression on HG-induced HTR-8/SVneo cells. The network of circ_0008285 was constructed using cytocape. Results: In GDM patients, the expression of circ_0008285 was significantly upregulated, while that of circ_0001173 was decreased. Circ_0008285 was significantly correlated with the total cholesterol and LDL-C levels. Circ_0001173 was significantly correlated with glycated hemoglobin. HG promoted the proliferation, invasion, and migration in HTR-8/SVneo cells, while the knockdown of circ_0008285 exerted reverse effects. In addition, network construction exhibited that circ_0008285 had 45 miRNA binding sites, which correlated with 444 mRNA. Conclusions: circ_0008285 plays an important role and provides a clue for the usage of therapeutic targets in the development of GDM.
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Sociedad de Biología de Chile
2021
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oai:scielo:S0716-976020210001002142022-03-28The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblastsChen,HaitianZhang,ShaofengWu,YanxinLi,ZhuyuWang,DongyuCai,ShiqinWang,Zilian Circular RNA Gestational diabetes mellitus circ_0008285 Functional analysis Abstract Background: Circular RNAs (circRNAs) has emerged as vital regulator involved in various diseases. In this study, we identified and investigated the potential circRNAs involved in gestational diabetes mellitus (GDM). Methods: High-throughput sequencing was used to collect the plasma circRNAs expression profiles of GDM patients. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was used to measure the expressions of circ_0008285 and circ_0001173 in the plasma specimens. The Pearson's correlation test was employed to assess the correlation between 2 circRNAs expression and the clinicopathologic data. Two circRNAs expression was verified in high glucose (HG)-induced HTR-8/SVneo cells. MTS, transwell assay was used to evaluate the effects of circ_0008285 expression on HG-induced HTR-8/SVneo cells. The network of circ_0008285 was constructed using cytocape. Results: In GDM patients, the expression of circ_0008285 was significantly upregulated, while that of circ_0001173 was decreased. Circ_0008285 was significantly correlated with the total cholesterol and LDL-C levels. Circ_0001173 was significantly correlated with glycated hemoglobin. HG promoted the proliferation, invasion, and migration in HTR-8/SVneo cells, while the knockdown of circ_0008285 exerted reverse effects. In addition, network construction exhibited that circ_0008285 had 45 miRNA binding sites, which correlated with 444 mRNA. Conclusions: circ_0008285 plays an important role and provides a clue for the usage of therapeutic targets in the development of GDM.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.54 20212021-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100214en10.1186/s40659-021-00337-3 |
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Chen,Haitian Zhang,Shaofeng Wu,Yanxin Li,Zhuyu Wang,Dongyu Cai,Shiqin Wang,Zilian |
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Chen,Haitian Zhang,Shaofeng Wu,Yanxin Li,Zhuyu Wang,Dongyu Cai,Shiqin Wang,Zilian The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts |
| author_facet |
Chen,Haitian Zhang,Shaofeng Wu,Yanxin Li,Zhuyu Wang,Dongyu Cai,Shiqin Wang,Zilian |
| author_sort |
Chen,Haitian |
| title |
The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts |
| title_short |
The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts |
| title_full |
The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts |
| title_fullStr |
The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts |
| title_full_unstemmed |
The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts |
| title_sort |
role of circular rna circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts |
| description |
Abstract Background: Circular RNAs (circRNAs) has emerged as vital regulator involved in various diseases. In this study, we identified and investigated the potential circRNAs involved in gestational diabetes mellitus (GDM). Methods: High-throughput sequencing was used to collect the plasma circRNAs expression profiles of GDM patients. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was used to measure the expressions of circ_0008285 and circ_0001173 in the plasma specimens. The Pearson's correlation test was employed to assess the correlation between 2 circRNAs expression and the clinicopathologic data. Two circRNAs expression was verified in high glucose (HG)-induced HTR-8/SVneo cells. MTS, transwell assay was used to evaluate the effects of circ_0008285 expression on HG-induced HTR-8/SVneo cells. The network of circ_0008285 was constructed using cytocape. Results: In GDM patients, the expression of circ_0008285 was significantly upregulated, while that of circ_0001173 was decreased. Circ_0008285 was significantly correlated with the total cholesterol and LDL-C levels. Circ_0001173 was significantly correlated with glycated hemoglobin. HG promoted the proliferation, invasion, and migration in HTR-8/SVneo cells, while the knockdown of circ_0008285 exerted reverse effects. In addition, network construction exhibited that circ_0008285 had 45 miRNA binding sites, which correlated with 444 mRNA. Conclusions: circ_0008285 plays an important role and provides a clue for the usage of therapeutic targets in the development of GDM. |
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Sociedad de Biología de Chile |
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2021 |
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http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100214 |
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