Vitamin C and total phenols quantification in calli of native passion fruit induced by combinations of Picloram and Kinetin

Vitamin C and total phenols quantification in calli of native passion fruit induced by combinations of Picloram and Kinetin

Brazil is one of the center of origin of passion fruit and has an important natural variability of the genus Passiflora. Several wild species of this genus are resistant to some pests and diseases and many are considered as medicinal. The aim of this research was to induce callus from in vitro Passiflora gibertiileaf explants for quantification of vitamin C and total phenols. Once the appropriate auxin/cytokine balance promotes callus formation and may optimize the production of secondary compounds and vitamins, calli were induced using a half-strength MS medium with a combination of the auxin Picloran (PIC) and the cytokine Kinetin (KIN). The vitamin C and total phenols were quantified by colorimetric methods from calli after different culture periods. The calli induction was strongly dependent of the combination PIC/KIN. It was observed high vitamin C content (94.8mg 100g-1) during the calli induction period in MS+4.14µM PIC+ 0.207µM KIN. Higher PIC/KIN concentrations promoted an increase in the vitamin C content after three subcultures. The higher PIC (8.28µM)/KIN (0.828µM) concentration was the higher was the total phenols production (66mg tannic acid 100g-1of fresh callus) during the calli induction period.

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Main Authors: Artioli-Coelho,Fabiane Aparecida, Paiva,Renato, Silva,Luciano Coutinho, Barbosa,Sandro, Beijo,Luiz Alberto
Format: Digital revista
Language:English
Published: Universidade Federal de Santa Maria 2015
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801459
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spelling oai:scielo:S0103-847820150008014592015-08-31Vitamin C and total phenols quantification in calli of native passion fruit induced by combinations of Picloram and KinetinArtioli-Coelho,Fabiane AparecidaPaiva,RenatoSilva,Luciano CoutinhoBarbosa,SandroBeijo,Luiz Alberto Passiflora gibertii native species medicinal plant secondary compounds. Brazil is one of the center of origin of passion fruit and has an important natural variability of the genus Passiflora. Several wild species of this genus are resistant to some pests and diseases and many are considered as medicinal. The aim of this research was to induce callus from in vitro Passiflora gibertiileaf explants for quantification of vitamin C and total phenols. Once the appropriate auxin/cytokine balance promotes callus formation and may optimize the production of secondary compounds and vitamins, calli were induced using a half-strength MS medium with a combination of the auxin Picloran (PIC) and the cytokine Kinetin (KIN). The vitamin C and total phenols were quantified by colorimetric methods from calli after different culture periods. The calli induction was strongly dependent of the combination PIC/KIN. It was observed high vitamin C content (94.8mg 100g-1) during the calli induction period in MS+4.14µM PIC+ 0.207µM KIN. Higher PIC/KIN concentrations promoted an increase in the vitamin C content after three subcultures. The higher PIC (8.28µM)/KIN (0.828µM) concentration was the higher was the total phenols production (66mg tannic acid 100g-1of fresh callus) during the calli induction period.info:eu-repo/semantics/openAccessUniversidade Federal de Santa MariaCiência Rural v.45 n.8 20152015-08-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801459en10.1590/0103-8478cr20120965
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Artioli-Coelho,Fabiane Aparecida
Paiva,Renato
Silva,Luciano Coutinho
Barbosa,Sandro
Beijo,Luiz Alberto
spellingShingle Artioli-Coelho,Fabiane Aparecida
Paiva,Renato
Silva,Luciano Coutinho
Barbosa,Sandro
Beijo,Luiz Alberto
Vitamin C and total phenols quantification in calli of native passion fruit induced by combinations of Picloram and Kinetin
author_facet Artioli-Coelho,Fabiane Aparecida
Paiva,Renato
Silva,Luciano Coutinho
Barbosa,Sandro
Beijo,Luiz Alberto
author_sort Artioli-Coelho,Fabiane Aparecida
title Vitamin C and total phenols quantification in calli of native passion fruit induced by combinations of Picloram and Kinetin
title_short Vitamin C and total phenols quantification in calli of native passion fruit induced by combinations of Picloram and Kinetin
title_full Vitamin C and total phenols quantification in calli of native passion fruit induced by combinations of Picloram and Kinetin
title_fullStr Vitamin C and total phenols quantification in calli of native passion fruit induced by combinations of Picloram and Kinetin
title_full_unstemmed Vitamin C and total phenols quantification in calli of native passion fruit induced by combinations of Picloram and Kinetin
title_sort vitamin c and total phenols quantification in calli of native passion fruit induced by combinations of picloram and kinetin
description Brazil is one of the center of origin of passion fruit and has an important natural variability of the genus Passiflora. Several wild species of this genus are resistant to some pests and diseases and many are considered as medicinal. The aim of this research was to induce callus from in vitro Passiflora gibertiileaf explants for quantification of vitamin C and total phenols. Once the appropriate auxin/cytokine balance promotes callus formation and may optimize the production of secondary compounds and vitamins, calli were induced using a half-strength MS medium with a combination of the auxin Picloran (PIC) and the cytokine Kinetin (KIN). The vitamin C and total phenols were quantified by colorimetric methods from calli after different culture periods. The calli induction was strongly dependent of the combination PIC/KIN. It was observed high vitamin C content (94.8mg 100g-1) during the calli induction period in MS+4.14µM PIC+ 0.207µM KIN. Higher PIC/KIN concentrations promoted an increase in the vitamin C content after three subcultures. The higher PIC (8.28µM)/KIN (0.828µM) concentration was the higher was the total phenols production (66mg tannic acid 100g-1of fresh callus) during the calli induction period.
publisher Universidade Federal de Santa Maria
publishDate 2015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801459
work_keys_str_mv AT artiolicoelhofabianeaparecida vitamincandtotalphenolsquantificationincalliofnativepassionfruitinducedbycombinationsofpicloramandkinetin
AT paivarenato vitamincandtotalphenolsquantificationincalliofnativepassionfruitinducedbycombinationsofpicloramandkinetin
AT silvalucianocoutinho vitamincandtotalphenolsquantificationincalliofnativepassionfruitinducedbycombinationsofpicloramandkinetin
AT barbosasandro vitamincandtotalphenolsquantificationincalliofnativepassionfruitinducedbycombinationsofpicloramandkinetin
AT beijoluizalberto vitamincandtotalphenolsquantificationincalliofnativepassionfruitinducedbycombinationsofpicloramandkinetin
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