Prenatal diagnosis of congenital rubella infection in São Paulo
Objective: rubella during the early stages of pregnancy can lead to severe birth defects known as congenital rubella syndrome (CRS). Samples collected from pregnant women with symptoms and suspected of congenital rubella infection between 1996 and 2008 were analyzed. Methods: a total of 23 amniotic fluid samples, 16 fetal blood samples, 1 product of conception and 1 placenta were analyzed by serology and RT-PCR. Results: all patients presented positive serology for IgG / IgM antibodies to rubella virus. Among neonates, 16 were IgG-positive, 9 were IgM-positive and 4 were negative for both antibodies. Of the 25 samples analyzed in this study, 24 were positive by RT-PCR. Changes in ultrasound were found in 15 (60%) of 25 fetuses infected with rubella virus. Fetal death and miscarriage were reported in 10 (40%) of the 25 cases analyzed. The rubella virus was amplified by PCR in all fetuses with abnormal ultrasound compatible with rubella. Fetal death and abortion were reported in 10 of 25 cases analyzed. Conclusion: this study, based on primary maternal rubella infection definitely confirms the good sensitivity and specificity of RT-PCR using amniotic fluid and ultrasound. The results showed that molecular assays are important tools in the early diagnosis of rubella and congenital rubella syndrome.
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Associação Médica Brasileira
2014
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oai:scielo:S0104-423020140005004512014-11-17Prenatal diagnosis of congenital rubella infection in São PauloCurti,Suely PiresFigueiredo,Cristina AdelaideOliveira,Maria Isabel deAndrade,Joelma QueirozZugaib,MarceloPedreira,Denise Araújo LapaDurigon,Edison Luiz ultrasonography prenatal rubella syndrome congenital amniotic fluid diagnosis RT-PCR serology Objective: rubella during the early stages of pregnancy can lead to severe birth defects known as congenital rubella syndrome (CRS). Samples collected from pregnant women with symptoms and suspected of congenital rubella infection between 1996 and 2008 were analyzed. Methods: a total of 23 amniotic fluid samples, 16 fetal blood samples, 1 product of conception and 1 placenta were analyzed by serology and RT-PCR. Results: all patients presented positive serology for IgG / IgM antibodies to rubella virus. Among neonates, 16 were IgG-positive, 9 were IgM-positive and 4 were negative for both antibodies. Of the 25 samples analyzed in this study, 24 were positive by RT-PCR. Changes in ultrasound were found in 15 (60%) of 25 fetuses infected with rubella virus. Fetal death and miscarriage were reported in 10 (40%) of the 25 cases analyzed. The rubella virus was amplified by PCR in all fetuses with abnormal ultrasound compatible with rubella. Fetal death and abortion were reported in 10 of 25 cases analyzed. Conclusion: this study, based on primary maternal rubella infection definitely confirms the good sensitivity and specificity of RT-PCR using amniotic fluid and ultrasound. The results showed that molecular assays are important tools in the early diagnosis of rubella and congenital rubella syndrome. info:eu-repo/semantics/openAccessAssociação Médica BrasileiraRevista da Associação Médica Brasileira v.60 n.5 20142014-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302014000500451en10.1590/1806-9282.60.05.013 |
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Curti,Suely Pires Figueiredo,Cristina Adelaide Oliveira,Maria Isabel de Andrade,Joelma Queiroz Zugaib,Marcelo Pedreira,Denise Araújo Lapa Durigon,Edison Luiz |
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Curti,Suely Pires Figueiredo,Cristina Adelaide Oliveira,Maria Isabel de Andrade,Joelma Queiroz Zugaib,Marcelo Pedreira,Denise Araújo Lapa Durigon,Edison Luiz Prenatal diagnosis of congenital rubella infection in São Paulo |
author_facet |
Curti,Suely Pires Figueiredo,Cristina Adelaide Oliveira,Maria Isabel de Andrade,Joelma Queiroz Zugaib,Marcelo Pedreira,Denise Araújo Lapa Durigon,Edison Luiz |
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Curti,Suely Pires |
title |
Prenatal diagnosis of congenital rubella infection in São Paulo |
title_short |
Prenatal diagnosis of congenital rubella infection in São Paulo |
title_full |
Prenatal diagnosis of congenital rubella infection in São Paulo |
title_fullStr |
Prenatal diagnosis of congenital rubella infection in São Paulo |
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Prenatal diagnosis of congenital rubella infection in São Paulo |
title_sort |
prenatal diagnosis of congenital rubella infection in são paulo |
description |
Objective: rubella during the early stages of pregnancy can lead to severe birth defects known as congenital rubella syndrome (CRS). Samples collected from pregnant women with symptoms and suspected of congenital rubella infection between 1996 and 2008 were analyzed. Methods: a total of 23 amniotic fluid samples, 16 fetal blood samples, 1 product of conception and 1 placenta were analyzed by serology and RT-PCR. Results: all patients presented positive serology for IgG / IgM antibodies to rubella virus. Among neonates, 16 were IgG-positive, 9 were IgM-positive and 4 were negative for both antibodies. Of the 25 samples analyzed in this study, 24 were positive by RT-PCR. Changes in ultrasound were found in 15 (60%) of 25 fetuses infected with rubella virus. Fetal death and miscarriage were reported in 10 (40%) of the 25 cases analyzed. The rubella virus was amplified by PCR in all fetuses with abnormal ultrasound compatible with rubella. Fetal death and abortion were reported in 10 of 25 cases analyzed. Conclusion: this study, based on primary maternal rubella infection definitely confirms the good sensitivity and specificity of RT-PCR using amniotic fluid and ultrasound. The results showed that molecular assays are important tools in the early diagnosis of rubella and congenital rubella syndrome. |
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Associação Médica Brasileira |
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2014 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302014000500451 |
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