Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean

Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean

In commercial plantations of soybean in both the Southern and the Cerrado regions, contributions from biological nitrogen fixation (BNF) are generally proportionately high. When using the 15N natural abundance technique to quantify BNF inputs, it is essential to determine, with accuracy, the 15N abundance of the N derived from BNF (the 'B' value). This study aimed to determine the effect of four recommended strains of Bradyrhizobium spp. (two B. japonicum and two B. elkanii) on the 'B' value of soybean grown in pots in an open field using an equation based on the determination of δ15N natural abundance in a non-labelled soil, and estimate of the contribution of BNF derived from the use of 15N-isotope dilution in soils enriched with 15N. To evaluate N2 fixation by soybean, three non-N2-fixing reference crops were grown under the same conditions. Regardless of Bradyrhizobium strain, no differences were observed in dry matter, nodule weight and total N between labelled and non-labelled soil. The N2 fixation of the soybeans grown in the two soil conditions were similar. The mean 'B' values of the soybeans inoculated with the B. japonicum strains were -1.84 ‰ and -0.50 ‰, while those inoculated with B. elkanii were -3.67 ‰ and -1.0 ‰, for the shoot tissue and the whole plant, respectively. Finally, the 'B' value for the soybean crop varied considerably in function of the inoculated Bradyrhizobium strain, being most important when only the shoot tissue was utilised to estimate the proportion of N in the plant derived from N2 fixation.

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Main Authors: Guimarães,Ana Paula, Morais,Rafael Fiusa de, Urquiaga,Segundo, Boddey,Robert Michael, Alves,Bruno José Rodrigues
Format: Digital revista
Language:English
Published: Escola Superior de Agricultura "Luiz de Queiroz" 2008
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500011
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spelling oai:scielo:S0103-901620080005000112008-09-16Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybeanGuimarães,Ana PaulaMorais,Rafael Fiusa deUrquiaga,SegundoBoddey,Robert MichaelAlves,Bruno José Rodrigues Bradyrhizobium elkanii Bradyrhizobium japonicum Glycine max 'B' value In commercial plantations of soybean in both the Southern and the Cerrado regions, contributions from biological nitrogen fixation (BNF) are generally proportionately high. When using the 15N natural abundance technique to quantify BNF inputs, it is essential to determine, with accuracy, the 15N abundance of the N derived from BNF (the 'B' value). This study aimed to determine the effect of four recommended strains of Bradyrhizobium spp. (two B. japonicum and two B. elkanii) on the 'B' value of soybean grown in pots in an open field using an equation based on the determination of δ15N natural abundance in a non-labelled soil, and estimate of the contribution of BNF derived from the use of 15N-isotope dilution in soils enriched with 15N. To evaluate N2 fixation by soybean, three non-N2-fixing reference crops were grown under the same conditions. Regardless of Bradyrhizobium strain, no differences were observed in dry matter, nodule weight and total N between labelled and non-labelled soil. The N2 fixation of the soybeans grown in the two soil conditions were similar. The mean 'B' values of the soybeans inoculated with the B. japonicum strains were -1.84 ‰ and -0.50 ‰, while those inoculated with B. elkanii were -3.67 ‰ and -1.0 ‰, for the shoot tissue and the whole plant, respectively. Finally, the 'B' value for the soybean crop varied considerably in function of the inoculated Bradyrhizobium strain, being most important when only the shoot tissue was utilised to estimate the proportion of N in the plant derived from N2 fixation.info:eu-repo/semantics/openAccessEscola Superior de Agricultura "Luiz de Queiroz"Scientia Agricola v.65 n.5 20082008-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500011en10.1590/S0103-90162008000500011
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Guimarães,Ana Paula
Morais,Rafael Fiusa de
Urquiaga,Segundo
Boddey,Robert Michael
Alves,Bruno José Rodrigues
spellingShingle Guimarães,Ana Paula
Morais,Rafael Fiusa de
Urquiaga,Segundo
Boddey,Robert Michael
Alves,Bruno José Rodrigues
Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean
author_facet Guimarães,Ana Paula
Morais,Rafael Fiusa de
Urquiaga,Segundo
Boddey,Robert Michael
Alves,Bruno José Rodrigues
author_sort Guimarães,Ana Paula
title Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean
title_short Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean
title_full Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean
title_fullStr Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean
title_full_unstemmed Bradyrhizobium strain and the 15N natural abundance quantification of biological N2 fixation in soybean
title_sort bradyrhizobium strain and the 15n natural abundance quantification of biological n2 fixation in soybean
description In commercial plantations of soybean in both the Southern and the Cerrado regions, contributions from biological nitrogen fixation (BNF) are generally proportionately high. When using the 15N natural abundance technique to quantify BNF inputs, it is essential to determine, with accuracy, the 15N abundance of the N derived from BNF (the 'B' value). This study aimed to determine the effect of four recommended strains of Bradyrhizobium spp. (two B. japonicum and two B. elkanii) on the 'B' value of soybean grown in pots in an open field using an equation based on the determination of δ15N natural abundance in a non-labelled soil, and estimate of the contribution of BNF derived from the use of 15N-isotope dilution in soils enriched with 15N. To evaluate N2 fixation by soybean, three non-N2-fixing reference crops were grown under the same conditions. Regardless of Bradyrhizobium strain, no differences were observed in dry matter, nodule weight and total N between labelled and non-labelled soil. The N2 fixation of the soybeans grown in the two soil conditions were similar. The mean 'B' values of the soybeans inoculated with the B. japonicum strains were -1.84 ‰ and -0.50 ‰, while those inoculated with B. elkanii were -3.67 ‰ and -1.0 ‰, for the shoot tissue and the whole plant, respectively. Finally, the 'B' value for the soybean crop varied considerably in function of the inoculated Bradyrhizobium strain, being most important when only the shoot tissue was utilised to estimate the proportion of N in the plant derived from N2 fixation.
publisher Escola Superior de Agricultura "Luiz de Queiroz"
publishDate 2008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500011
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