Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp Cells
Abstract Prostaglandin E2 (PGE2) is a lipid mediator usually released during inflammation. This study aimed to investigate the potential of soluble or microsphere-loaded PGE2 on inducing differentiation of dental pulp stem cells. PGE2-loaded microspheres (MS) were prepared using an oil-in-water emulsion solvent extraction-evaporation process and were characterized. Mouse dental pulp stem cells (OD-21) were stimulated with soluble or PGE2-loaded MS (0.01 and 0.1 µM). Cell viability was determined by MTT colorimetric assay. Ibsp, Bmp2 and Runx2 expression was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) after 3, 6, and 24 h. The results showed that the soluble PGE2 reduced dental pulp stem cells viability after 24 h of stimulation whereas PGE2-loaded MS did not. Soluble PGE2 up-regulated Ibsp and Bmp2 at 3 h, differently from PGE2-loaded MS. On the other hand, PGE2-MS induced Bmp2 and Runx2 at 6 h and Ibsp at 24 h. In conclusion, our in vitro results show that PGE2, soluble or loaded in MS are not cytotoxic and modulateIbsp,Bmp2, andRunx2gene expression in cultured OD-21 cells.
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Fundação Odontológica de Ribeirão Preto
2019
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oai:scielo:S0103-644020190003002012019-06-06Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp CellsLorencetti-Silva,FrancinePereira,Priscilla Aparecida TartariMeirelles,Alyne Fávero GalvãoFaccioli,Lúcia HelenaPaula-Silva,Francisco Wanderley Garcia microspheres prostaglandin E2 dental pulp stem cell differentiation Abstract Prostaglandin E2 (PGE2) is a lipid mediator usually released during inflammation. This study aimed to investigate the potential of soluble or microsphere-loaded PGE2 on inducing differentiation of dental pulp stem cells. PGE2-loaded microspheres (MS) were prepared using an oil-in-water emulsion solvent extraction-evaporation process and were characterized. Mouse dental pulp stem cells (OD-21) were stimulated with soluble or PGE2-loaded MS (0.01 and 0.1 µM). Cell viability was determined by MTT colorimetric assay. Ibsp, Bmp2 and Runx2 expression was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) after 3, 6, and 24 h. The results showed that the soluble PGE2 reduced dental pulp stem cells viability after 24 h of stimulation whereas PGE2-loaded MS did not. Soluble PGE2 up-regulated Ibsp and Bmp2 at 3 h, differently from PGE2-loaded MS. On the other hand, PGE2-MS induced Bmp2 and Runx2 at 6 h and Ibsp at 24 h. In conclusion, our in vitro results show that PGE2, soluble or loaded in MS are not cytotoxic and modulateIbsp,Bmp2, andRunx2gene expression in cultured OD-21 cells.info:eu-repo/semantics/openAccessFundação Odontológica de Ribeirão PretoBrazilian Dental Journal v.30 n.3 20192019-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000300201en10.1590/0103-6440201902542 |
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Lorencetti-Silva,Francine Pereira,Priscilla Aparecida Tartari Meirelles,Alyne Fávero Galvão Faccioli,Lúcia Helena Paula-Silva,Francisco Wanderley Garcia |
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Lorencetti-Silva,Francine Pereira,Priscilla Aparecida Tartari Meirelles,Alyne Fávero Galvão Faccioli,Lúcia Helena Paula-Silva,Francisco Wanderley Garcia Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp Cells |
author_facet |
Lorencetti-Silva,Francine Pereira,Priscilla Aparecida Tartari Meirelles,Alyne Fávero Galvão Faccioli,Lúcia Helena Paula-Silva,Francisco Wanderley Garcia |
author_sort |
Lorencetti-Silva,Francine |
title |
Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp Cells |
title_short |
Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp Cells |
title_full |
Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp Cells |
title_fullStr |
Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp Cells |
title_full_unstemmed |
Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp Cells |
title_sort |
prostaglandin e2 induces expression of mineralization genes by undifferentiated dental pulp cells |
description |
Abstract Prostaglandin E2 (PGE2) is a lipid mediator usually released during inflammation. This study aimed to investigate the potential of soluble or microsphere-loaded PGE2 on inducing differentiation of dental pulp stem cells. PGE2-loaded microspheres (MS) were prepared using an oil-in-water emulsion solvent extraction-evaporation process and were characterized. Mouse dental pulp stem cells (OD-21) were stimulated with soluble or PGE2-loaded MS (0.01 and 0.1 µM). Cell viability was determined by MTT colorimetric assay. Ibsp, Bmp2 and Runx2 expression was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) after 3, 6, and 24 h. The results showed that the soluble PGE2 reduced dental pulp stem cells viability after 24 h of stimulation whereas PGE2-loaded MS did not. Soluble PGE2 up-regulated Ibsp and Bmp2 at 3 h, differently from PGE2-loaded MS. On the other hand, PGE2-MS induced Bmp2 and Runx2 at 6 h and Ibsp at 24 h. In conclusion, our in vitro results show that PGE2, soluble or loaded in MS are not cytotoxic and modulateIbsp,Bmp2, andRunx2gene expression in cultured OD-21 cells. |
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Fundação Odontológica de Ribeirão Preto |
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2019 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000300201 |
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1756404618278993920 |